TissueRuptor II

S_0161_TisRup024_light
For low-throughput sample disruption for molecular analysis
  • Rapid, effective disruption of a range of sample types
  • Disposable probes help to minimize cross-contamination
  • Time savings through use of disposable probes
  • Visual monitoring of disruption using transparent probes
  • Seamless integration with QIAGEN sample technologies
The TissueRuptor II is a handheld rotor-stator homogenizer that provides rapid, efficient and flexible disruption of human, animal and plant samples for a wide range of downstream applications. The TissueRuptor II uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.

The TissueRuptor II has all the same features and functionality as the old TissueRuptor.
製品名 Cat. no. List price:
TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
990890
¥39,000
TissueRuptor (120 V, 50/60 Hz, JP)
Handheld rotor–stator homogenizer, 120 V, 50/60 Hz (for Japan), 5 TissueRuptor Disposable Probes
9002710
¥102,000
TissueRuptor II (100 V, 50/60 Hz, JP)

Handheld rotor–stator homogenizer, 100 V, 50/60 Hz (for Japan), 5 TissueRuptor Disposable Probes

9002754
¥102,000

TissueRuptor II  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


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Reproducible purification of high-quality genomic DNA.|Efficient disruption and homogenization of animal tissues.|Successful purification of phosphoproteins.|High performance results in PCR.|Pure RNA with high RIN values.|
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.|The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.|Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.|[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.|Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.|
Performance
QIAGEN has tested use of the TissueRuptor II in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor II ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting and enzyme assays (see figures "Successful purification of phosphoprotein " and "High performance results in PCR"). For most tissues, disruption and homogenization using the TissueRuptor II gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor II allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values," "Reproducible purification of high-quality genomic DNA" and "Efficient disruption and homogenization of animal tissues").
Principle

Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. The TissueRuptor II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor II is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAprotect Tissue Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified Sample type QIAGEN kit
RNA Easy-to-lyse tissue

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits
RNA All types of tissue RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA Plant tissue RNeasy Plant Mini Kit
RNA Yeast RNeasy Kits
RNA Bacteria RNeasy Protect Bacteria Kits
microRNA All types of tissue miRNeasy Kits
DNA Human tissue QIAamp DNA Kits
DNA Animal tissue DNeasy Blood & Tissue Kits
Protein Tissue Qproteome Mammalian Protein Prep Kit
Phosphoprotein Tissue PhosphoProtein Purification Kit
Glycoprotein Tissue Qproteome Glycoprotein Kits
DNA and RNA Tissue AllPrep DNA/RNA Kits
DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Mini Kit

 

Procedure

The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

Applications

The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits.

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詳細を表示
ディスポーザブルプローブを用いる、低スループットの生体サンプルを破砕
詳細を表示
For low-throughput disruption of biological samples using disposable probes
詳細を表示
機器ユーザーマニュアル
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Reproducible purification of high-quality genomic DNA
再現性と品質の高いゲノムDNA精製
凍結肝臓および腎臓サンプルは (それぞれ30 mg) 溶解バッファー中で TissueRuptor を用いて最高速度で30秒間破砕した。AllPrep DNA/RNA Mini Kit を用いてゲノムDNAの精製を行ない、アガロースゲル電気泳動で分析した。M:マーカー。
Efficient disruption and homogenization of animal tissues
動物組織の効率的な破砕とホモジナイゼーション
TissueRuptor を用いて最高速度で30秒間次の組織を破砕した:凍結した腎臓、肝臓、脾臓 (各10 mg);凍結肺 (250 mg);安定化した心臓、筋肉、脳 (各10 mg)。サンプルは溶解バッファー (腎臓、肝臓、脾臓、肺、心臓、筋肉) あるいはQIAzol Lysis Reagent (脳) 内で破砕した。RNeasy Plus Mini Kit(RNeasy Plus)、RNeasy Midi Kit(RNeasy Midi)、RNeasy Fibrous Tissue Mini Kit(RNeasy Fibrous)、RNeasy Lipid Tissue Mini Kit(RNeasy Lipid)を用いてトータルRNAを精製した。精製したRNAをホルムアルデヒドアガロースゲル電気泳動で分析した。ゲル写真はTissueRuptor を用いて破砕後に精製したRNA収量および品質が高いことを示している。
G_0179_TR
リン酸化タンパク質の精製
凍結した肝臓および脳 (約30 mg) を350 μl のPhosphoProtein Lysis Buffer 中で30~60秒 (中間速度)間破砕した。ホモジナイズしたサンプルを1500 μl のPhosphoProtein Lysis Bufferで希釈し、10分毎にボルテックスで簡単に混合し4℃で30分間インキュベートした。リン酸化タンパク質はPhosphoProtein Purification Kit を用いて精製し、ウェスタンブロットで検出した。[A] Ponceau Sで染色したトランスファーしたメンブレン、[B] ウェスタンブロット。M:マーカー; FT:フロースルー、E3:溶出画分3。
High performance results in PCR
PCRで良好な結果
[A] 肝臓と[B]心臓の凍結サンプル (各25 mg) をTissueRuptor あるいは従来のローターステーターホモジナイザーを用いて破砕し、proteinase Kと共に1時間溶解するか、あるいはサンプルの破砕なしにproteinase Kと共に一晩溶解した。QIAamp DNA Mini Kit を用いてDNAを精製した。HotStarTaq DNA Polymerase と18SリボソームRNA遺伝子に特異的なプライマーを用いて溶出液(5 μl)からPCRを行なった。M:GelPilot Mid Range Ladder。
Pure RNA with high RIN values
高いRIN値の高純度RNA
RNA をディスポーザブルプローブをセットしたTissueRuptor あるいはスチール製ジェネレーターシャフトのローターステーター式ホモジナイザーを用いて、凍結肝臓サンプル(各30 mg)を最高速度で30 秒間破砕した。RNeasy PlusMini Kit を用いてトータルRNA を精製し、Agilent 2100 Bioanalyzerで解析した。高いRIN (RNA Integrity Number) 値はRNAが高品質であることを示している。