For low- to medium-throughput sample disruption for molecular analysis
- Simultaneous disruption of up to 12 samples
- Compact instrument with small footprint
- Coolable adapter to prevent biomolecule degradation
- Reproducible results with all sample types
- Compatible with all laboratory workflows
The TissueLyser LT is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time. This throughput matches that of the QIAcube, which automates sample preparation using trusted QIAGEN spin-column kits. Simultaneous disruption and homogenization is achieved through high-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. The TissueLyser LT must be used in combination with the coolable TissueLyser LT Adapter, which holds tubes during the disruption process. Additional accessories include beads and bead dispensers.
Explore the virtual demo to learn more about the TissueLyser LT.
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TissueLyser LT
Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
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85600
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TissueLyser LT Adapter, 12-Tube
Adapter for disruption of up to 12 samples in 2 ml microcentrifuge tubes on the TissueLyser LT
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69980
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TissueLyser LT适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。
高效的组织破碎。|从各种类型组织中纯化完整的蛋白,用于分析。|从动物组织中纯化高品质DNA。|从植物组织中纯化高品质DNA。|从植物组织中纯化完整的RNA。|纯化的RNA的RIN值高。|TissueLyser LT.|
使用TissueLyser LT或TissueLyser II破碎不同的大鼠组织。[A] 在QIAcube全自动核酸纯化仪上使用DNeasy Blood & Tissue Kit从25 mg样本中纯化DNA。利用分光光度计测定DNA产量。[B] 在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit (皮肤、心脏和肺) 或RNeasy Lipid Tissue Mini Kit (大脑),从20 mg样本中纯化RNA。利用分光光度计测定RNA产量。|将多只大鼠的组织(每份25 mg)稳定于Allprotect Tissue Reagent中,然后使用Tissuelyser LT或TissueLyser II破碎。使用Qproteome Mammalian Protein Prep Kit纯化样本中的总蛋白,之后通过Western印迹进行分析。[A]用Ponceau S染色染印膜,并且[B]进行Western印迹。LT:Tissuelyser LT;II:TissueLyser II ;M:分子量标准。|将多种大鼠组织(每份25 mg)稳定于Allprotect Tissue Reagent中,然后使用TissueLyser LT或TissueLyser II进行破碎。在QIAcube全自动核酸纯化仪上使用DNeasy Blood & Tissue Kit纯化DNA,然后使用HotStarTaq Plus Master Mix Kit和PGK1引物体系进行PCR反应。在QIAxcel上使用QIAxcel DNA High Resolution Kit分析120 bp的PCR产物。LT: TissueLyser LT; II: TissueLyser II; M: 分子量标准。|使用TissueLyser LT或TissueLyser II在预冷的适配器中破碎多种植物组织(每份100 mg)。在QIAcube全自动核酸纯化仪上使用DNeasy Plant Mini Kit纯化DNA,然后在琼脂糖凝胶上进行电泳分析。LT: TissueLyser LT; II: TissueLyser II; M: 分子量标准。|使用TissueLyser LT在预冷的适配器中破碎不同的植物组织 (各100 mg),重复两次。使用RNeasy Plant Mini Kit纯化RNA,然后进行琼脂糖凝胶电泳分析。清晰的核糖体RNA条带表示完整RNA的纯化。|使用TissueLyser LT破碎大鼠的心脏和肺 (各20 mg)。在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit纯化RNA,然后在Agilent 2100 Bioanalyzer上进行分析。RIN值分别为8.3 (心脏) 和9 (肺)。||
性能
The TissueLyser LT provides effective disruption of human, animal, and plant tissues, bacteria, and yeast, allowing reproducible yields of DNA, RNA, and protein in subsequent purification procedures (see figure "Effective tissue disruption"). Even difficult-to-lyse tissues such as heart and brain can be processed by the TissueLyser LT. DNA, RNA, and protein remain intact after disruption (see figures "Pure RNA with high RIN values", "Intact protein suitable for all types of analysis", "High-quality DNA from animal tissues", "High-quality DNA from plant tissues", and "Intact RNA from plant tissues"), enabling reliable analysis in downstream applications.
Disruption with the TissueLyser LT is comparable to that achieved with the well-established TissueLyser II (see figures "Effective tissue disruption", "Intact protein suitable for all types of analysis", "High-quality DNA from animal tissues", and "High-quality DNA from plant tissues").
原理
Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueLyser LT system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").
The TissueLyser LT is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser LT is fully compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems"), and also complements QIAGEN's range of automated solutions for medium-throughput sample preparation and analysis (see table "QIAGEN medium-throughput automation"). These include the QIAcube, which automates sample purification using QIAGEN spin-column kits, and the QIAxcel, which automates multicapillary gel electrophoresis of DNA and RNA.
| RNA |
Easy-to-lyse tissue |
RNeasy Kits |
| RNeasy Plus Kits |
| RNeasy Protect Kits |
| RNA |
Fiber-rich tissue |
RNeasy Fibrous Tissue Kits |
| RNA |
All types of tissue |
RNeasy Lipid Tissue Kits |
| RNeasy Universal Tissue Kits |
| RNA |
Plant tissue |
RNeasy Plant Mini Kit |
| RNA |
Yeast |
RNeasy Kits |
| RNA |
Bacteria |
RNeasy Protect Bacteria Kits |
| microRNA |
All types of tissue |
miRNeasy Kits |
| DNA |
Human tissue |
QIAamp DNA Kits |
| DNA |
Animal tissue |
DNeasy Blood & Tissue Kits |
| Protein |
Tissue |
Qproteome Mammalian Protein Prep Kit |
| Phosphoprotein |
Tissue |
PhosphoProtein Purification Kit |
| Glycoprotein |
Tissue |
Qproteome Glycoprotein Kits |
| DNA and RNA |
Tissue |
AllPrep DNA/RNA Kits |
| DNA, RNA, and protein |
Tissue |
AllPrep DNA/RNA/Protein Mini Kit |
| QIAcube |
Purification of DNA, RNA, and protein |
Up to 12 samples per run |
| EZ1 Advanced |
Purification of DNA and RNA from human samples |
Up to 6 samples per run |
| EZ1 Advanced XL |
Purification of DNA and RNA from human samples |
Up to 14 samples per run |
| QIAxcel |
Electophoretic analysis of DNA fragments and RNA |
Up to 96 samples per run |
| QIAgility |
Reaction setup |
Up to 384 samples per run |
| Rotor-Gene Q |
Real-time PCR and high-resolution melting (HRM) analyses |
Up to 100 samples per run |
| PyroMark Q24 |
Methylation and mutation analyses |
Up to 24 samples per run |
操作流程
The TissueLyser LT works in combination with the TissueLyser LT Adapter. Up to twelve 2 ml tubes, each containing a sample and a bead, are loaded into the adapter, which is securely fastened to the piston of the TissueLyser LT. The piston moves up and down rapidly, leading to simultaneous disruption and homogenization of the samples due to the beating and grinding action of the beads.
The TissueLyser LT has a small footprint of 15 cm x 27 cm, allowing installation in any laboratory. As each sample is safely sealed within its own tube, the TissueLyser LT is able to disrupt multiple samples without any risk of cross-contamination. If disrupting fresh or frozen samples, the TissueLyser LT Adapter can be precooled on dry ice to prevent degradation of nucleic acids and proteins (see figures "High-quality DNA from plant tissues" and "Intact RNA from plant tissues"). Tissues stored in Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein) or in RNAprotect Tissue Reagent (to stabilize RNA) require no precooling of the adapter.
应用
The ability to process up to 12 samples per run makes the TissueLyser LT the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. The TissueLyser LT enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in the TissueLyser LT Adapter, which holds up to twelve 2 ml sample tubes. Sample purification can be performed manually, or can be automated using the QIAcube, QIAsymphony SP, QIAxtractor, EZ1 Advanced, EZ1 Advanced XL, or BioRobot, or BioSprint instruments.
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特点
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参数
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尺寸
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Weight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
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破碎原理
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High-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
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特点
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Simultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
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与仪器兼容的试剂盒
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All kits for purification of RNA/DNA/protein
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该仪器的实验方案/主要应用
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Sample preparation/sample disruption
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技术
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Bead Mill
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常规运行时间
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40 sec - 5 min, depending on protocol
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FAQ ID -2545
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FAQ ID -2546
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FAQ ID -2547
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FAQ ID -2549
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FAQ ID -2550
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FAQ ID -2551
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FAQ ID -2552
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FAQ ID -2553
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FAQ ID -2554
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FAQ ID -2555
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FAQ ID -374
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FAQ ID -2548
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For disruption of up to 12 samples on the TissueLyser LT
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显示更多
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For disruption of up to 12 biological samples
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显示更多
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显示更多
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图片
高效的组织破碎。
使用TissueLyser LT或TissueLyser II破碎不同的大鼠组织。[A] 在QIAcube全自动核酸纯化仪上使用DNeasy Blood & Tissue Kit从25 mg样本中纯化DNA。利用分光光度计测定DNA产量。[B] 在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit (皮肤、心脏和肺) 或RNeasy Lipid Tissue Mini Kit (大脑),从20 mg样本中纯化RNA。利用分光光度计测定RNA产量。
从各种类型组织中纯化完整的蛋白,用于分析。
将多只大鼠的组织(每份25 mg)稳定于Allprotect Tissue Reagent中,然后使用Tissuelyser LT或TissueLyser II破碎。使用Qproteome Mammalian Protein Prep Kit纯化样本中的总蛋白,之后通过Western印迹进行分析。[A]用Ponceau S染色染印膜,并且[B]进行Western印迹。LT:Tissuelyser LT;II:TissueLyser II ;M:分子量标准。
从动物组织中纯化高品质DNA。
将多种大鼠组织(每份25 mg)稳定于Allprotect Tissue Reagent中,然后使用TissueLyser LT或TissueLyser II进行破碎。在QIAcube全自动核酸纯化仪上使用DNeasy Blood & Tissue Kit纯化DNA,然后使用HotStarTaq Plus Master Mix Kit和PGK1引物体系进行PCR反应。在QIAxcel上使用QIAxcel DNA High Resolution Kit分析120 bp的PCR产物。LT: TissueLyser LT; II: TissueLyser II; M: 分子量标准。
从植物组织中纯化高品质DNA。
使用TissueLyser LT或TissueLyser II在预冷的适配器中破碎多种植物组织(每份100 mg)。在QIAcube全自动核酸纯化仪上使用DNeasy Plant Mini Kit纯化DNA,然后在琼脂糖凝胶上进行电泳分析。LT: TissueLyser LT; II: TissueLyser II; M: 分子量标准。
从植物组织中纯化完整的RNA。
使用TissueLyser LT在预冷的适配器中破碎不同的植物组织 (各100 mg),重复两次。使用RNeasy Plant Mini Kit纯化RNA,然后进行琼脂糖凝胶电泳分析。清晰的核糖体RNA条带表示完整RNA的纯化。
纯化的RNA的RIN值高。
使用TissueLyser LT破碎大鼠的心脏和肺 (各20 mg)。在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit纯化RNA,然后在Agilent 2100 Bioanalyzer上进行分析。RIN值分别为8.3 (心脏) 和9 (肺)。
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