Long-range PCR, multiplex PCR, RT-PCR과 같은 민감도가 높은 PCR에도 적합함
dNTP Set, PCR Grade is a complete set of individual, highly pure dNTPs. Each individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP to the desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP in water (pH 7.5), each at a concentration of 10 mM. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.
100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50 µl PCR reactions
dNTP Set and dNTP Mix, PCR Grade are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
The performance of each lot of dNTP Set and Mix, PCR grade, is tested in a specific polymerase chain reaction requiring all four nucleotides.
Highly pure dNTPs are a crucial requirement for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield. Due to their high purity, dNTP Set and dNTP Mix, PCR Grade, are highly suitable for a variety of robust and sensitive PCR techniques.
Protocols for the use of dNTP Set and Mix can be found in the appropriate QIAGEN PCR Kit. Selected kit handbooks can be downloaded from our Web site.
dNTP Set and dNTP Mix, PCR Grade are suitable for use in all standard PCR techniques and all sensitive PCR techniques. In addition, these products are ideal for use in combination with all common PCR and RT-PCR enzymes, including Taq DNA Polymerase, HotStarTaq DNA Polymerase, and our reverse transcriptase enzymes.
dNTP Set and dNTP Mix, PCR Grade can be used for applications such as:
Standard, single-cell, long-range, multiplex, and high-fidelity PCR
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)