QuantiTect Primer Assays

For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
  • Guaranteed results with genomewide, predesigned primers
  • ~100% PCR efficiencies for reliable relative quantification
  • High sensitivity and specificity
  • Accurate quantification over a wide linear range
  • Time and cost savings with SYBR Green detection

QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.

产品 货号 目录价:
QuantiTect Primer Assay (200)
For 200 x 50 µl reactions or 400 x 25 µl reactions: 10x QuantiTect Primer Assay (lyophilized) supplied in single tube
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Details

QuantiTect Primer Assays适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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real-time RT-PCR具有高度灵敏性。|real-time RT-PCR具有可重复性。|研发real-time RT-PCR试剂时节省时间。|
使用 [A] QuantiTect Primer Assay和QuantiTect SYBR® Green PCR Kit或者 [B] Supplier AII的基于探针的real-time PCR试剂盒,定量检测人白细胞cDNA (1 ng) 中的MAPK1 (一种蛋白激酶) 和IL6 (一种细胞因子),重复两次。QuantiTect Primer Assay的灵敏度高于基于探针的分析,表现为其CT值较低,且具有高特异性,如图中熔解曲线分析中的单峰所示。|[A] 使用针对IL8 (一种趋化因子) 的QuantiTect Primer Assay和QuantiFast SYBR® Green PCR Kit分析不同量的白细胞cDNA,重复两次。同时进行无模板对照 (NTC) 反应 (平直曲线)。[B] 熔解曲线分析证明了高PCR特异性。|在实验室自行研发RT-PCR试剂时,QuantiTect Primer Assays能够显著节省时间。|
性能

QuantiTect Primer Assays enable reproducible quantification of RNA transcripts over a range of template amounts (see part [A] of figure "Reproducible real-time RT-PCR"). Accurate quantification is guaranteed when the assays are run using QuantiFast SYBR Green Kits or other SYBR Green kits from QIAGEN. The kits prevent the formation of PCR artifacts (see part [B] of figure "Reproducible real-time RT-PCR").

When used with QuantiFast, QuantiTect, Rotor-Gene, or FastLane SYBR Green Kits (master mixes for two-step or one-step RT-PCR), the assays may even give lower CT values compared with probe-based assays (see figure "Superior sensitivity in real-time RT-PCR"). This is due to the high PCR specificity of the kits and multiple binding of SYBR Green dye to each PCR product. Highly sensitive quantification is possible even with low-abundance transcripts.

原理
QuantiTect Primer Assays eliminate the need to develop and validate real-time RT-PCR assays (see figure "Time savings in development of real-time RT-PCR assays"). They can be used in both one-step and two-step qRT-PCR. Simply visit QIAGEN's GeneGlobe Web portal, and select and order an assay for any gene from human, rat, mouse, Drosophila, Arabidopsis, and other species. QuantiTect Primer Assays provide guaranteed results when used with QuantiFast, QuantiTect, Rotor-Gene, or FastLane SYBR Green Kits (master mixes for two-step or one-step RT-PCR).
操作流程

Each QuantiTect Primer Assay is supplied as a lyophilized mix of forward and reverse primers for a specific gene. The primers are reconstituted in TE, pH 8.0 to give a 10x primer solution, which is then added to the master mix supplied with a QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kit for SYBR Green detection. The master mix with primers is aliquoted into PCR tubes or wells, and the individual template samples are then added.

QuantiTect Primer Assays provide guaranteed results in gene expression analysis when used in combination with one of the following kits shown in the table.

Two-step and one-step qRT-PCR kits for use with QuantiTect Primer Assays
Two-step qRT-PCROne-step qRT-PCR
QuantiFast SYBR Green PCR Kit QuantiFast SYBR Green RT-PCR Kit
Rotor-Gene SYBR Green PCR Kit Rotor-Gene SYBR Green RT-PCR Kit
QuantiTect SYBR Green PCR Kit QuantiTect SYBR Green RT-PCR Kit

QuantiTect Primer Assays are designed to detect RNA only where possible. For assays where detection of genomic DNA cannot be avoided, cDNA can be prepared using the QuantiTect Reverse Transcription Kit or the FastLane Cell cDNA Kit. Both kits provide cDNA synthesis with integrated removal of genomic DNA contamination.

Naming

Each QuantiTect Primer Assay available for a particular gene is given a number that appears in the product name after the gene symbol. Read more in the GeneGlobe FAQs.

应用

QuantiTect Primer Assays are for gene expression analysis using one-step and two-step qRT-PCR with SYBR Green detection. They are highly suited for applications such as:

  • Validation of siRNA-mediated gene knockdown
  • Validation of microarray results
  • High-throughput Screening

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试剂盒操作手册
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For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection
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QuantiTect Primer Assays for housekeeping genes can be used as endogenous controls in qRT-PCR.
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安全数据表
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Download Safety Data Sheets for QIAGEN product components.
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安全数据表
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图片
Superior sensitivity in real-time RT-PCR on the StepOnePlus.
real-time RT-PCR具有高度灵敏性。
使用 [A] QuantiTect Primer Assay和QuantiTect SYBR® Green PCR Kit或者 [B] Supplier AII的基于探针的real-time PCR试剂盒,定量检测人白细胞cDNA (1 ng) 中的MAPK1 (一种蛋白激酶) 和IL6 (一种细胞因子),重复两次。QuantiTect Primer Assay的灵敏度高于基于探针的分析,表现为其CT值较低,且具有高特异性,如图中熔解曲线分析中的单峰所示。
Reproducible real-time RT-PCR on the iCycler iQ.
real-time RT-PCR具有可重复性。
[A] 使用针对IL8 (一种趋化因子) 的QuantiTect Primer Assay和QuantiFast SYBR® Green PCR Kit分析不同量的白细胞cDNA,重复两次。同时进行无模板对照 (NTC) 反应 (平直曲线)。[B] 熔解曲线分析证明了高PCR特异性。
Time savings in development of real-time RT-PCR assays.
研发real-time RT-PCR试剂时节省时间。
在实验室自行研发RT-PCR试剂时,QuantiTect Primer Assays能够显著节省时间。