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RNAprotect Cell Reagent

Cultured cell 또는 보관된 cell을 곧바로 안정화 시켜 샘플을 편리하게 이동할 수 있습니다
  • 즉각적인 RNA stabilization으로 배지 제거 없이 cultured cells 보관 가능
  • Cell의 보관 및 운반 조건 — 하루: 30도씨, 7일: 15–25도씨, 4주: 2– 8도씨
  • -20ºC 또는 -80ºC에서 세포 보관

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

Cat No./ID: 76526
RNAprotect Cell Reagent (250 ml)
₩447,000
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250 ml RNAprotect Cell Reagent
The RNAprotect Cell Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Effective inhibition of PMA induction of c-fos expression.

Incubation of PMA-stimulated Jurkat cells in RNAprotect Cell Reagent prevents PMA induction of c-fos expression as well as degradation of c-fos transcript, demonstrating stabilization of RNA by RNAprotect Cell reagent (each bar represents the mean of duplicate cell samples).

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More reliable quantification of CD83 transcript.

Storage of cytokine-stimulated immature dendritic cells overnight at –20ºC in RNAprotect Cell Reagent instead of PBS allows quantification of higher levels of CD83 mRNA, indicating stabilization of RNA by RNAprotect Cell Reagent. (Data kindly provided by Dr Alexander Prechtel and Prof. Alexander Steinkasserer, University Hospital Erlangen, Erlangen, Germany.)

Performance
RNAprotect Cell Reagent prevents induction of gene expression in treated cells and preserves and stabilizes transcript levels during storage of cell samples (see figures " Effective inhibition of PMA induction of c-fos expression" and " More reliable quantification of CD83 transcript").
Principle

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

Procedure
RNAprotect Cell Reagent immediately stabilizes the gene expression profile in cells. With other technologies, it is necessary to wash or process the cells (e.g., trypsin treatment) prior to adding an RNA stabilization reagent. Changes in expression that may occur during the washing or processing steps are eliminated by adding RNAprotect Cell Reagent directly to cells in culture medium or buffer. Instantaneous RNA stabilization with RNAprotect Cell Reagent can also be extended to pelleted cells not in medium or storage solution. The amount of starting material must not exceed 1 x 107 cells.
Applications

Cells in RNAprotect Cell Reagent can be conveniently stored and transported at ambient temperature before further processing and RNA purification. The RNA can be purified using the RNeasy Plus Mini Kit, or purified simultaneously with DNA from the same cell sample using an AllPrep Kit.

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