The EGFR Plus RGQ PCR Kit comprises four triplex assays, each containing all the oligonucleotides (primers, probes and oligonucleotide clamp, where applicable) necessary to amplify and detect the targeted EGFR mutations. Manual or automated genomic DNA (gDNA) extraction must first be performed from either FFPE tissue or plasma. When starting from FFPE tissue, manual gDNA extraction is performed with the QIAamp DSP DNA FFPE Tissue Kit (cat. no. 60404). When starting from plasma, cfDNA extraction is performed with the QIAamp DSP Circulating Nucleic Acid Kit (cat. no. 61504). In combination with the QIAsymphony SP instrument, automated gDNA extraction from FFPE samples is performed with the QIAsymphony DSP DNA Mini Kit (cat. no. 937236), while automated cfDNA extraction from plasma samples is performed with the QIAsymphony DSP Circulating DNA Kit (cat. no. 937556). Following gDNA extraction, the DNA is quantified followed by PCR on the Rotor-Gene Q instrument. After the PCR run, analysis and expression calls are performed manually.