QIAamp UltraSens Virus Kit

For concentration and purification of viral RNA and DNA from serum and plasma

Features

  • Rapid purification of high-quality, ready-to-use viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors
QIAamp UltraSens Virus Kit (50)

Cat. No. / ID: 53704

For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
Preparations
50
250
The QIAamp UltraSens Virus Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAamp UltraSens Virus Kit uses a novel technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. Starting with sample volumes of up to 1 ml, nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation.

Performance

The QIAamp UltraSens Virus Kit is suitable for purification of viral nucleic acids from a wide range of viral species. It can also be used for efficient isolation of extracellular genomic DNA from plasma and serum. The purified nucleic acids perform well in sensitive downstream applications (see figures "High performance in one-step PCR" and " High performance in RT-PCR").

The purified DNA and RNA can be used in a wide range of downstream applications, including:

  • PCR and quantitative real-time PCR
  • Infectious disease research
See figures

Principle

No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp technology yields viral RNA and DNA from cell-free body fluids that are ready to use in PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

Procedure

Nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation. This step allows nucleic acid purification from large sample volumes without the inconvenience of handling large volumes throughout the protocol. Viral nucleic acids are then purified using QIAamp silica-gel-membrane technology (see flowchart " Procedure"). Lysates are loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.

No ultracentrifugation or specialized laboratory equipment is required, and an internal control can be added at the start of the procedure, allowing full monitoring of the purification process.

See figures

Applications

The QIAamp UltraSens Virus Kit uses new technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. The procedure provides increased sensitivity in viral-load monitoring and other applications where high viral nucleic acid recovery is essential. Samples of 1 ml plasma or serum can be prepared within 1 hour, with typical yields of >85% recovery of nucleic acids and 60 µl elution volume.

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsPCR, qPCR, real-time PCR.
timeperrunorperprep1 hour
formatMinElute columns
elutionvolume60 µl
processingManual (centrifugation)
sampleamount1 ml
technologySilica technology
purificationoftotalrnamirnapolyamrnadnaorproteinViral DNA, viral RNA
mainsampletypeSerum, plasma
yield>85% recovery

Resources

Kit Handbooks (2)
For highly efficient purification of viral RNA and DNA from 1 ml plasma and serum samples
Quick-Start Protocols (1)
Brochures & Guides (2)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?

The exact composition of Buffer AC is confidential. This buffer is a proprietary component of QIAamp UltraSens Virus Kits. We do not sell Buffer AC separately.

FAQ ID - 3429
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
Yes, it is possible to isolate free genomic DNA from plasma and serum using the QIAamp UltraSens Virus Kit.
FAQ ID -358
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12