Maximizing PCR and RT-PCR Success - Summary

PCR Brochure 10/2016 21 In addition, the new QIAGEN OneStep Ahead RT-PCR Kit adds increased process safety and convenience to viral detection. The RT-blocker keeps the reverse transcription enzyme inactivated at ambient temperatures and enables room-temperature reaction setup, as well as high-throughput use in automated workflows. Its inbuilt RNase inhibitor protects the sample from RNase contamination and the optional use of dual-color pipetting control (Figure 25) reduces the risk of human pipetting error. The kit comes in a convenient master mix format with an ultrafast cycling protocol that takes just 1 hour. For improved sequence accuracy and amplification of long fragments up to 4 kb, the mix also includes a high fidelity/proofreading polymerase, which makes the kit particularly useful for protocols including subsequent sequencing of targets. Figure 25. Dual-color pipetting control in the QIAGEN OneStep Ahead RT-PCR Kit. See how the control works here. Summary PCR is a powerful and still-developing tool for modern molecular biology. Recent advances in PCR have enabled increased sensitivity and specificity coupled with even faster results. Following the simple guidelines presented in this brochure will allow you to achieve optimal PCR results – even at your first attempt. QIAGEN PCR and RT-PCR products have been specifically developed to provide superior results without the need for optimization. In addition, our range of products offer convenient features, such as master mix formats, gel-loading PCR buffers and streamlined protocols, as well as automation for every step of the workflow – from setup to detection. We also provide a range of reliable quantitative PCR and RT-PCR kits, as well as whole genome amplification kits. Visit www.qiagen.com/PCR-literature and find out more about overcoming challenges in end-point PCR with QIAGEN’s PCR solutions. Whatever your PCR application, QIAGEN has a kit to suit your needs (see selection guides, page 23–25).

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