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HotStar HiFidelity Polymerase Kit

Read phase-out notice below
For highly sensitive and reliable high-fidelity hot-start PCR
  • High sensitivity and specificity
  • Unique UA/TA cloning feature
  • Fidelity 10-fold higher than Taq DNA Polymerase
  • Room-temperature setup and fast enzyme activation

This ready-to-use, optimized kit includes everything required for high-fidelity PCR — enzyme, buffers, and dNTPs. HotStar HiFidelity DNA Polymerase is a hot-start proofreading enzyme uniquely modified to produce A overhangs, enabling direct and streamlined UA/TA cloning. The buffer contains Factor SB to prevent degradation of primers and template during PCR setup, providing highly sensitive and reliable high-fidelity PCR. In addition, Q-Solution enables efficient amplification of "difficult" (e.g., GC rich) templates.


IMPORTANT NOTE: This product is phasing-out until October 15, 2020, and afterward, available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit.


For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.


Applications Cloning, mutation analysis, TA- or UA-cloning procedures
Enzyme activity 3' -> 5' exonuclease activity, A-addition
Mastermix No
Reaction type PCR amplification, proofreading
Real-time or endpoint Endpoint
Sample/target type Genomic DNA and cDNA
Single or multiplex Single
With/without hotstart With hotstart

Product Resources

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