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artus Parvo B19 PCR Kits CE

For quantitative detection of parvovirus DNA using real-time PCR

Features

  • Compliance with EU IVD Directive 98/79/EC
  • High reliability using the internal control
  • Highly sensitive detection of as few as 0.2 IU/µl (RG kit)
  • Accurate quantitation using the 5 standards supplied

Products

artus Parvo B19 PCR Kits CE are intended for in vitro diagnostic use in Europe.
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artus Parvo B19 LC PCR Kit (96) CE

Cat. No. / ID: 4504065

For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 5 Quantitation Standards, Internal Control, Water (PCR grade)
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artus Parvo B19 RG PCR Kit (24) CE

Cat. No. / ID: 4504263

For 24 reactions on the Rotor-Gene 3000: Master, 5 Quantitation Standards, Internal Control, Water (PCR grade)
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artus Parvo B19 LC PCR Kit (24) CE

Cat. No. / ID: 4504063

For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, Mg Solution, 5 Quantitation Standards, Internal Control, Water (PCR grade)
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artus Parvo B19 TM PCR Kit (24) CE

Cat. No. / ID: 4504163

For 24 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, 5 Quantitation Standards, Internal Control, Water (PCR grade)

Product Details

artus Parvo B19 PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation for in vitro diagnostic use.

Performance

To ensure highest sensitivity, artus Parvo B19 PCR Kits have been optimized to detect low numbers of parvovirus B19 DNA. The analytical sensitivity of the artus Parvo B19 RG PCR Kit is 0.2 IU/µl in the PCR on the Rotor-Gene 3000.

Performance characteristics of artus Parvo B19 RG PCR Kit
Kitartus Parvo B19 RG PCR Kit
Validated sample types Serum or plasma
Analytical sensitivity 0.2 IU/µl in the PCR

Principle

artus Parvo B19 PCR Kits are based on the amplification and simultaneous detection of a specific region of the parvovirus B19 genome using real-time PCR. The kits provide high levels of specificity, sensitivity, and reproducibility.

Each artus Parvo B19 PCR Kit provides 5 quantitation standards (see figure " Reliable quantitation of parvovirus B19 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical parvovirus B19 PCR is not reduced.

Specifications of artus Parvo B19 RG PCR Kit
Kitartus Parvo B19 RG PCR Kit
Sample types  Serum or plasma
Amplicon  76 bp region of the parvovirus B19 genome

See figures

Procedure

artus Parvo B19 PCR Kits provide all necessary reagents optimized for reliable parvovirus B19 DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix (and Mg solution, LC kit only), and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit is to be used.

Applications

artus Parvo B19 PCR Kits enable the rapid and sensitive detection of parvovirus B19 DNA from serum and plasma with accurate quantification. The kits are available for use on the Rotor-Gene 3000, the LightCycler 1.1/1.2/1.5 Instruments, or the ABI PRISM 7000, 7700, 7900HT SDS.

Supporting data and figures

Specifications

FeaturesSpecifications
quantitativequalitativeQuantitative
sampletypeSerum, plasma
recommendedsampleprepQIAamp UltraSens Virus Kit
ruoceasrCE
whatdetectedParvovirus DNA
thermalcyclerABI PRISM 7000, 7700, 7900HT SDS, LightCycler, Rotor-Gene 3000