PyroMark Q96 Systems

For reagents and support of discontinued instrument PyroMark Q96 ID

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PyroMark MTHFR (96)

Cat no. / ID. 972432

For detection of variants in the MTHFR gene, 8 x 24 reactions on PyroMark Q24

AssayAccessoriesSoftwareKit

Assays and Tests

Buffers

Consumables

PyroMark Software

PyroMark Kits

Type

Q24

GeneGlobe CpG Assay

GeneGlobe Custom Assay

Detection of

MTHFR

HFE

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✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Consider upgrading to the PyroMark Q48 Autoprep System for these advantages

Automation of the Pyrosequencing protocol
Longer read lengths with the Advanced chemistry
Compact instrument size
Enabled for connectivity
Touch screen display that guides users through the process

Product Details

The PyroMark Q96 MD has been discontinued and is no longer supported.

The PyroMark Q96 ID was discontinued in 2016 and the instrument is no longer actively sold. Existing instruments will be supported until the end of August 2025. Reagents will continue to be available until the end of August 2025.

To future-proof your Pyrosequencing assays, consider upgrading your work to using a PyroMark Q48 Autoprep System, which is actively sold and supported by QIAGEN.

Service Plans

Pyro Q96 ID, Preventive Subscription

Cat no. / ID. 9243548A

One on-site Preventive Maintenance or Inspection Service visit for the PyroMark Q96 ID System, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

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Resources

Download Safety Data Sheets for QIAGEN product components.

Scientific poster

A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.

パイロシークエンス（Pyrosequencing）解析用に至適化された感度と精度の高い1 ステップRT-PCR

For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems

For use with PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems

FAQ

The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.

FAQ-2827

PyroMark Gold Q96 Reagents:

PyroMark Gold Q96 SQA Reagents (1 x 96) should be used for performing SQA analyses on the PyroMark Q96 ID. It can also be used to supplement the PyroMark Gold Q96 Reagents (5 x 96) when long runs lead to a shortage of nucleotides.
PyroMark Gold Q96 Reagents (5 x 96) should be used for SNP and CpG analyses on the PyroMark Q96 ID and MD instruments. On the PyroMark Q96 MD, the kit contains enough for 15x96 plates.
PyroMark Gold Q96 CDT Reagents (6 x 96) should be used only with CDTs on the PyroMark Q96 MD instrument, for performing SNP and CpG anslyses. The nucleotides are pre-diluted for use with CDTs, so this is not to be used on ID instrument or with NDTs on the MD.
PyroMark Gold Q96 Reagents (50 x 96) should be used only with the PyroMark Q96 MDA (automated option) for SNP anslyses. Not for use with the PyroMark Q96 ID or non-automated MD.

See the PyroMark Gold Q96 Reagents Handbook for additional information.

FAQ-2836

The PyroMark Q96 MD uses the NDT and CDT to dispense nucleotides, while the Q24 and Q96 ID use cartridges.

NDT means nucleotide dispensing tip. This is the original dispensing tip which is more sensitive to dust but dispenses lower nucleotide volumes and enables longer read length.

CDT means caplillary dispensing tip and is comparable to Q24 and Q96 ID cartridges. They dispense a higher volume but are easier in handling.

The NDT and CDT use two different tip holders which cannot be interchanged

FAQ-2841

Reconstiuted enzyme and substrate of PyroMark Gold Reagents, should not be vortexed since this could lead to conformational changes which affect the activity.

FAQ-2844

The PyroMark Q96 ID software version 1.0 contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries) and an SQA mode for de novo sequencing analysis. This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used.

FAQ-2845

The new PyroMark Assay Design Software Version 2.0 contains new algorithms to facilitate CpG assay design and enables PCR and sequencing primer design for all pyroseqeuncing applications and PyroMark instruments.

FAQ-2849

The PyroMark CpG LINE-1 assay is specific for human DNA and was not tested on mouse DNA. Mouse LINE-1 elements differ slightly in sequence and may not be detected.

FAQ-2859

The PyroMark Q96 MD HS Capillary Tip and HS Reagent Tip can be used up to 20 times and the PyroMark Q96 HS Nucleotide Tip up to 10 times.

FAQ-2865

The PyroMark Q96 MD software contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries). This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used. SQA analysis for de novo sequencing is not possible.

See our Product Selection Guide for additional information on CpG supplementary software.

FAQ-2866

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