Universal total RNA was characterized using the TGFβ/BMP Signaling Pathway RT2 Profiler PCR Array, followed by dissociation (melting) curve analysis. The PCR array specifically detects individual genes, despite the expression of related gene-family members in the same RNA sample.
4 replicate sets of raw threshold data (1–4) obtained by 2 different scientists (A and B) at 2 different times using Human Drug Metabolism RT2 Profiler PCR Arrays were directly compared. The results demonstrate a high degree of correlation (R2 >0.990).
A representative set of assays for 4000 genes used in RT2 Profiler PCR Arrays have an average amplification efficiency of 99% with a 95% CI from 90–110%. Consistently high amplification efficiencies enable PCR arrays to accurately analyze multiple genes simultaneously utilizing the ΔΔCT method.
The RT2 lncRNA PCR Array system comprises a complete experimental workflow, from sample preparation through to data analysis and interpretation, starting with just 25 ng of RNA (or <1 ng of RNA with the RT2 PreAMP cDNA Synthesis Kit) and provides free data analysis software. Each cataloged RT2 lncRNA PCR Array incorporates laboratory-verified assays for 84 pathway focused genes, 5 housekeeping genes for normalization and controls that check for sample quality and reaction quality.
Different amounts of universal total RNA were characterized using the Human Inflammatory Cytokines and Receptors RT2 Profiler PCR Array and the percentage of detectable genes calculated for each amount. As little as 25 ng total RNA yields an >80% positive call, even for cytokines expressed at very low levels.