QIAprep 96 Turbo Miniprep Kit
For purification of up to 20 µg molecular biology grade plasmid DNA per well
The QIAprep 96 Turbo Miniprep Kit provides QIAprep 96 and TurboFilter 96 plates for purification of up to 20 µg molecular biology grade plasmid DNA per well.
QIAprep 96 Turbo Miniprep Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The QIAprep 96 Turbo Miniprep Kit is designed for isolation of up to 20 µg pure plasmid or cosmid DNA for use in routine molecular biology applications, such as sequencing and cloning (see figure "High-quality DNA for automated sequencing").
In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.
QIAprep 96 plates (see figure "QIAvac 96 with 96-well plate") contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. Pure plasmid DNA eluted from QIAprep 96 plates is immediately ready to use — there is no need to precipitate, concentrate, or desalt.
TurboFilter 96 plates are specially designed to allow fast clearing of bacterial lysates generated under high concentrations of chaotropic salts. Crude bacterial lysates are vacuum filtered through the TurboFilter 96 plate, eliminating the need for centrifugation. Particle-free lysates flow directly into the wells of the QIAprep 96 plate, ready for purification and elution. TurboFilter technology streamlines QIAprep plasmid purification.
Plasmid purification using QIAprep 96 Turbo Miniprep Kits follows a simple bind–wash–elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by filtration through TurboFilter modules. The cleared lysates are then applied to the QIAprep 96 plate where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.
The QIAprep 96 Turbo procedure is performed entirely in 96-well format, reducing sample handling to a minimum, allowing 96 parallel minipreps to be processed in 45 minutes. Bacterial cultures can be grown and lysed in the 96-well blocks provided with the kit. The QIAvac 96 manifold is designed for in-line filtration in 96-well format, so that cleared lysates from the TurboFilter 96 plate flow directly into the wells of the QIAprep 96 plate, providing high-throughput of 96 samples in parallel.
QIAprep 96 Turbo Miniprep Kits provide reproducible yields of pure DNA suitable for use in many applications, including:
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