Large-Scale Plasmid Purification — Product Overview

QIAGEN offers a wide range of products for large-scale plasmid preps
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For large-scale purification of molecular biology or transfection grade plasmid DNA, trust QIAGEN's wide range of products! Speed, superior quality, high purity, and unmatched yields are just some of the benefits that can be experienced with QIAGEN's large-scale plasmid purification products. Efficient and reliable endotoxin removal ensures success in transfection experiments. Streamlined protocols and additonal features such as reagents for visualization of optimum lysis conditions, assure maximal convenience.

No matter what your downstream application, we've got a product that best matches your needs!

 

 

View our helpful selection guide below to choose the right product for your downstream application:


 
Large-scale plasmid purification kits from QIAGEN
Application HiSpeed Plasmid Kits QIAGEN Plasmid/
QIAfilter Plasmid Kits
QIAGEN Large- Construct Kit CompactPrep Plasmid Kits QIAGEN Plasmid Plus Kits Endofree 
Plasmid Kits 
Ultrapure Plasmid DNA Prep. Services
  Large-scale plasmid kits
in spin-column format
    Preclinical-scale
(1–100 mg)
Transfection of primary/sensitive cells         X X X
Gene silencing X X X X X X X 
Microinjection X X X   X X X  X
Research on gene therapy           X X X 
Transfection of most cells* X X X   X  X X X
Demanding enzymatic modifications X X X X X X X X
Library constructions (BACs and cosmids) X X X X X X X
Transfection of robust cells* X X   X  X X X X
In vitro transcription X X   X X X X X
In vitro translation X X    X X  X X X
High-quality sequencing X X X  X  X  X X X
Cloning X  X  X  X X  X X X
Probe generation X  X X  X X X X X
High-throughput sequencing X X X X X X X X
PCR X X  X  X X  X X X
Restriction digestion X  X  X X X X X X
Transformation X X  X X  X  X X X
X: Recommended kit; X: DNA grade or kit format can be used but may not be optimal.
This table indicates minimum DNA purity requirements. Higher DNA purity may improve performance or be required for some applications.
* Transfection of sensitive cells may require a higher DNA grade.
Long reads and difficult or heterogeneous templates may require a higher DNA purity.

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7
Ultrapure 100 Column용 buffer set
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 Standard application을 위한 molecular biology grade의 plasmid 혹은 cosmid DNA를 750 ug까지 초고속으로 분리 정제할 수 있습니다
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Standard application을 위한 molecular biology grade의 plasmid 혹은 cosmid DNA를 200 ug까지 초고속으로 분리 정제할 수 있습니다
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QIAGEN Large-Construct Kit
 150 ug BAC, PAC, and P1 DNA를 150 ug까지 혹은 cosmid DNA, free of genomic DNA 400 ug까지 분리 정제할 수 있습니다
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다양한 종류의 샘플에서 고분자의 DNA를 100 ug까지 분리할 수 있습니다
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다양한 종류의 샘플에서 고분자량의 DNA를 500 ug까지 분리할 수 있습니다
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다양한 종류의 샘플에서 고분자량의 DNA를 20 ug까지 분리할 수 있습니다
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