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BioSprint 96 DNA Plant Kit

For rapid and economical automated purification of total DNA from plant tissue
  • Complete removal of contamination and inhibitors
  • Pure DNA, ready for use in genotyping applications
  • Automated procedure saves time and effort
  • No organic extraction or alcohol precipitation

The BioSprint 96 DNA Plant Kit provides automated purification of total DNA from plant tissue on the BioSprint 96 using proven MagAttract magnetic particle technology. The kit contains all reagents and plasticware necessary for automated DNA purification.

Cat No./ID: 941557
BioSprint 96 DNA Plant Kit (576)
€864.00
Order Product
For 576 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents
Cat No./ID: 941558
BioSprint 96 DNA Plant Kit (1536)
€1,982.00
Order Product
For 1536 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents
The BioSprint 96 DNA Plant Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
1
Reproducible purification of high-quality DNA.
Genomic DNA was purified from 50 mg samples of fresh leaf tissue from [A] arabidopsis, [B] maize, and [C] rose. Purification was performed using the BioSprint 96 DNA Plant Kit and BioSprint 96 DNA Plant protocol. Purified DNA was eluted in 200 µl Buffer AE. Eluates (5 µl) were visualized on a 0.8% agarose gel. M: markers (1 kb ladder).
2

High PCR performance of purified DNA.

Genomic DNA was purified from 50 mg samples of fresh leaf tissue from [A] maize, [B] arabidopsis, and [C] rose using the BioSprint 96 DNA Plant Kit and BioSprint 96 DNA Plant protocol. DNA was eluted in 200 µl Buffer AE. Amplification (20 µl reaction volume) was carried out using the trnL PCR system (amplifying an intergenic region of a tRNA coding gene in the chloroplast genome) with 5 µl, 0.5 µl, or 0.05 µl purified DNA from 2 maize samples and 3 arabidopsis samples and with 0.5 µl or 0.05 µl purified DNA from 3 rose samples. M: markers (1 kb ladder).
Performance

The BioSprint 96 DNA Plant Kit enables purification of DNA from a wide range of plant species (see table).

DNA purified from a wide range of plant species
Sample typeAmount Yield (µg)
Arabidopsis, fresh leaves 50–120 mg 13–41
Cotton, fresh leaves 50 mg 10–26
Maize, fresh leaves 50 mg 10–41
Pine, fresh needles 100–190 mg 5–18
Rose, fresh new leaves 100–140 mg 40–100
Rose, fresh old leaves 100–140 mg 11–51
Soybean, fresh leaves 50 mg 39–80
Spruce, fresh needles 130–160 mg 5–13
Wheat, fresh leaves 50 mg 8–49
Maize, lyophilized leaves 5–10 mg 10–15.5
Rapeseed, lyophilized leaves 5–10 mg 13.5–23
Sunflower, lyophilized leaves 5–10 mg 57–93
Maize, dried seed powder 20–55 mg 9.8–33.5
Apple 1–6 seeds 5–30
French bean 1 bean 13–55
Grape 3 seeds 18.2–18.8
Linen 1–10 seeds 1.5–38
Lupine 2–7 seeds 18–97
Orange 1 seed 1–35
Rapeseed 1–4 seeds 8.5–47.5
Soybean 1 seed 80–105
Swedish turnip 5–50 seeds 21.5–52
Tomato 15–30 seeds 12–16
Turnip radish 4–18 seeds 11–41
Wheat 3–4 seeds 85–122
 
Purified DNA is up to 40 kb in size (see figure "Reproducible purification of high-quality DNA"). The DNA is ready to use in a wide range of downstream applications, including PCR and real-time PCR, genotyping, and microsatellite analysis (see figure "High PCR performance of DNA purified using the BioSprint 96 DNA Plant Kit").

Principle
The BioSprint 96 DNA Plant Kit is used with the BioSprint 96. The kit uses magnetic-particle technology for rapid DNA purification from plant material. The BioSprint 96 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure
The BioSprint 96 DNA Plant Kit enables purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen, or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed, and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to an S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart "BioSprint DNA Plant procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
Applications

The BioSprint 96 DNA Plant Kit purifies DNA for use in a range of downstream applications, including:

PCR
Real-time PCR
Genotyping
Microsatellite analysis
Features
Specifications
Applications PCR, real-time PCR, genotyping
Elution volume 200 µl
For automated processing BioSprint 96 workstation
Format 5-tube strip, S-Block
Main sample type Plant samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 10–100 mg
Technology Magnetic particles
Time per run or per prep 15 minutes
Yield 5–20µg

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