Investigator ESSplex SE QS Kit

The Invesigator ESSplex SE QS Kit utilizes fast-cycling technology to provide reliable results in approximately 60 minutes.
The novel Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.
The Investigator ESSplex SE QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

The Investigator ESSplex SE QS Kit uses QIAGEN's fast-cycling PCR technology for the simultaneous amplification of 16 STR markers and Amelogenin, as recommended by the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling Group (EDNAP) as the European Standard Set of loci (ESS).

The Investigator ESSplex SE QS Kit Primer Mix contains the new innovative performance control Quality Sensor (QS1 and QS2). It provides additional information on sample quality and PCR success. The Quality Sensor, being simultaneously amplified with the STR markers, shows you if you are dealing with:

• Successful PCR amplification
• Failed PCR amplification
• Absence of DNA
• Degraded DNA
• Inhibitors

The additional information obtained with the Quality Sensor will help you streamline your sample analysis by showing you where to focus your analytical efforts, and save you time and money by eliminating the need for unnecessary PCR re-runs.

The 16 STR markers, Amelogenin and the Quality Sensor are amplified simultaneously in a single PCR, and labeled with one of the dyes listed in Table 1 "Investigator ESSplex SE QS Kit dyes and corresponding markers". 
The loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as the Applied Biosystems GeneMapper ID-X. See the table "Investigator ESSplex SE QS Kit technical specifications" for more information.
 

Table 1. Investigator ESSplex SE QS Kit dyes and corresponding markers

Dye	Marker
6-FAM	QS1	Amelogenin	TH01	D3S1358	vWA	D21S11	QS2
BTG	D163539	D1S1656	D19S433	SE33
BTY	D10S1248	D22S1045	D12S391	D8S1179	D2S1338
BTR	D2S441	D18S51	FGA

Table 2. Investigator ESSplex SE QS Kit technical specifications

Category	Details
Volume per PCR	25 µl
Matrix	BT5
Fluorescence labels	6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers	ABI PRISMR 3100/3100-Avant, Applied Biosystems 3130/3130xl or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis	Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software

The Investigator ESSplex SE QS Kit enables simultaneous amplification of the European Standard Set (ESS), including SE33, and is highly suitable for:

• Criminal database and forensic casework samples
• Paternity testing
• Population genetics studies
• Other human identity testing applications