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Cignal Lenti Reporter Assays

For sensitive assessment of cell signaling activities in virtually any mammalian cell type

  • Transduce virtually any mammalian cell type
  • Transduce nondividing cells, stem cells, and differentiated cells
  • Use for transient experiments or develop stable cell line reporters
  • Transduction-ready lentivirus requires no further preparation

Cignal Lenti Reporter Assays are ready-to-transduce lentiviral particles for assessing cell signaling activities in virtually any mammalian cell type.

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Cignal Lenti Reporter Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


0
Determination of pathway activity in human primary cells.
The Cignal Lenti NFAT Reporter Assay was used to determine PKA/Ca2+ pathway activity. Cignal Lenti NFAT Reporter Assay (luc) (4 x 105 TU) and Cignal Lenti Renilla Control (luc) (1 x 105 TU) cotransduced around 10,000 normal human pulmonary artery smooth muscle cells (PASMC). After 48 hours transduction, medium was changed to assay medium. After 54 hours transduction, cells were treated with 10 ng/ml PMA and 0.5 µM ionomycin for 18 hours. Dual-luciferase assay was performed, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.
1
Notch signaling activity in rat glioma cells.
The Cignal Lenti RBP-Jk Reporter Assay was used to measure Notch signaling activity in C6 (rat glioma) cells. Cignal Lenti RBP-Jk Reporter Assay (luc) (2 x 105 TU) and Cignal Lenti Renilla Control (luc; 2 x 104 TU) transduced around 20,000 C6 cells. After 24 hours transduction, medium was changed to complete medium. After 48 hours transduction, cells were infected with 100 MOI of recombinant adenoviruses expressing constitutive active Notch1 (Ad-NICD) or 100 MOI of recombinant adenovirus expressing GFP (Ad-GFP). Dual-luciferase assay was performed 18 hours after infection, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.
2

Measurement of NFκB pathway activity.

The Cignal Lenti NFκB Reporter Assay was used to measure NFκB pathway activity in thymocytic cells (D1; Murine T-cell leukemia cells). Cignal Lenti NFκB Reporter Assay (luc) (2.5 x 105 TU) transduced around 10,000 D1 cells. After 48 hours transduction, cells were treated with 20 ng/ml recombinant mouse TNFα (hTNFα) protein for 18 hours. Luciferase assay was performed and promoter activity values are expressed as arbitrary units. Experiments were performed in triplicate and the standard deviation is shown.
3
Advantage of using dual luciferase system.
Cignal Lenti HIF-1 Reporter (luc) (1.5 x 105 TU) transduced around 15,000 HepG2 cells (24 hours before transduction 7,500 cells were plated per well of 96-well plate) with and without Cignal Lenti Renilla control (3 x 104 TU). After 42 hours of transduction, medium was changed to assay medium (Opti-MEM + 0.5% FBS + 0.1mM NEAA + 1mM Sodium pyruvate + 100 U/ml penicillin + 100 µg/ml streptomycin). After 48 hours of transduction, cells were treated with 300 µM of CoCl2 for 18 hours. Luciferase or dual-luciferase assay was performed, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were done in triplicates, and the standard deviation is indicated. The utilization of the dual-luciferase system (cotransduction of Cignal Lenti Renilla Control along with Cignal Lenti Reporter Assay (luc)) improves the fold induction change in hypoxia signaling activity, as normalization of firefly luciferase activity with Renilla luciferase activity corrects for cell death caused by the treatment.
4
Stable cell line generation
The Cignal Lenti NFkB Reporter Assay (luc) was used to generate an NFkB pathway sensor cell line for the study of the NFkB signal transduction pathway. The NFkB sensor cell line was developed by transduction of HEK-293 cells with the Cignal Lenti NFkB Reporter Assay (luc), followed by selection of a clonal population that maintained stable chromosomal integration of the lentiviral vector provirus and responded strongly to stimuli known to activate the NFkB pathway. The generation of stable HEK-293 NFkB sensor cell line was confirmed by testing the cell line with 10 ng/ml of recombinant hTNFα after 1, 5, 10, and 15 passages of the cell line. Stimulation of the NFkB pathway by hTNFα results in up to a 100-fold increase in expression of the reporter gene even after 2 months of cell culture.
5
Cignal Lenti Reporter Assay procedure.
6
Cignal Lenti Reporter Assay.
7
Cignal Lenti Reporter Assays.
Performance
Cignal Lenti Reporter Assays are highly suited for use in studies of cell signaling in primary cells, stem cells, and difficult-to-transfect cell lines (see figures "Determination of pathway activity in human primary cells", "Notch signaling activity in rat glioma cells", "Measurement of NFkB pathway activity" and "Advantage of using dual luciferase system"). They may also be used in the generation of stable cell lines (see figure "Stable cell line generation") and in high-throughput screening of siRNAs.
Principle

Cignal Lenti Reporter Assays utilize a unique combination of transcription factor reporter technology coupled with lentiviral delivery. Cignal Lenti Reporter Assays consist of multiple repeats of a specific transcription factor’s binding site and basic promoter elements to drive the expression of a reporter gene (firefly luciferase or GFP).

Lentiviruses are one of the most effective vehicles to introduce reporter constructs in almost all mammalian cells — including nondividing cells. A transduced lentiviralreporter construct is integrated into cellular genomic DNA and provides stable, long-term expression of a reporter gene.

These reporters are powerful tools in functional genomics and drug discovery for assessing pathway activity. When the pathway is activated or inhibited by a drug candidate, gene knockdown (using siRNA), overexpression event (expression vectors), or peptide, luciferase or GFP reporter activity is modulated and can be measured quantitatively and rapidly.

Safety

The Cignal Lenti Reporter Assays are ready-to-transduce, replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles (see figure "Cignal Lenti Reporter Assay" and table "Features of Cignal Lenti Reporter Assays"). The Cignal lentiviral particles are safe to use. It is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms. Follow all published RGL-2 guidelines for handling and waste decontamination. Details on the requirements for creating a BSL-2 work environment are available in the U.S. Department of Health and Human Services publication Biosafety in Microbiological and Biomedical Laboratories, 4th edition.

The biosafety features engineered into these vectors include the following: 

A deletion in the promoter/enhancer region of the U3 portion of 3' LTR that ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of target cells 
The Cignal Lenti Reporter Assay and plasmids express packaging proteins contain no significant areas of homology, minimizing any chance for recombination 
None of the HIV-1 genes (gag, pol, rev) are expressed in transduced cells, since they are expressed from packaging plasmids lacking packaging signal. Therefore, the lentiviral particles generated are replication-incompetent 
No virulence genes (vpr, vif, vpu, and nef) are present in the Cignal Lenti Reporter Assay

 

Features of Cignal Lenti Reporter Assays
FeatureFunction
RSV-5' LTR: Hybrid Rous Sarcoma Virus (RSV) enhancer/promoter-U5 long terminal repeat Permits viral packaging and reverse transcription of viral mRNA
Psi: Packaging signal Allows viral packaging
RRE: Rev response element Involved in packaging of viral transcript
Cppt: Central polypurine tract Involved in nuclear translocation and integration of transduced viral genome
Reporter gene (firefly luciferase or GFP) Allows quantification of transcription
hPGK: human phosphoglycerate kinase eukaryotic promoter Permits high-level expression of the mammalian selection marker (puromycin)
PuroR: puromycin resistance gene Can be used for mammalian selection
SIN/3' LTR: 3' self-inactivating long terminal repeat Modified 3' LTR that allows viral packaging but self-inactivates the 5' LTR for biosafety reasons, the element also contains a polyadenylation signal for efficient transcription termination
f1 ori: f1 origin of replication Allows episomal replication of plasmid in eukaryotic cells
AmpR: ampicillin resistance gene Allows selection of the plasmid in E. coli
TRE: Transcription response element Permits regulation of reporter gene expression by a specific transcription factor
TATA box Acts as an minimal promoter

Procedure

Cignal Lenti Reporter Assays are immediately ready for transduction, without the need to further generate or propagate lentivirus. These vectors are highly suited for transient transduction studies in difficult to transfect cells or for stable, pathway sensor cell line generation.

Transient pathway regulation studies in difficult-to-transfect cell lines

Target cells are transduced with the Cignal Lenti Reporter Assay. The cells are typically cultured for 24–48 hours to ensure lentivirus integration. The cultures are then treated with the biological agents of interest (siRNA, shRNA, chemical compound, viral expression vector, protein, or peptide). Reporter assays (firefly luciferase or GFP) are carried out 18–36 hours post-treatment, depending upon the treatment conditions (see flowchart "Cignal Lenti Reporter Assay procedure").

Stable pathway sensor cell line generation

Target cells are transduced with the Cignal Lenti Reporter Assay. Following transduction, the cells are cultured with puromycin selection to generate a homogenous population of transduced cells. If necessary, single-cell cloning may be carried out in order to isolate a clonal pathway sensor cell line. These pathway sensor cell lines serve as a valuable cell-based assay platform, for subsequent screening and mechanism of action studies.

Applications

Cignal Lenti Reporter Assays are powerful tools in functional genomics and drug discovery for assessing cell signaling activities in virtually any mammalian cell type. They are highly suited for assessing the biological impact of siRNAs, proteins, and small molecule compounds.

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Brochures & Guides (1)
For measurement of signaling pathways in any mammalian cell
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Kit Handbooks (1)
For lentiviral-based cell signaling activity assays
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References
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FOXO Reporter Assay (luc) Cignal Lenti Reporter Assays
Introduction
The Cignal Lenti Reporter Assays are self-inactivating replication incompetent viral particles. The lentiviral particles are ready to add directly to your cells. The Cignal Lenti FOXO Reporter is designed to monitor the transcriptional activity of FOXO in cultured cells. The FOXO-responsive luciferase vector encodes the firefly luciferase reporter gene under the control of a minimal (m)CMV promoter and tandem repeats of the FOXO binding site. We have experimentally optimized the number of response elements as well as the intervening sequence between response elements to maximize the signal to noise ratio. Using a simple luciferase assay, you can easily monitor the activity of PI3K/AKT signaling pathways and determine the effect of various treatments, such as gene knockdown, over-expression, and chemical compounds on those pathways. We recommend using the Cignal Lenti Positive Control (GFP) in your initial experiment, in order to establish the optimal multiplicity of infection (MOI) for the cell type being studied.
CLS-8022L

The Cignal Lenti FOXO Reporter Assay shows FOXO transcription activity. The Cignal Lenti FOXO Reporter (luc) [5 x 105 TU] and Cignal Lenti Renilla control [5 x 104 TU] transduced approximately 2 x 104 HEK-293H cells (24 hours before transduction, 104 cells were plated per well of a 96-well plate). After 24 hours of transduction, medium was changed to complete medium. After 30 hours of transduction, cells were infected with 10 MOI of recombinant adenovirus expressing FOXO3A (Ad-FOXO3A) or 10 MOI of recombinant adenovirus expressing GFP (Ad-GFP). A dual luciferase assay was performed 72 hours after transduction, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate, and the standard deviation is indicated.
Related Biologies
PI3K-AKT Signaling
Name Cignal Lenti FOXO Reporter (luc) (CLS-8022L)
Official symbol Zeb2 [Mouse]
Official name zinc finger E-box binding homeobox 2
Species Mouse (Mus musculus)
Entrez Gene ID 24136
Transcript(s) for this gene NM_001033635 (4312 bp), NM_015753 (9235 bp), ENSMUST00000145529, NM_001289521 (8898 bp), XM_006498042 (8765 bp), XM_006498043 (8588 bp), XM_006498044 (8613 bp), XM_006498045 (8821 bp), XM_006498046 (8768 bp), XM_006498047 (8587 bp), XM_011239102 (8717 bp)
Official symbol Ywhaq [Mouse]
Official name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Species Mouse (Mus musculus)
Entrez Gene ID 22630
Transcript(s) for this gene NM_011739 (2110 bp), ENSMUST00000146206
Official symbol Ins2 [Mouse]
Official name insulin II
Species Mouse (Mus musculus)
Entrez Gene ID 16334
Transcript(s) for this gene NM_001185083 (473 bp), NM_001185084 (587 bp), NM_008387 (485 bp)
Official symbol Cdkn1b [Mouse]
Official name cyclin-dependent kinase inhibitor 1B
Species Mouse (Mus musculus)
Entrez Gene ID 12576
Transcript(s) for this gene NM_009875 (2405 bp), XM_006505466 (4611 bp)
Official symbol Cyr61 [Mouse]
Official name cysteine rich protein 61
Species Mouse (Mus musculus)
Entrez Gene ID 16007
Transcript(s) for this gene NM_010516 (2028 bp)
Official symbol Abl1 [Mouse]
Official name c-abl oncogene 1, non-receptor tyrosine kinase
Species Mouse (Mus musculus)
Entrez Gene ID 11350
Transcript(s) for this gene NM_001112703 (7162 bp), NM_009594 (5933 bp), XM_130089 (5778 bp), ENSMUST00000156736, NM_001283045 (6039 bp), NM_001283046 (7367 bp), NM_001283047 (7306 bp), XM_006497621 (3779 bp), XM_011239012 (4950 bp)
Official symbol Hoxb6 [Mouse]
Official name homeobox B6
Species Mouse (Mus musculus)
Entrez Gene ID 15414
Transcript(s) for this gene XM_006532292 (1700 bp), NM_008269 (1344 bp)
Official symbol Ar [Mouse]
Official name androgen receptor
Species Mouse (Mus musculus)
Entrez Gene ID 11835
Transcript(s) for this gene NM_013476 (10063 bp)
Official symbol Esr1 [Mouse]
Official name estrogen receptor 1 (alpha)
Species Mouse (Mus musculus)
Entrez Gene ID 13982
Transcript(s) for this gene NM_007956 (6248 bp), XM_985634 (3048 bp), XM_985672 (6199 bp), XM_985704 (6760 bp), ENSMUST00000149195, XM_006512432 (6377 bp), XM_006512433 (6250 bp), XM_006512434 (6173 bp), XM_006512435 (6123 bp), XM_006512436 (2916 bp), NM_001302531 (6218 bp), NM_001302532 (6358 bp), NM_001302533 (2730 bp), XM_011243066 (6188 bp), XM_011243067 (6189 bp), XM_011243068 (6158 bp), XM_011243069 (1346 bp)
Official symbol Mmp3 [Mouse]
Official name matrix metallopeptidase 3
Species Mouse (Mus musculus)
Entrez Gene ID 17392
Transcript(s) for this gene NM_010809 (1801 bp)
Official symbol Akt1 [Mouse]
Official name thymoma viral proto-oncogene 1
Species Mouse (Mus musculus)
Entrez Gene ID 11651
Transcript(s) for this gene NM_001165894 (2657 bp), NM_009652 (2707 bp), ENSMUST00000159815, XM_006515415 (2642 bp), XM_006515416 (2489 bp), XM_006515417 (1584 bp)
Official symbol Igfbp1 [Mouse]
Official name insulin-like growth factor binding protein 1
Species Mouse (Mus musculus)
Entrez Gene ID 16006
Transcript(s) for this gene NM_008341 (1522 bp)
Official symbol Foxo1 [Mouse]
Official name forkhead box O1
Species Mouse (Mus musculus)
Entrez Gene ID 56458
Transcript(s) for this gene NM_019739 (5552 bp)
Official symbol Runx3 [Mouse]
Official name runt related transcription factor 3
Species Mouse (Mus musculus)
Entrez Gene ID 12399
Transcript(s) for this gene ENSMUST00000156478, XM_006538506 (3373 bp), XM_006537058 (3373 bp), NM_019732 (3891 bp)
Official symbol Smad1 [Mouse]
Official name SMAD family member 1
Species Mouse (Mus musculus)
Entrez Gene ID 17125
Transcript(s) for this gene NM_008539 (3133 bp), XM_006530746 (3054 bp)
Official symbol Eif4g1 [Mouse]
Official name eukaryotic translation initiation factor 4, gamma 1
Species Mouse (Mus musculus)
Entrez Gene ID 208643
Transcript(s) for this gene NM_001005331 (5460 bp), NM_145941 (5469 bp), ENSMUST00000154594, ENSMUST00000128840, XM_006521935 (5506 bp), XM_006521936 (5449 bp), XM_006521937 (5503 bp), XM_006521938 (5485 bp), XM_006521939 (5428 bp), XM_006521940 (5481 bp), XM_006521941 (5256 bp), XM_006521942 (5455 bp), XM_006521943 (5412 bp), XM_006521944 (5390 bp), XM_006521945 (5325 bp), XM_006521946 (5247 bp), NM_001304432 (5427 bp), XM_011245861 (5476 bp)
Official symbol Ppargc1a [Mouse]
Official name peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
Species Mouse (Mus musculus)
Entrez Gene ID 19017
Transcript(s) for this gene NM_008904 (6464 bp), NR_027710 (6495 bp), XM_006503774 (6852 bp), XM_006503775 (6368 bp), XM_006503776 (6415 bp), XM_006503777 (6417 bp), XM_006503778 (6652 bp), XM_006503779 (1542 bp)
Official symbol Crebbp [Mouse]
Official name CREB binding protein
Species Mouse (Mus musculus)
Entrez Gene ID 12914
Transcript(s) for this gene NM_001025432 (7507 bp), XM_148699 (5867 bp), XM_489496 (2805 bp), XM_489497 (4498 bp), XM_006521751 (10301 bp), XM_006521752 (10286 bp), XM_006521753 (10187 bp), XM_006521754 (10157 bp)
Official symbol Opn1mw [Mouse]
Official name opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan)
Species Mouse (Mus musculus)
Entrez Gene ID 14539
Transcript(s) for this gene NM_008106 (1221 bp), ENSMUST00000145443, ENSMUST00000123401, XM_006527824 (701 bp)
Official symbol Foxo6 [Mouse]
Official name forkhead box O6
Species Mouse (Mus musculus)
Entrez Gene ID 329934
Transcript(s) for this gene NM_194060 (2528 bp), XM_977851 (2376 bp), XM_987577 (2193 bp)
Official symbol Foxo3 [Mouse]
Official name forkhead box O3
Species Mouse (Mus musculus)
Entrez Gene ID 56484
Transcript(s) for this gene NM_019740 (2889 bp), XM_001000298 (2722 bp), XM_006512806 (6838 bp)
Official symbol Ccnd2 [Mouse]
Official name cyclin D2
Species Mouse (Mus musculus)
Entrez Gene ID 12444
Transcript(s) for this gene NM_009829 (5772 bp), XM_006505457 (6223 bp), XM_006505458 (6205 bp), XM_006505459 (6266 bp), XM_006505460 (6097 bp)
Official symbol Angpt2 [Mouse]
Official name angiopoietin 2
Species Mouse (Mus musculus)
Entrez Gene ID 11601
Transcript(s) for this gene NM_007426 (3560 bp), ENSMUST00000170200
Official symbol Sirt1 [Mouse]
Official name sirtuin 1
Species Mouse (Mus musculus)
Entrez Gene ID 93759
Transcript(s) for this gene NM_001159589 (3793 bp), NM_001159590 (3400 bp), NM_019812 (3910 bp), XM_975350 (3877 bp), ENSMUST00000148516, XM_006514342 (3460 bp), XM_006514343 (1135 bp), XM_011243606 (3697 bp)
Official symbol Foxo4 [Mouse]
Official name forkhead box O4
Species Mouse (Mus musculus)
Entrez Gene ID 54601
Transcript(s) for this gene ENSMUST00000150558, XM_006528139 (2617 bp), XM_983722 (1795 bp), XM_983678 (782 bp), XM_983128 (1796 bp), NM_018789 (3162 bp)
Official symbol Bcl2l11 [Mouse]
Official name BCL2-like 11 (apoptosis facilitator)
Species Mouse (Mus musculus)
Entrez Gene ID 12125
Transcript(s) for this gene NM_009754 (4782 bp), NM_207680 (5040 bp), NM_207681 (4872 bp), ENSMUST00000146023, NM_001284410 (2327 bp), NM_001291016 (2304 bp), XM_006498614 (5097 bp), XM_006498615 (6575 bp), XM_006498616 (4929 bp), XM_006498617 (4839 bp)
Official symbol Hmga2 [Mouse]
Official name high mobility group AT-hook 2
Species Mouse (Mus musculus)
Entrez Gene ID 15364
Transcript(s) for this gene NM_010441 (4226 bp), NM_178057 (3122 bp), ENSMUST00000159310, XM_006513251 (3869 bp), XM_011243374 (927 bp)
Official symbol Bcl2l11 [Rat]
Official name BCL2-like 11 (apoptosis facilitator)
Species Rat (Rattus norvegicus)
Entrez Gene ID 64547
Transcript(s) for this gene NM_022612 (423 bp), NM_171988 (5091 bp), NM_171989 (333 bp), XM_006234983 (5209 bp), XM_006234985 (5041 bp), XM_006234987 (4951 bp)
Official symbol Foxo3 [Rat]
Official name forkhead box O3
Species Rat (Rattus norvegicus)
Entrez Gene ID 294515
Transcript(s) for this gene NM_001106395 (2885 bp), XM_001069121 (2238 bp), XM_215421 (2238 bp)
Official symbol Hmga2 [Rat]
Official name high mobility group AT-hook 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 84017
Transcript(s) for this gene NM_032070 (802 bp)
Official symbol Foxo4 [Rat]
Official name forkhead box O4
Species Rat (Rattus norvegicus)
Entrez Gene ID 302415
Transcript(s) for this gene NM_001106943 (3159 bp), XM_001069832 (2337 bp), XM_228556 (2336 bp)
Official symbol Ppargc1a [Rat]
Official name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Species Rat (Rattus norvegicus)
Entrez Gene ID 83516
Transcript(s) for this gene NM_031347 (2440 bp), XM_008770219 (6376 bp), XM_008770220 (7546 bp)
Official symbol Crebbp [Rat]
Official name CREB binding protein
Species Rat (Rattus norvegicus)
Entrez Gene ID 54244
Transcript(s) for this gene NM_133381 (10103 bp), XM_008767524 (7157 bp)
Official symbol Runx3 [Rat]
Official name runt-related transcription factor 3
Species Rat (Rattus norvegicus)
Entrez Gene ID 156726
Transcript(s) for this gene NM_130425 (1230 bp), XM_001069039 (1251 bp), XM_001069092 (1272 bp)
Official symbol Eif4g1 [Rat]
Official name eukaryotic translation initiation factor 4 gamma, 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 287986
Transcript(s) for this gene XM_001060756 (5388 bp), XM_002724743 (5302 bp), XM_002727923 (5388 bp), XM_213569 (5409 bp), XM_003752527 (5409 bp), XM_006221194 (5449 bp), XM_006221195 (5379 bp), XM_006248581 (5285 bp), XM_006248582 (5274 bp), XM_006248583 (5265 bp), XM_006248584 (5352 bp), XM_006248585 (5293 bp), XM_008758181 (5457 bp), XM_008758184 (5400 bp), XM_008758186 (5454 bp), XM_008758188 (5445 bp), XM_008758195 (5446 bp), XM_008768835 (5349 bp)
Official symbol Foxo1 [Rat]
Official name forkhead box O1
Species Rat (Rattus norvegicus)
Entrez Gene ID 84482
Transcript(s) for this gene NM_001191846 (2560 bp), XM_001056726 (2560 bp), XM_342244 (2560 bp)
Official symbol Smad1 [Rat]
Official name SMAD family member 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 25671
Transcript(s) for this gene NM_013130 (3010 bp), XM_006255394 (3129 bp), XM_006255395 (3788 bp), XM_006255396 (3019 bp), XM_006255397 (3108 bp)
Official symbol Angpt2 [Rat]
Official name angiopoietin 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 89805
Transcript(s) for this gene NM_134454 (2296 bp), XM_001065522 (2470 bp), XM_344544 (2470 bp)
Official symbol Ccnd2 [Rat]
Official name cyclin D2
Species Rat (Rattus norvegicus)
Entrez Gene ID 64033
Transcript(s) for this gene NM_022267 (1415 bp)
Official symbol Sirt1 [Rat]
Official name sirtuin 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 309757
Transcript(s) for this gene NM_001107627 (640 bp), XM_001080493 (3994 bp), XM_228146 (3508 bp), XM_003751934 (3787 bp), XM_003753475 (3850 bp), XM_006223877 (3701 bp), XM_006256146 (3698 bp), XM_008772947 (3126 bp), XM_008774950 (3129 bp), XM_008774951 (3093 bp)
Official symbol Akt1 [Rat]
Official name AKT serine/threonine kinase 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 24185
Transcript(s) for this gene NM_033230 (1613 bp), XM_006240631 (2838 bp), XM_008764918 (4335 bp)
Official symbol Igfbp1 [Rat]
Official name insulin-like growth factor binding protein 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 25685
Transcript(s) for this gene NM_013144 (1500 bp), XM_001053362 (1333 bp)
Official symbol Esr1 [Rat]
Official name estrogen receptor 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 24890
Transcript(s) for this gene NM_012689 (2090 bp), XM_006227832 (6792 bp), XM_008758703 (6840 bp), XM_008758704 (6764 bp)
Official symbol Mmp3 [Rat]
Official name matrix metallopeptidase 3
Species Rat (Rattus norvegicus)
Entrez Gene ID 171045
Transcript(s) for this gene NM_133523 (1827 bp)
Official symbol Ar [Rat]
Official name androgen receptor
Species Rat (Rattus norvegicus)
Entrez Gene ID 24208
Transcript(s) for this gene NM_012502 (4137 bp)
Official symbol Abl1 [Rat]
Official name ABL proto-oncogene 1, non-receptor tyrosine kinase
Species Rat (Rattus norvegicus)
Entrez Gene ID 311860
Transcript(s) for this gene NM_001100850 (6158 bp), XM_001067860 (6158 bp), XM_001079831 (6158 bp), XM_231137 (4904 bp), XM_347217 (3953 bp), XM_006233918 (4950 bp)
Official symbol Hoxb6 [Rat]
Official name homeo box B6
Species Rat (Rattus norvegicus)
Entrez Gene ID 497986
Transcript(s) for this gene XM_001081290 (1957 bp), XM_573182 (1971 bp), XM_003752371 (1595 bp), XM_006247232 (1818 bp)
Official symbol Cyr61 [Rat]
Official name cysteine-rich, angiogenic inducer, 61
Species Rat (Rattus norvegicus)
Entrez Gene ID 83476
Transcript(s) for this gene NM_031327 (1987 bp)
Official symbol Cdkn1b [Rat]
Official name cyclin-dependent kinase inhibitor 1B
Species Rat (Rattus norvegicus)
Entrez Gene ID 83571
Transcript(s) for this gene NM_031762 (1755 bp), XM_006237539 (2403 bp)
Official symbol Ywhaq [Rat]
Official name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Species Rat (Rattus norvegicus)
Entrez Gene ID 25577
Transcript(s) for this gene NM_013053 (2099 bp)
Official symbol Zeb2 [Rat]
Official name zinc finger E-box binding homeobox 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 311071
Transcript(s) for this gene NM_001033701 (3648 bp), XM_242005 (3300 bp), XM_006234144 (9064 bp), XM_006234145 (9584 bp), XM_006234147 (9781 bp), XM_008761747 (8830 bp), XM_008761748 (5707 bp)
Official symbol YWHAQ [Human]
Official name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Species Human (Homo sapiens)
Entrez Gene ID 10971
Transcript(s) for this gene NM_006826 (2272 bp), ENST00000474715, ENST00000238081, ENST00000381844
Official symbol INS [Human]
Official name insulin
Species Human (Homo sapiens)
Entrez Gene ID 3630
Transcript(s) for this gene NM_000207 (469 bp), NM_001185097 (495 bp), NM_001185098 (648 bp), NM_001291897 (529 bp), ENST00000250971, ENST00000381330, ENST00000397262, ENST00000512523
Official symbol CYR61 [Human]
Official name cysteine-rich, angiogenic inducer, 61
Species Human (Homo sapiens)
Entrez Gene ID 3491
Transcript(s) for this gene NM_001554 (2295 bp), ENST00000480413, ENST00000451137, ENST00000617517
Official symbol ZEB2 [Human]
Official name zinc finger E-box binding homeobox 2
Species Human (Homo sapiens)
Entrez Gene ID 9839
Transcript(s) for this gene NM_001171653 (9171 bp), NM_014795 (9243 bp), NR_033258 (3026 bp), XM_006712881 (8658 bp), XM_006712882 (8740 bp), ENST00000303660, ENST00000409487, ENST00000419938, ENST00000440875, ENST00000462355, ENST00000465070, ENST00000470879, ENST00000539609, ENST00000558170, ENST00000627532, ENST00000629520
Official symbol ABL1 [Human]
Official name ABL proto-oncogene 1, non-receptor tyrosine kinase
Species Human (Homo sapiens)
Entrez Gene ID 25
Transcript(s) for this gene NM_005157 (5596 bp), NM_007313 (5881 bp), ENST00000318560
Official symbol HOXB6 [Human]
Official name homeobox B6
Species Human (Homo sapiens)
Entrez Gene ID 3216
Transcript(s) for this gene NM_018952 (1686 bp), NM_156036 (2704 bp), NM_156037 (1657 bp), ENST00000490419, XM_005257283 (3560 bp), XM_005257284 (3616 bp), XM_005257285 (2798 bp), XM_006721856 (1672 bp), ENST00000225648, ENST00000484302
Official symbol CDKN1B [Human]
Official name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Species Human (Homo sapiens)
Entrez Gene ID 1027
Transcript(s) for this gene NM_004064 (2535 bp), ENST00000477087, ENST00000228872, ENST00000396340
Official symbol ESR1 [Human]
Official name estrogen receptor 1
Species Human (Homo sapiens)
Entrez Gene ID 2099
Transcript(s) for this gene NM_000125 (6330 bp), NM_001122740 (6357 bp), NM_001122741 (6314 bp), NM_001122742 (6466 bp), ENST00000488573, NM_001291230 (6320 bp), NM_001291241 (6311 bp), XM_006715374 (6282 bp), XM_006715375 (5809 bp), ENST00000206249, ENST00000338799, ENST00000406599, ENST00000427531, ENST00000440973, ENST00000443427, ENST00000456483
Official symbol MMP3 [Human]
Official name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Species Human (Homo sapiens)
Entrez Gene ID 4314
Transcript(s) for this gene NM_002422 (1828 bp), ENST00000299855
Official symbol FOXO1 [Human]
Official name forkhead box O1
Species Human (Homo sapiens)
Entrez Gene ID 2308
Transcript(s) for this gene NM_002015 (5738 bp), ENST00000473775, ENST00000379561
Official symbol IGFBP1 [Human]
Official name insulin-like growth factor binding protein 1
Species Human (Homo sapiens)
Entrez Gene ID 3484
Transcript(s) for this gene NM_000596 (1660 bp), NM_001013029 (1606 bp), ENST00000275525, ENST00000457280, ENST00000468955
Official symbol AKT1 [Human]
Official name v-akt murine thymoma viral oncogene homolog 1
Species Human (Homo sapiens)
Entrez Gene ID 207
Transcript(s) for this gene NM_001014431 (2794 bp), NM_001014432 (2878 bp), NM_005163 (3008 bp), ENST00000557552, XM_005267401 (2924 bp), ENST00000349310, ENST00000402615, ENST00000407796, ENST00000544168, ENST00000554581, ENST00000554848, ENST00000555528
Official symbol SMAD1 [Human]
Official name SMAD family member 1
Species Human (Homo sapiens)
Entrez Gene ID 4086
Transcript(s) for this gene NM_001003688 (2880 bp), NM_005900 (3056 bp), ENST00000515527, XM_005262991 (2186 bp), XM_005262992 (2044 bp), XM_005262993 (1827 bp), XM_006714217 (2095 bp), ENST00000302085, ENST00000394092, ENST00000515385
Official symbol RUNX3 [Human]
Official name runt related transcription factor 3
Species Human (Homo sapiens)
Entrez Gene ID 864
Transcript(s) for this gene NM_001031680 (4340 bp), NM_004350 (4270 bp), ENST00000496967, XM_005246024 (4041 bp), XM_006711016 (4097 bp), ENST00000308873, ENST00000338888, ENST00000399916, NM_001320672 (4173 bp), XM_011542351 (4175 bp)
Official symbol EIF4G1 [Human]
Official name eukaryotic translation initiation factor 4 gamma, 1
Species Human (Homo sapiens)
Entrez Gene ID 1981
Transcript(s) for this gene NM_001194946 (5559 bp), NM_001194947 (5502 bp), NM_004953 (5050 bp), NM_182917 (5686 bp), NM_198241 (5538 bp), NM_198242 (5123 bp), NM_198244 (5300 bp), ENST00000493299, NM_001291157 (5581 bp), ENST00000319274, ENST00000342981, ENST00000346169, ENST00000350481, ENST00000352767, ENST00000382330, ENST00000392537, ENST00000411531, ENST00000414031, ENST00000424196, ENST00000427845, ENST00000434061, ENST00000435046, ENST00000441154
Official symbol CREBBP [Human]
Official name CREB binding protein
Species Human (Homo sapiens)
Entrez Gene ID 1387
Transcript(s) for this gene NM_001079846 (10083 bp), NM_004380 (10197 bp), ENST00000576720, XM_005255124 (9999 bp), XM_005255125 (9627 bp), XM_006720848 (9783 bp), ENST00000262367, ENST00000382070
Official symbol PPARGC1A [Human]
Official name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Species Human (Homo sapiens)
Entrez Gene ID 10891
Transcript(s) for this gene NM_013261 (6318 bp), ENST00000515534, XM_005248130 (6312 bp), XM_005248131 (6496 bp), XM_005248132 (6240 bp), XM_005248134 (5978 bp), ENST00000264867, ENST00000507380, ENST00000612355, ENST00000613098, ENST00000617484
Official symbol OPN1LW [Human]
Official name opsin 1 (cone pigments), long-wave-sensitive
Species Human (Homo sapiens)
Entrez Gene ID 5956
Transcript(s) for this gene NM_020061 (1277 bp), ENST00000463296, ENST00000369951
Official symbol CCND2 [Human]
Official name cyclin D2
Species Human (Homo sapiens)
Entrez Gene ID 894
Transcript(s) for this gene NM_001759 (6531 bp), ENST00000541542, XM_005253813 (1076 bp), ENST00000261254
Official symbol FOXO3 [Human]
Official name forkhead box O3
Species Human (Homo sapiens)
Entrez Gene ID 2309
Transcript(s) for this gene NM_001455 (7341 bp), NM_201559 (7314 bp), XM_005266867 (6495 bp), XM_005266868 (6587 bp), ENST00000343882, ENST00000406360, ENST00000540898
Official symbol BCL2L11 [Human]
Official name BCL2 like 11
Species Human (Homo sapiens)
Entrez Gene ID 10018
Transcript(s) for this gene NM_001204106 (4836 bp), NM_001204107 (5033 bp), NM_001204108 (5238 bp), NM_001204109 (5127 bp), NM_001204110 (4968 bp), NM_001204111 (4822 bp), NM_001204112 (4947 bp), NM_001204113 (958 bp), NM_006538 (4926 bp), NM_138621 (5106 bp), NM_138622 (5231 bp), NM_138623 (5051 bp), NM_138624 (5139 bp), NM_138625 (4952 bp), NM_138626 (5002 bp), NM_138627 (4732 bp), NM_207002 (728 bp), NM_207003 (4961 bp), XM_005263550 (1647 bp), XM_005263551 (5151 bp), XM_005263552 (1467 bp), XM_005263553 (4971 bp), XM_005263554 (5047 bp), XM_005263555 (1377 bp), XM_005263556 (1594 bp), XM_005263557 (1652 bp), XM_005263559 (4881 bp), XM_005263560 (4867 bp), XM_005263561 (1414 bp), ENST00000308659, ENST00000337565, ENST00000357757, ENST00000393256, ENST00000405953, ENST00000610735, ENST00000615946, ENST00000619294, ENST00000620862, ENST00000621302, ENST00000622509, ENST00000622612, XM_011510461 (954 bp), XM_011510462 (833 bp), XM_011510463 (1892 bp), XM_011510464 (774 bp), XM_011510465 (2304 bp)
Official symbol AR [Human]
Official name androgen receptor
Species Human (Homo sapiens)
Entrez Gene ID 367
Transcript(s) for this gene NM_000044 (10661 bp), NM_001011645 (8112 bp), ENST00000513847, ENST00000374690, ENST00000396043, ENST00000396044, ENST00000504326, ENST00000612010, ENST00000612452, ENST00000613054
Official symbol FOXO4 [Human]
Official name forkhead box O4
Species Human (Homo sapiens)
Entrez Gene ID 4303
Transcript(s) for this gene NM_001170931 (3200 bp), NM_005938 (3365 bp), ENST00000466874, ENST00000341558, ENST00000374259
Official symbol SIRT1 [Human]
Official name sirtuin 1
Species Human (Homo sapiens)
Entrez Gene ID 23411
Transcript(s) for this gene NM_001142498 (3604 bp), NM_012238 (4110 bp), ENST00000497639, XM_005269663 (3295 bp), XM_006717737 (3935 bp), ENST00000212015, ENST00000403579, ENST00000406900, ENST00000432464
Official symbol ANGPT2 [Human]
Official name angiopoietin 2
Species Human (Homo sapiens)
Entrez Gene ID 285
Transcript(s) for this gene NM_001118887 (5267 bp), NM_001118888 (5114 bp), NM_001147 (5270 bp), ENST00000325203, ENST00000338312, ENST00000523120, ENST00000629816
Official symbol HMGA2 [Human]
Official name high mobility group AT-hook 2
Species Human (Homo sapiens)
Entrez Gene ID 8091
Transcript(s) for this gene NM_001015886 (4298 bp), NM_003483 (4150 bp), NM_003484 (1539 bp), ENST00000545998, NM_001300918 (1274 bp), NM_001300919 (1878 bp), ENST00000354636, ENST00000393577, ENST00000393578, ENST00000403681, ENST00000425208, ENST00000536545, ENST00000541363

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