TransMessenger Transfection Reagent
mRNA transfection 또는 plasmid DNA와 siRNA를 동시에 효율적으로 transfection할 수 있습니다
TransMessenger Transfection Reagent is a ready-to-use, lipid-based reagent for RNA transfection of eukaryotic cells. Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Cell lines successfully transfected with TransMessenger Reagent include 293T, Jurkat, and Vero. This product can also be used for RNA transfection of primary human fibroblasts, rat cardiomyocytes, and rat neuronal cells.
The TransMessenger Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
TransMessenger Reagent provides a fast and easy procedure and high transfection efficiencies (see figure "TransMessenger Reagent with HeLa cells"). Since the amount of RNA is a critical factor for successful transfection, we recommend optimizing the amounts of RNA and TransMessenger Transfection Reagent for every cell type–RNA combination (see figure "Amount of RNA and TransMessenger Reagent vs. transfection efficiency"). To facilitate this, the reagent is provided with guidelines for optimization together with starting points for optimization in different cell-culture formats.
TransMessenger Transfection Reagent is the first reagent specifically developed for transfection of cells with RNA. The reagent is a lipid-based formulation that is used in conjunction with a specific RNA-condensing enhancer and an optimized buffer. RNA molecules are condensed by the enhancer and then coated by TransMessenger Reagent for efficient transfer into eukaryotic cells. Strict quality control is performed to test for absence of RNase activity, lot-to-lot consistency, and low endotoxin levels (≤10 EU/ml). Our rigorous standards eliminate reagent variables that can adversely affect the efficiency of RNA transfection.
All TransMessenger Reagent components are provided as ready-to-use solutions. To generate TransMessenger–RNA transfection complexes, simply mix your RNA with Enhancer R and Buffer EC-R and incubate for 5 minutes at room temperature, then add TransMessenger Reagent and incubate for a further 5–10 minutes. The complexes are mixed with medium and added directly to the cells. Following a 3 hour incubation, the medium is changed and the cells are incubated until they are ready for analysis.
Optimal transfection results are achieved using high-purity RNA that is free of DNA, proteins, and other contaminants. RNA purified with RNeasy and Oligotex mRNA Kits is highly recommended.
Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Transfected RNA sequences are expressed in the absence of transcription, and in a promoter-independent manner. In addition, protein expression usually occurs sooner following transfection of RNA rather than DNA. RNA transfection with TransMessenger Transfection Reagent could be used for:
fragment fix placeholder