REPLI-g UltraFast Mini Kit

The availability of sufficient quantities of genomic DNA for genomic analysis is often lacking. Whole genome amplification overcomes this limitation by global amplification of all DNA within a sample, providing sufficient quantities to perform all analyses on the same DNA sample. REPLI-g UltraFast technology provides fast and highly uniform DNA amplification across the entire genome, with minimum sequence bias. The method is based on multiple displacement amplification (MDA) technology — isothermal genome amplification utilizing a new, uniquely processive DNA polymerase that gives DNA products of up to 100 kb in length in a much shorter time, while still avoiding the high sequence bias of PCR-based amplification methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and more reliable results in downstream assays.

REPLI-g amplified genomic DNA can be used in a variety of downstream applications, including:

• Genotyping (e.g., SNP, STR, microarray)
• End-point PCR, quantitative real-time PCR
• Sequencing 
• Comparative genome hybridization