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DNeasy PowerSoil Pro Kit

For the isolation of microbial genomic DNA from all soil types

Features

  • Efficient lysis of bacteria and fungi in all soil types
  • Up to 8-fold higher DNA yields compared to alternative methods
  • Recovery of inhibitor-free DNA for direct use in NGS applications
  • Unbiased results accurately represent sample alpha diversity
  • Automation on the QIAcube

Useful Resources

DNeasy PowerSoil Pro Kit Handbook DNeasy PowerSoil Pro Kit Quick-start Protocol Exploring the temperate leaf microbiome: From natural forests to controlled experiments and urban environments
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DNeasy PowerSoil Pro Kit (250)

Cat. No. / ID: 47016

For the isolation of microbial genomic DNA from all soil types.
 €1,870.00
Preparations
250
50
The DNeasy PowerSoil Pro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Extracting microbial DNA from soil samples can be challenging. QIAGEN’s new DNeasy PowerSoil Pro Kit is even more effective than our original PowerSoil technology at isolating high yields of pure microbial DNA from all soil types, including compost, clay and top soil. The kit features a novel bead tube and optimized chemistry for more efficient lysis of soil bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate the challenging inhibitors commonly found in soil and environmental samples in even less time. Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods. Extraction of DNA using the DNeasy PowerSoil Pro Kit can be automated on the QIAcube Connect.
Want to try this solution for the first time? Request a quote for a trial kit.>

Performance

Significantly increased DNA yields from challenging samples

The DNeasy PowerSoil Pro Kit includes a novel bead tube and improved lysis chemistry, which enables isolation of up to 8-fold higher yields of DNA compared to the first generation DNeasy PowerSoil Kit and those of competitors in all soil types tested (See: ' Isolate more high-quality genomic DNA.'). Both the DNeasy PowerSoil Kit and the new DNeasy PowerSoil Pro Kit isolated high-quality DNA from challenging soil types (See: ' Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.').
Unbiased identification of total diversity and community representation in soil samples

DNA isolated using the DNeasy PowerSoil Pro Kit identified more bacteria (OTUs) in soil samples when compared with the DNeasy PowerSoil Kit and those of two competitors (See: ' Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.').
See figures
12888-Figure201112888-Figure201112888-Figure202112888-Figure202112888-Figure202112888-Figure2021

Principle

Inhibitor Removal Technology increases purity of isolated DNA

The DNeasy PowerSoil Pro Kit features a streamlined Inhibitor Removal Technology (IRT) to decrease sample processing time. In a comparison of methods for DNA isolation from soil, the DNeasy PowerSoil Pro Kit was the only method showing 260/280 ratios near 1.8 for all soil types, as well as the highest 260/230 ratios, indicating the absence of inhibitors. Samples isolated using the kit also showed no inhibition in PCR, in contrast to competitor methods (See: ' Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.').
See figures
12888-Figure202112888-Figure2021

Procedure

The soil or stool samples are lysed via chemical and mechanical homogenization. Lysis buffer is added to a mixed zirconium bead tube containing the sample. Bead beating can be carried out using a standard benchtop vortex with bead tube adapter or the high-powered PowerLyser. Crude lysate is then subjected to inhibitor removal for cleanup. Following the cleanup, purified lysate is mixed with an equal volume of DNA binding solution and passed through a silica spin filter membrane. The membrane is washed with a two-step washing regime. Silica-bound DNA is then eluted using a 10 mM Tris elution buffer. Relevant downstream applications for the isolated DNA include PCR, NGS and enzymatic digestion assays.

Supporting data and figures

Figure 1: Isolate more high-quality genomic DNA.

250 mg of the three different soil types were prepared using commercially available sample preparation solutions and compared to the new DNeasy PowerSoil Pro Kit. The DNeasy PowerSoil Pro Kit out-performed other available kits, resulting in 8-fold greater recovery of DNA across the variety of soil types.
Figure 1: Isolate more high-quality genomic DNA.
Figure 1: Isolate more high-quality genomic DNA.
Figure 3: Increased bacterial OTUs with the new DNeasy PowerSoil Pro Kit.
Figure 2: Improved, high-quality DNA yields with the new DNeasy PowerSoil Pro Kit.
Figure 4: Increased purity and less variability in DNA extracted with the DNeasy PowerSoil Pro Kit.

Resources

Webinars (3)
Exploring the temperate leaf microbiome: From natural forests to controlled experiments and urban environments
The work presented here thus offers an original assessment of the dynamics at play in the tree phyllosphere.
Characterizing the microbiome of neonates and infants to explore associations with health and disease
This webinar will focus on the acquisition and development of the preterm gut microbiome from birth and following discharge from intensive care.
Automated nucleic acid purification from diverse sample types using dedicated microbiome kits on the QIAcube
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome.
Application Notes (2)
Novel procedure for improved results in soil microbiome analysis using the DNeasy PowerSoil Pro Kit
PDF (345KB)
Extraction of microbiome DNA using the DNeasy PowerSoil Pro Kit manually and automated on the QIAcube Connect and QIAcube
PDF (819KB)
Brochures & Guides (2)
DNeasy PowerSoil Pro Kit Digital Product Profile
PDF (332KB)
DNeasy PowerSoil Pro Kit Product Profile
PDF (1MB)
Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Quick-Start Protocols (1)
DNeasy PowerSoil Pro Kit Quick-start Protocol
PDF (507KB)
Kit Handbooks (1)
DNeasy PowerSoil Pro Kit Handbook
PDF (1MB)

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