Maximizing PCR and RT-PCR Success

PCR Brochure 10/2016 13 One protocol for all assays QIAGEN’s unique dual-cation buffer system – provided with every QIAGEN PCR and RT-PCR kit – ensures highly specific amplification, resulting in significant time and cost savings. No time-consuming optimization or gradient PCR is required for the development and verification of new assays; successful results can be easily obtained using just a single, optimized PCR protocol that can be programmed into the thermocycler (Figure 15). Significant time and cost savings The simple reaction setup, fast procedure, consistent results, and ease of use ensured by QIAGEN PCR kits result in substantial savings in time and costs, as well as reagents, because optimization of PCR parameters is no longer necessary. The need for repeated experiments is eliminated and PCR success is achieved at the first attempt. PCR assay development is straightforward and easy, using a single, optimized protocol (Figure 16). Figure 14. Integrated loading dye for streamlined handling. A CoralLoad® PCR Buffer, provided with many QIAGEN PCR kits, contains gel-tracking dyes for easier pipetting, B enabling immediate gel loading of PCR samples and easy visualization of DNA migration. Figure 15. High PCR product yields without the need for optimization. TopTaq DNA Polymerase was used to amplify 14 different PCR products from mammalian genomic DNA ranging in size from 100 bp to 2 kb according to the standard preoptimized protocol and using identical cycling conditions. Figure 16. Successful PCR without the need for optimization. QIAGEN PCR kits provide a single, straightforward procedure for rapid and reliable results. In contrast to current methods, QIAGEN PCR kits eliminate the need for optimization of PCR parameters, making assay development fast and easy. 10 1 2 3 4 5 6 7 8 9 11 12 13 14 11 12 13 14 11 12 13 14 1112 1314 11 12 13 14 QIAGEN M M QIAGEN Supplier A Supplier I Supplier T Successful PCR results without optimization Follow kit protocol Develop strategy Optimization of all PCR parameters PCR analysis only after lengthy optimization Time Time QIAGEN Multiplex PCR Plus Kit Current method 12345678911121314 11121314111213141112131411121314

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