HRM Technology - FAQs
Answers to your questions
Data analysis and interpretation
HRM technology applications
Find out what HRM can do for your research.
Q1. Can HRM be used for microsatellite analysis?
A: There are some published studies that show microsatellite HRM analysis data. It generally depends on the number of expected permutations and combinations, as HRM is a comparative analysis method and all variables and controls must be in place for an auto call.
Q2. Can multiplexing be performed using HRM?
A: Yes, to a limited extent. If amplicons melt at different temperatures, distinct melting profiles can be observed. HRM analysis of up to 4 small amplicons has been achieved.
Q3. Can predictive tests be performed using a normal melt and derivative plot?
A: This is only applicable to certain changes with large Tm differences and in certain amplicons. However, these are limiting as an A/T change in class IV SNPs cannot be detected using normal melt curve analysis, but is readily detected using HRM.