What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays?
FAQ ID - 3427

Pan Bacteria 1 and Pan Bacteria 3 are designed to detect the broadest possible collection of bacteria involved in human biology. We began by designing assays against evolutionarily conserved regions of the 16S rRNA gene. Bioinformatically these two assays are predicted to test positive for thousands of species, based off of perfect matches and near-perfect matches of the primers and probes with the target sequences. In order to confirm this on the bench, we assayed genomic DNA from 119 species. In the case of both assays, nearly all of the species showed amplification.  Positive tests were recorded for species within these 7 phyla: Actinobacteria, Bacteroidetes, Euryarchaeota, Firmicutes, Fusobacteria, Proteobacteria, and Tenericutes. Additionally, Pan Bacteria 3 detected species within the phylum Spirochaetes.

For a finer breakdown of what our bench testing covered:

 

Pan Bacteria 1

Phylum

Class

Order

Family

Actinobacteria

Actinobacteria

Actinomycetales

Alcaligenaceae

Bacteroidetes

Alphaproteobacteria

Bacillales

Bacillaceae

Euryarchaeota

Bacilli

Bacteroidales

Bacteroidaceae

Firmicutes

Bacteroidia

Bifidobacteriales

Bartonellaceae

Fusobacteria

Betaproteobacteria

Burkholderiales

Bifidobacteriaceae

Proteobacteria

Clostridia

Campylobacterales

Brucellaceae

Tenericutes

Deltaproteobacteria

Caulobacterales

Burkholderiaceae

 

Epsilonproteobacteria

Clostridiales

Campylobacteraceae

 

Flavobacteria

Coriobacteriales

Caulobacteraceae

 

Fusobacteriia

Desulfovibrionales

Clostridiaceae

 

Gammaproteobacteria

Enterobacteriales

Coriobacteriaceae

 

Methanobacteria

Flavobacteriales

Corynebacteriaceae

 

Mollicutes

Fusobacteriales

Desulfovibrionaceae

 

 

Lactobacillales

Enterobacteriaceae

 

 

Legionellales

Enterococcaceae

 

 

Methanobacteriales

Flavobacteriaceae

 

 

Mycoplasmatales

Fusobacteriaceae

 

 

Neisseriales

Helicobacteraceae

 

 

Pasteurellales

Lactobacillaceae

 

 

Pseudomonadales

Legionellaceae

 

 

Rhizobiales

Listeriaceae

 

 

Vibrionales

Methanobacteriaceae

 

 

Xanthomonadales

Micrococcaceae

 

 

 

Moraxellaceae

 

 

 

Mycobacteriaceae

 

 

 

Neisseriaceae

 

 

 

Pasteurellaceae

 

 

 

Peptostreptococcaceae

 

 

 

Porphyromonadaceae

 

 

 

Prevotellaceae

 

 

 

Pseudomonadaceae

 

 

 

Staphylococcaceae

 

 

 

Streptococcaceae

 

 

 

Veillonellaceae

 

 

 

Vibrionaceae

 

 

 

Xanthomonadaceae

Pan Bacteria 3

Phylum

Class

Order

Family

Actinobacteria

Actinobacteria

Actinomycetales

Alcaligenaceae

Bacteroidetes

Alphaproteobacteria

Bacillales

Bacillaceae

Euryarchaeota

Bacilli

Bacteroidales

Bacteroidaceae

Firmicutes

Bacteroidia

Bifidobacteriales

Bartonellaceae

Fusobacteria

Betaproteobacteria

Burkholderiales

Bifidobacteriaceae

Proteobacteria

Clostridia

Campylobacterales

Brucellaceae

Spirochaetes

Deltaproteobacteria

Caulobacterales

Burkholderiaceae

Tenericutes

Epsilonproteobacteria

Clostridiales

Campylobacteraceae

 

Flavobacteria

Coriobacteriales

Caulobacteraceae

 

Fusobacteriia

Desulfovibrionales

Clostridiaceae

 

Gammaproteobacteria

Enterobacteriales

Clostridiales

 

Methanobacteria

Flavobacteriales

Coriobacteriaceae

 

Mollicutes

Fusobacteriales

Corynebacteriaceae

 

Spirochaetes

Lactobacillales

Desulfovibrionaceae

 

 

Legionellales

Enterobacteriaceae

 

 

Methanobacteriales

Enterococcaceae

 

 

Mycoplasmatales

Flavobacteriaceae

 

 

Neisseriales

Fusobacteriaceae

 

 

Pasteurellales

Helicobacteraceae

 

 

Pseudomonadales

Lactobacillaceae

 

 

Rhizobiales

Legionellaceae

 

 

Spirochaetales

Listeriaceae

 

 

Vibrionales

Methanobacteriaceae

 

 

Xanthomonadales

Micrococcaceae

 

 

 

Moraxellaceae

 

 

 

Mycobacteriaceae

 

 

 

Neisseriaceae

 

 

 

Pasteurellaceae

 

 

 

Peptostreptococcaceae

 

 

 

Porphyromonadaceae

 

 

 

Prevotellaceae

 

 

 

Pseudomonadaceae

 

 

 

Spirochaetaceae

 

 

 

Staphylococcaceae

 

 

 

Streptococcaceae

 

 

 

Veillonellaceae

 

 

 

Vibrionaceae

 

 

 

Xanthomonadaceae