In vitro diagnostic를 목적으로 사용하기 위해 human plasma와 serum에서 viral nucleic acid를 분리정제할 수 있습니다
  • Universal nucleic acid purification system compatible with other IVD products
  • Qualitative와 quantitative NAT assays에서 핵산의 고감도 detection
  • 500 ul 샘플로 부터 20 또는 60 ul의 nucleic acid로 농축 가능함
  • 신속한 분리 정제, cross-contamination 위험의 최소화

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA for in vitro diagnostic use.

Product Product no. Cat. no. List price:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
QIAamp DSP Virus Kit
For 50 preps: QIAamp MinElute Columns, buffers, reagents, tubes, column extenders, VacConnectors
60704
Inquire

The QIAamp DSP Virus Kit is intended for in vitro diagnostic use.


  • Main Image Navi
QIAamp DSP Virus Kit procedure.|Universal nucleic acid purification system that easily fits into diagnostic workflows.|
||
Performance

Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification or other modification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure
The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute.  Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, "Procedure").
Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.

Feature
Specifications
Applications PCR, RT-PCR, LCR
CE/FDA/IVD compatible CE/IVD
Elution volume 20 µl, 60 µl
Format Sample tubes
Main sample type Serum, plasma
Processing Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 500 µl
Technology Silica technology
Time per run or per prep 25 minutes
Yield Varies

You are not authorized to download the resource

References
0