PCR/qPCR/dPCR
PCR sensible, especÃfica, robusta y fiable
El éxito de los experimentos de amplificación génica depende de varios factores crÃticos, como el rendimiento, la sensibilidad del ensayo, la robustez de la reacción incluso con moldes difÃciles, y la normalización fiable de los datos. También puede ser necesario superar los retos especÃficos asociados al método de PCR que se utilice.
Elija el método que elija, ofrecemos numerosas soluciones optimizadas para PCR convencional, qPCR y dPCR. Nuestros kits, ensayos, instrumentos y accesorios le ayudarán a amplificar y detectar dianas con confianza y a optimizar el flujo de trabajo de prácticamente cualquier aplicación.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Examinar PCR/qPCR/dPCR
Productos
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134 productos encontrados
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity Digital PCR System
Para aplicaciones de PCR digital basadas en nanoplaca
QIAcuity OneStep Advanced Probe Kit
Para la RT-PCR de un paso en los instrumentos de PCR digital QIAcuity.
QuantiTect PCR Kits
Para la qPCR y la qRT-PCR de dos pasos mediante SYBR Green I y para la qPCR y la qRT-PCR de dos pasos, asà como la qPCR múltiple y la qRT-PCR de dos pasos y múlt. con sondas especÃficas de secuencia.
QuantiTect RT-PCR Kits
Para la qRT-PCR de un paso mediante SYBR® Green I y qRT-PCR de un paso y qRT-PCR múltiple mediante sondas especÃficas de secuencia para el análisis de la expresión génica
QuantiNova PCR Kits
Para PCR y PCR múlt. en tiempo real bas. en sondas ultrarrápidas y de gran sensib. y espec. y para ob. de result. inmejorables con qPCR bas. en SYBR Green.
miRCURY LNA miRNA PCR Assays para PCR digital
Para cuantificación sumamente sensible y especÃfica del miARN mediante dPCR basada en QIAcuity EvaGreen
QuantiNova RT-PCR Kits
Para la qRT-PCR multiple y qRT-PCR de un paso de alto nivel de sensibilidad y especificidad con sondas concretas de secuencia o para la qPCR con SYBR® Green I para el analisis de expresion genica
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
dPCR Microbial DNA Detection Assays
Para detección por dPCR de dianas microbianas: genes bacterianos, fúngicos, parasitarios, vÃricos, resistentes a antibióticos o de factores de virulencia
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QIAcuity High Multiplex Probe PCR Kit
Para muestras desafiantes y multiplexado de dPCR de más de cinco dianas
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
QIAcuity Cell and Gene Therapy (CGT) dPCR Assays
Para la valoración del genoma del vector y la determinación del número de copias del vector con precisión, reproducibilidad y velocidad superiores en el QIAcuity Digital PCR System
QIAcuity Residual DNA Quantification and Sizing Kits
For quantification and sizing of host cell DNA with higher accuracy and precision using the QIAcuity Digital PCR System
QIAcuity OneStep Advanced EG Kit
For dye-based one-step RT-PCR on the QIAcuity digital PCR instruments
miRCURY LNA miRNA Custom PCR Assays para PCR digital
Para cuantificación sumamente sensible y especÃfica del miARN mediante dPCR basada en QIAcuity EvaGreen
Q-Solution Kit
Este kit incluye Q-Solution y MgCl2 para obtener mejores resultados de las reacciones de PCR con configuraciones más difÃciles.
QIAcuity Mycoplasma Quant Kit and Standards
For digital PCR quantification and detection of mycoplasma contamination using the QIAcuity Digital PCR System
QIAcuity UCP Probe PCR Kit
Kits de Ultra Clean Production para su uso con los instrumentos de PCR digital QIAcuity
dPCR Copy Number Variation (CNV) Probe Assays
Para el análisis especÃfico de los locus de las variaciones en el número de copias y las alteraciones mediante dPCR simple o múltiple.
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
VeraSeqâ„¢ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
QIAcuity mericon Food Testing Kits
For digital PCR authentication of food and animal feed using QIAcuity
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
VeraSeqâ„¢ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeqâ„¢ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
QIAcuity Nanoplates y accesorios
Para uso con los instrumentos de PCR digital QIAcuity
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
QIAcuity Probe PCR Kit
Para uso con los instrumentos de PCR digital QIAcuity
QuantiTect Reverse Transcription Kit
For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
REPLI-g Single Cell Kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Rotor-Gene Q
For outstanding performance in real-time PCR
Rotor-Gene Q (CA)
For outstanding performance in real-time PCR
QIAquant
For fast and high performance real-time PCR in a 96- or 384-well format
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
QuantiFast Pathogen +IC Kits
Para la detección sensible y fiable de ARN/ADN vÃrico y ADN bacteriano, incluido el control interno
miRCURY LNA RT Kit
Para una sÃntesis óptima de ADNc de primera hebra con los miRCURY LNA miRNA PCR Systems utilizando SYBR Green o sonda de hidrólisis
miRCURY LNA miRNA PCR Assays
Para una cuantificación sumamente sensible y especÃfica de miARN con PCR de miARN optimizada con LNA y basada en SYBR® Green
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
QuantiNova Pathogen +IC Kit
Para la detección ultrarrápida y simultánea de ADN y ARN vÃrico y ADN bacteriano, incluido el control interno
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QIAcuity EG PCR Kit
Para uso con los instrumentos de PCR digital QIAcuity
QuantiTect Primer Assays
For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
QuantiTect Virus Kits
Para la detección muy sensible de ARN y/o ADN vÃrico
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
REPLI-g Advanced DNA Single Cell Kit
For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA
Sensiscript RT Kit
For reverse transcription of less than 50 ng RNA per reaction for end-point PCR
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
miRCURY LNA miRNA Custom PCR Panels
Placas personalizadas de 96 y 384 pocillos de ensayos de qPCR de miARN mejorados con LNA y basados en SYBR® Green
Certal Residual DNA Detection Kits
Para la deteccion sensible basada en sondas de ADN residual de celulas huesped de ovario de hamster chino (CHO), incluido un control interno
dPCR LNA Mutation Assays
Para detectar mutaciones de secuencias de ADN especÃficas relacionadas con el cáncer y la oncogénesis
miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
Taq PCR Master Mix Kit
Taq PCR Master Mix Kit is a 2x solution of Taq DNA polymerase, dNTPs, MgCl2 and PCR buffer to simplify your workflow, reduce contamination risk and improve throughput and reproducibility
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
QuantiFast Multiplex PCR Kits
For fast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
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