The novel technology of the HPV sign Q96 and Q24 complete kits allow sequencing of a 30 base pair, hypervariable, genomic region of human papillomavirus (HPV). Both kits (Q96 and Q24) can be used in combination with the appropriate Pyrosequencing platform to detect and analyze HPV genotypes. Starting from a single amplification product, direct sequencing provides reliable identification of HPV genotypes.
The HPV sign kits include an HPV Library, which permits detection of low- and high-risk HPV types. Sample sequences are aligned against genotype-specific sequences in the HPV Library using IdentiFire software. This enables accurate detection of more than 100 HPV types, including all anogenital high- and low-risk types, with results expressed by a score number based on the homology to the reference sequences included in the HPV sign Library.