QIAamp DNA Stool Mini Kit
For isolation of DNA from stool
- Rapid isolation of high-quality, ready-to-use DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Complete removal of contaminants and inhibitors
The QIAamp DNA Stool Mini Kit provides silica membrane-based purification of up to 30 μg genomic, bacterial, viral, and parasite DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. The combined action of InhibitEX, a unique adsorption resin, and an optimized buffer leads to removal of PCR inhibitors. The convenient QIAamp spin-column procedure provides purification in only 50 minutes. Purification of DNA using the QIAamp DNA Stool Mini Kit can be automated on the QIAcube.
The QIAamp® DNA Stool Mini Kit (50) (cat. no. 51504) and the InhibitEX Tablets (100) (cat. no. 19590) are no longer manufactured. Instead, we offer you the QIAamp Fast DNA Stool Mini Kit (50) (cat. no. 51604) as an alternative product.
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QIAamp DNA Stool Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。
QIAamp DNA Stool Mini实验流程。|去除PCR抑制剂。|
|使用[A] 传统的硅胶纯化技术和[B] QIAamp DNA Stool Mini Kit从19个粪便样本中纯化DNA。为说明纯化的洗脱液中有无抑制剂存在,将各种洗脱液5 µl加入包含GFP基因的10 pg质粒模板的PCR混合物中。加入各种QIAamp洗脱液均可成功完成GFP基因的扩增,而加入采用包含绿色荧光蛋白的传统技术制备的洗脱液,只有2个扩增反应成功完成。M:分子量标准;C:阳性PCR对照。|
性能
The QIAamp DNA Stool Mini Kit allows rapid purification of DNA from fresh or frozen human stool or other sample types with high concentrations of PCR inhibitors. Up to 220 mg stool can be processed routinely, and larger amounts can be processed with additional Buffer ASL. Typical yields of 10–30 µg are obtained in 50 minutes, and DNA is eluted in 200 µl. The purified DNA is sized up to 50 kb. DNA of this length denatures completely and has the highest amplification efficiency. Highly pure DNA is ready for direct use in downstream amplification reactions (see figure "Removal of PCR inhibitors").
DNA that has been purified using the QIAamp DNA Stool Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease research, and screening.
原理
DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors are removed by the combined action of InhibitEX, a unique adsorption resin, and an optimized buffer. Rigorous lysis using proteinase K ensures high yields of all types of DNA common in stool, including colorectal epithelial cells, bacteria, viruses and other gastrointestinal pathogens.
Highly pure DNA ready for direct use in downstream amplification reactions is purified in about 50 minutes. QIAamp sample preparation technology is fully licensed.
操作流程
The QIAamp DNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart " Procedure"). Optimized buffers and enzymes lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. PCR inhibitors are removed by the combined action of InhibitEX and an optimized buffer. Proteinase K lysis ensures high yields of all types of DNA common in stool. Remaining impurities are efficiently removed in two wash steps. Amplification-ready DNA is then eluted in low-salt buffer.
应用
The QIAamp DNA Stool Mini Kit is for isolation of genomic, bacterial, viral, and parasite DNA from the following sample types:
- Fresh stool
- Frozen stool
- Other sample types with high concentrations of PCR inhibitors
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特点
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参数
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应用
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PCR, blotting
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洗脱体积
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200 µl
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规格
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Spin column
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主要样本类型
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Stool samples
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处理
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Manual (centrifugation or vacuum)
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纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白
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Genomic DNA, bacterial DNA, parasite DNA, viral DNA
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样本量
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220 mg
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技术
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Silica technology
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每次运行或制备时间
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50 minutes
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产量
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10–30 µg
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图片
QIAamp DNA Stool Mini实验流程。
去除PCR抑制剂。
使用[A] 传统的硅胶纯化技术和[B] QIAamp DNA Stool Mini Kit从19个粪便样本中纯化DNA。为说明纯化的洗脱液中有无抑制剂存在,将各种洗脱液5 µl加入包含GFP基因的10 pg质粒模板的PCR混合物中。加入各种QIAamp洗脱液均可成功完成GFP基因的扩增,而加入采用包含绿色荧光蛋白的传统技术制备的洗脱液,只有2个扩增反应成功完成。M:分子量标准;C:阳性PCR对照。
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