Plant cell, tissue 혹은 fungi에서 total cellular DNA를 30 ug까지 분리할 수 있습니다
Contaminants와 enzyme inhibitors 없는 순수한 DNA
Ready-to-use DNA의 신속한 분리 정제
Organic extraction, 또는 ethanol precipitation 불필요
The DNeasy Plant Mini Kit provides fast and easy silica-based DNA purification in convenient spin column format. Typical yields are 3–30 μg of high-quality DNA, depending on the samples used. Purification of DNA using the DNeasy Plant Mini Kit can be automated on the QIAcube.
250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)
The DNeasy Plant Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Pure DNA (20–25 kb) for restriction analysis.
Agarose gel (0.8%) analysis of DNA isolated from the indicated leaves or needles with the DNeasy Plant Mini Kit was perfromed. Undigested DNA (0.5 µg, left) or 1 µg of EcoRI-digested DNA (right) were loaded in each pair of lanes. M: lambda-HindIII.
The DNeasy Plant Mini Kit allows rapid and efficient isolation of high-quality DNA
from a wide variety of plant species and tissue types including the most demanding
sources (see table "Selection of plant species processed with DNeasy Kits"). Samples
may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates
the QIAshredder spin column, a unique filtration and homogenization column that
efficiently removes cell debris and improves sample handling following lysis.
Selection of plant species processed with DNeasy Kits
Abies alba (silver fir)
Nicotiana tabacum (tobacco)
Aesculus hippocastanum (horse chestnut)
Oryza sativa (rice)4
Arabidopsis thaliana (thale cress)
Pelargonium sp. (geranium)4
Avena sp. (oat)
Brassica napus (oilseed rape)
Pinus sylvestris (Scotch pine), P. brutia5
Brassica oleracea (kohlrabi)
Populus tremula (aspen)
Chicorium endivia (chicory)
Pseudotsuga menziesii (Douglas fir)
Citrullini lanatus (water melon)
Quercus robur, Q. petrea (oak)6,7
Fagus sylvatica (beech)1
Rubus idaeus (raspberry)
Helianthus spp. (sunflower)
Solanum tuberosum (potato)
Hordeum vulgare (barley)2
Sphagnum palustre (moss)
Humulus sp. (hops)
Spinacia oleracea (spinach)
Taxus baccata (yew)
Triticum aestivum (wheat)4
Ulmus glabra (elm)6
Lycopersicon esculentum (tomato)3
Vitis spp. (grape)6
Zea mays (maize)
Young leaves or needles (and other tissues, as indicated) were collected and immediately
flash frozen. DNA isolation was then performed with the DNeasy Plant Mini Kit. 1Beechnut, 2dried
leaves, 3callus, 4leaves from adult plant, 5endosperm,
6old leaves, rich in carbohydrates,
7buds. For more information on DNA isolation
from other species including fungi, call QIAGEN Technical Services or your local
The typical yield is 3–30 µg, with a sample size of up to 100 mg wet weight, and
an elution volume of 50–400 µl. The DNA yields vary between different species and
tissues depending on genome size, ploidy, cell number, and age of tissue sample.
Lower and higher range values correspond to arabidopsis and wheat, respectively.
The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and
other secondary metabolites often copurified using conventional methods. Such impurities
can interfere with spectrophotometric readings and inhibit enzymatic reactions.
Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm (see figures
purity from oak leaves" and "DNA
purity from pine needles"), confirming that the DNA is free of impurities,
including enzyme inhibitors. DNeasy purified DNA is sized up to 40 kb (see figure
DNA"). Purified DNA can be used in a wide range of applications (see figures
and "RAPD analysis").
DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary — purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.
Samples are first mechanically disrupted and then chemically lysed (see flowchart "DNeasy Plant and DNeasy 96 Plant procedures"). RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNEasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.
The DNeasy Plant Mini Kit provides purification of DNA from plant tissue, including:
PCR, real-time PCR, blotting
Main sample type
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.