Removing highly expressed, but biologically unimportant RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, removal of unwanted RNA species from full length and fragmented RNA samples can be particularly challenging and can result in suboptimal performance.
QIAseq FastSelect Epidemiology Kits are a pan-bacterial ribosomal RNA (rRNA) removal kit designed to remove 5S, 16S and 23S rRNA, and human, mouse and rat cytoplasmic and mitochondrial ribosomal RNA from complex samples. Our comprehensive rRNA removal reagent has been designed using 16S (nearly 600,000 entries), 23S (nearly 170,000 entries) and 5S rRNA sequences (over 7200 entries). In-silico modeling predicts >95% rRNA removal of all known 5S, 16S and 23S rRNA sequences.
QIAseq FastSelect Epidemiology Kits can accommodate RNA amounts ranging from as little as 20 ng up to 1 µg, with consistently high performance, and is compatible with low-quality, highly fragmented or high-quality full-length RNA. Since QIAseq FastSelect does not use enzymatic digestion or hybrid-capture procedures, the fast, simple workflow results in reliable rRNA removal and high reproducibility in downstream applications.
Our unique QIAseq FastSelect procedure is significantly faster than existing methods and QIAseq FastSelect Epidemiology Kits have been tested with QIAGEN, Illumina and NEB stranded RNA-seq library kits