For the isolation of DNA from up to 384 soil samples in less than one day
Fast, high-throughput format processes up to 250 mg of sample in 96 wells
Easy isolation of DNA from tough microbes using the PowerLyzer 24 Homogenizer
Completely eliminates PCR inhibitors with Inhibitor Removal Technology
Processing large amounts of soil and other environmental samples is fast and easy with the DNeasy PowerSoil HTP 96 Kit. Using a 96-well format, difficult soil types from compost to marine sediment to manure can be successfully processed and the DNA isolated. The highly pure DNA resulting from processing with the DNeasy PowerSoil HTP 96 Kit is ready for downstream applications, including qPCR amplification.
Samples are broken down and homogenized on the 96-well bead beating plate. Cells are lysed by both mechanical and chemical means and the DNA is captured on a 96-well silica membrane spin column, washed and eluted. The DNA is then ready for downstream applications such as qPCR or next-generation sequencing.
PCR analysis on resulting DNA has detected a variety of organisms, from yeasts, molds and other fungi, algae, Gram (+/-) bacteria (including Bacillus subtilis, Bacillus anthracis), actinomycetes (including Streptomyces) and nematodes.
DNeasy PowerSoil HTP 96 Kit was formerly sold by MO BIO as PowerSoil-htp 96 Well Soil DNA Kit.
The DNeasy PowerSoil HTP 96 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
Specifications
Bead size
0.7 mm garnet
Binding capacity
Up to 20 µg per prep
Format
Silica Spin Filter Plates
Processing
Bead beating
Sample size
0.25 g
Sample types
Processed soil, fecal, water, food, insects, swabs with PCR inhibitors
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)
