ipsogen JAK2 MutaSearch Kit

For qualitative detection of the JAK2 V617F/G1849T mutation using real-time PCR

Features

  • Sensitive and accurate qualitative assay
  • Improved level of detection with 1% clinical positivity cut-off
  • Easy workflow with ready-to-use solutions

Useful Resources

ipsogen JAK2 MutaSearch Kit (24)

Cat. No. / ID: 673813

For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

The ipsogen JAK2 MutaSearch Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for sensitive and reliable detection and qualitative analysis of the JAK2 V617F/G1849T mutation in genomic DNA.

Performance

To ensure highest sensitivity, the ipsogen JAK2 MutaSearch Kit has been optimized to detect the JAK2 V617F/G1849T mutation and uses a DNA cut-off sample with 1% (COS) positivity and DNA positive and negative controls for qualitative analysis (see figures  Accurate detection of JAK2 V617F mutation, 1% cut-off sample, and positive and negative controls and  Reliable detection of wild-type JAK2, 1% cut-off sample, and positive and negative controls). A minimum of 25 ng of input sample DNA is recommended.
See figures

Principle

The ipsogen JAK2 MutaSearch Kit is a ready-to-use kit for the detection and qualitative analysis of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure  JAK2 mutation site identification). The kit provides high levels of sensitivity and reproducibility. The ipsogen JAK2 MutaSearch Kit provides a DNA cut-off sample (COS), a positive control (PC), and a negative control (NC) for JAK2 V617F. Use of the cut-off sample and controls enables accurate qualitative analysis of genotyping results.
See figures

Procedure

The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaSearch Kit provides sensitive detection and qualitative analysis of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen JAK2 MutaSearch Kits enable sensitive and reliable detection and qualitative analysis of the JAK2 V617F/G1849T mutation for research use only.

Supporting data and figures

Resources

Kit Handbooks (1)