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QIAEX II Gel Extraction Kit

Solution과 agarose gels로 부터 DNA fragments (40 bp에서 50 kb까지)를 분리 정제할 수 있습니다
  • 40 bp부터 50 kb까지 DNA를 효율적으로 분리
  • TAE 혹은 TBE buffer로 만든 low-melt agarose gel 혹은 standrad gel과 polyacrylamide gel로 부터 gel extraction
  • Subsequent reactions에 영향을 주는 sodium iodide 없음
  • Large DNA fragments의 손상 없음
The QIAEX II Gel Extraction Kit provides a suspension of silica particles to which DNA fragments bind in the presence of chaotropic salts. QIAEX II Suspension is added to solutions or solubilized agarose gel slices and binds DNA. The particles are collected by a brief centrifugation, washed, and DNA from 40 bp to 50 kb is eluted in Tris buffer or water.
Cat No./ID: 20021
QIAEX II Gel Extraction Kit (150)
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For 150 extractions: 3 x 0.5 ml QIAEX II Suspension, Buffers
Cat No./ID: 20051
QIAEX II Gel Extraction Kit (500)
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For 500 extractions: 5 x 1.0 ml QIAEX II Suspension, Buffers
The QIAEX II Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAEX II procedure.
Consistent recovery.
Recovery of various amounts of a 2.9 kb DNA fragment from 1% agarose gels using the QIAEX II Gel Extraction Kit is shown.

Using the QIAEX II Gel Extraction Kit, 10 ng to 10 µg DNA are recovered efficiently (see figure "Consistent recovery"). The versatile batch procedure can be easily scaled up to a binding capacity of 15 µg using 30 µl QIAEX II suspension.

The QIAEX II Gel Extraction Kit provides silica particles for purifying 60–95% DNA fragments (40 bp – 50 kb). A volume of 10 µl QIAEX II suspension binds up to 5 µg DNA, which is subsequently eluted in 20 µl.

Recovery according to size
DNA sizeRecovery, percent*
44 bp 75
75 bp 75
500 bp 95
7.5 kb 85
23.5 kb 75
48.5 kb 60
* From 2 µg loaded on a 1% TAE agarose gel.

Purification of DNA fragments with the QIAEX II system is based on solubilization of agarose and selective adsorption of nucleic acids onto QIAEX II silica-gel particles in the presence of chaotropic salt. QIAEX II separates DNA from salts, agarose, polyacrylamide, dyes, proteins, and nucleotides without phenol extraction or ethanol precipitation. QIAEX II is effective for any type of agarose in either TAE or TBE buffers.

QIAEX II particles ensure efficient recovery without shearing, even for large DNA fragments. Optimized buffers permit DNA recovery without sodium iodide, which is difficult to remove from DNA samples, and may affect subsequent reactions.

The solubilization and binding buffer used with the QIAEX II system contains a unique pH indicator. A simple color change indicates whether the pH of the binding mixture is optimal for efficient adsorption of DNA to QIAEX II silica particles (see figure "pH indicator dye"). The colored dye also allows easy visualization of any unsolubilized agarose in the binding mixture, ensuring complete solubilization for maximum yield.


QIAEX II silica-gel particles are added to the solubilized gel slice, and the particles collected by a brief centrifugation step (see flowchart "QIAEX II procedure"). After washing, the pure DNA fragment is eluted in 20 µl of Tris buffer or water.

The QIAEX II Gel Extraction Kit provides QIAEX II suspension together with binding and wash buffers, and a comprehensive handbook. QIAEX II Suspension is also sold separately. Protocols are provided for purification of DNA from agarose gels, solutions, and polyacrylamide gels.


DNA purified with the QIAEX II system can be used directly in most applications, including:

Restriction digestion
Binding capacity 5 µg/10 µl
Elution volume 20 µl
Format Tube
Fragment size 40 bp – 50 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: dsDNA fragments
Removal <10mers 17–40mers dye terminator proteins Removal <40mers
Sample type: applications DNA: PCR reactions
Technology Silica technology

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