Tenfold dilutions of human leukocyte RNA (100 ng to 1 ng) were used as template in real-time one-step RT-PCR. Triplicate reactions were run using a TaqMan Gene Expression Assay for IL12RB1 (interleukin 12 receptor, beta 1). Greater sensitivity (i.e., lower CT values) was achieved with [A] the Rotor-Gene Q and Rotor-Gene Probe RT-PCR Kit than with [B] an instrument and kit from Supplier AII.
