QIAcube

For fully automated sample prep using QIAGEN spin-column kits

Features

  • Automation of trusted QIAGEN spin-column kits
  • Elimination of manual processing steps
  • Purification of DNA, RNA, or proteins
  • More free time with affordable automated processing
  • Standardized results and increased productivity

Product Details

The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into the laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run.

Explore the virtual demo to learn more about QIAcube. 

QIAcube operates in conjunction with QIAcube Accessories.

Performance

Highly pure plasmid DNA

The QIAcube together with the QIAprep Spin Miniprep Kit enables fully automated purification of up to 20 µg molecular biology grade plasmid DNA. The fully automated purification procedure — including lysis — provides yields of plasmid DNA comparable to the manual procedure (see figure “ DNA yields comparable to the manual procedure”). Purified DNA is suitable for use in routine molecular biology applications, such as fluorescent sequencing, cloning, or transfection of robust cells (see figure “ Reliable sequencing results”).

High-quality genomic DNA

Using the QIAamp DNA Blood Kit, the QIAcube enables purification of reproducible yields of highly pure genomic DNA (see figure “ Reproducible DNA yields”). DNA purified using the QIAcube performs well in sensitive PCR even when large amounts of eluate are used, demonstrating the high purity of the DNA (see figure “ High PCR performance”).

Efficient purification of viral nucleic acids

The QIAcube together with the QIAamp MinElute Virus Spin Kit enables efficient purification of viral nucleic acids. To evaluate the risk of sample-to-sample carryover during and between runs, the QIAamp MinElute Virus Spin procedure was subjected to rigorous testing using an alternating checkerboard setup of negative and highly positive plasma samples (1 x 108 IU/ml of a typical DNA virus). All of the highly positive samples were detected. All negative samples, in the checkerboard runs and the all-negative runs were unresponsive (see table “No sample carryover detected”).

No sample carryover detected
1 2 3 4 5 6 7 8 9 10 11 12
Run 1 19.5 X 19.4 X 18.8 X X 19.0 X 19.1 X 19.8
Run 2 X 19.1 X 18.9 X 18.8 18.7 X 19.2 X 18.9 X
Run 3 X X X X X X X X X X X
Negative and highly positive (1 x 108 IU/ml typical DNA virus) plasma samples arranged in an alternating sequence on the QIAcube were purified using the QIAamp MinElute Virus Spin Kit and analyzed by real-time RT-PCR. Mean CT value for positive samples was 19. Samples with CT > 45 were regarded as negative. Under these conditions sample carryover was not detected.
Pure, high performance RNA

Together with the QIAcube, the RNeasy Mini Kit enables efficient automated purification of total RNA from animal cells, demonstrated by consistent RNA yields (see figure “ Consistent yields of RNA”). The quality of RNA purified using the QIAcube is comparable to the manual procedure (see figure “ Comparison of automated and manual RNA purification”).

High-quality proteins

The QIAcube and Ni-NTA Spin Kit provide reliable purification of highly pure 6xHis-tagged proteins (see figure “ Highly reproducible protein purification”). Purified proteins are suitable for many applications, including functional investigations, crystallization pre-studies for determination of three-dimensional structure, assays involving protein–protein and protein–DNA interactions, and immunization to produce antibodies.

Efficient cleanup of DNA fragments

The QIAcube makes DNA cleanup easier than ever. Proven QIAquick technology together with optimized protocols enables direct purification of double- or single-stranded DNA fragments, from 100 bp to 10 kb. Primers, nucleotides, enzymes, and other impurities are efficiently removed and recovery of DNA is comparable to the manual procedure (see figure “ High recovery of PCR products”).

See figures

Principle

The QIAcube enables continued use of well-established QIAGEN spin-column kits and eliminates the need for tedious manual steps. The innovative QIAcube controls integrated components including a centrifuge, heated shaker, pipetting system, and robotic gripper. This enables the QIAcube to fully automate more than 40 QIAGEN spin-column kits.

The QIAcube is preinstalled with a variety of protocols for purification of RNA, genomic DNA, plasmid DNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. All standard protocols in the expanding range can also be downloaded free of charge. In addition, customized protocols tailored to meet your specific application demands can also be requested.

Procedure

The QIAcube enables purification of highly pure nucleic acids or proteins using a simple lyse, bind, wash, elute procedure ( see flowchart). Over 40 QIAGEN spin-column kits can be automated on the QIAcube.

Unparalleled ease of use

The QIAcube takes ease of use to a new level. No external PC is required, saving valuable laboratory space. An integrated touch screen simplifies protocol selection, and clear on-screen messages guide the user through worktable setup. For increased ease of use and high process safety, labware and accessories fit onto the worktable only in the correct orientation. In addition, a fully automated load check helps to ensure that samples, reagents, and labware are correctly loaded.

New dedicated kits for DNA and RNA preps

Dedicated QIAcube Kits further simplify automated spin preps on the QIAcube and increase convenience. Kits are currently available for purification of RNA, genomic DNA, and viral RNA. Rotor-adapters supplied with the kits are preloaded with spin columns and elution tubes, delivering greater convenience and time savings. Furthermore, ease of use is increased and user errors minimized. Waste is reduced as the content of the dedicated kits is tailored to purification on the QIAcube and tubes required for the manual procedure are not included.

See figures

Applications

The QIAcube is highly suitable for academic research laboratories as well as pharmaceutical, biotechnology, and biomedical research laboratories performing applications, such as:

  • Sequencing/sequencing analysis
  • Gene expression analysis
  • Genotyping
  • Proteomics

Supporting data and figures

Specifications

FeaturesSpecifications
Centrifuge12,000 x g maximum, swing-out rotor with 12 positions, maximum 45°
HumidityRelative humidity of 10-75% (noncondensing)
Protocols/main application on this instrumentDNA purification, RNA purification, protein purification
Overvoltage categoryII
Power100-120 V AC, 50-60 Hz, 650 VA (North America and Japan), 220-240 V AC, 50-60 Hz, 650 VA (Europe), mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages
Place of operationFor indoor use only
Storage temperature15°C to 30°C in manufacturer's package
Kits designed for this instrumentMost QIAGEN manual kits (e.g., QIAamp, QIAquick, QIAprep)
ShakerSpeed 100-2000 rpm, amplitude 2 mm, heating range of room temperature to 70°C, ramp-up time of <5 min from room temperature to 55°C (+/-)3°C)
TechnologyDepends on kit used
Instrument dimensions650 x 620 x 570 mm
Operating temperature18-28°C
Samples per run (throughput)1–12 samples per run
Pipetting systemSyringe size 1 ml, pipetting range 5-900 µl
ProcessingAutomated
Pollution level2
Technical data of the instrument70.5 kg
SoftwareQIAGEN protocols are preinstalled on the QIAcube or can be downloaded at www.qiagen.com/MyQIAcube
TouchscreenTransmissive TFT, 64 x 86 x 65 mm, white LED backlight, high brightness
Transportation temperature-25°C to 50°C in manufacturer's package
AltitudeUp to 2000 m (6500 ft)

Resources

Safety Data Sheets (1)

FAQ

What applications are offered on the QIAcube?

For an up-to-date list of available protocols, visit QIAcube Standard Protocols.

 

Click here for a virtual demonstration on how the QIAcube operates.

FAQ ID -1403
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?

We recommend to verify the tightness from the system during the periodic maintenance described in the User Manual, and to replace the o-ring as needed.

Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?

Part numbers and prices for QIAcube accessories can be found by clicking here.

FAQ ID -3054
Can the QIAcube heater/shaker be used independently from protocol runs?

Yes. The heated shaker on the QIAcube Connect can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube Connect User Manual, chapter 5 "Operating Procedures", section 5.9 "Independent heater/shaker operation".

The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.

FAQ ID -1422
Are run reports and/or log files available on the QIAcube?

Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

Report files can be automatically transferred from your QIAcube Connect using the QIAsphere Export Tool. For this, just connect your QIAcube Connect to QIAsphere. More information can be found on the QIAsphere webpage.

FAQ ID -1412
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?

Only the recommended Sample Tubes in the specific Protocol Sheet should be used; to interchange the Sample Tubes, a modified protocol for the QIAcube should be requested through the online QIAcube protocol request page.

Can the QIAcube centrifuge be used independently from protocol runs?

You can stop a QIAcube protocol if there is an emergency by pressing Stop Run. To confirm that you want to stop the protocol run, press Yes. To cancel the "Stop Run" command, press No.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed on the touchscreen. For more information, refer to the QIAcube Connect User Manual.

FAQ ID -1423
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?

Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.

 

FAQ ID -1415
How can I decontaminate my QIAsymphony SP / AS system?

The QIAsymphony system has an integrated UV lamp. UV decontamination should be performed daily. It helps to reduce possible pathogen contamination of the QIAsymphony SP/AS worktables. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type. QIAGEN cannot guarantee complete eradication of specific pathogens.

FAQ ID - 3714
Are there any specific cleaning solutions recommended for the QIAcube?

The following disinfectants and detergents are recommended for cleaning the QIAcube:

  • Mikrozid® Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g Mikrozid Liquid)
  • RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door
  • RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
  • 0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)
  • Note: Alcohol is not recommended for cleaning of the door.

Europe 

Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).

 

USA

DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1416
3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?
A custom protocol can be requested through the online QIAcube protocol request page:
< https://www.qiagen.com/qiacube/customized/protocoladd.aspx >.

 

What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?

Up to 12 samples can be processed in a single run.

FAQ ID - 3482
How can I get software updates for the QIAcube?

New software or software updates can be accessed via the QIAcube Web Portal at www.qiagen.com/MyQIAcube. To access them directly, go to User Support.

 

 

FAQ ID -1413
Can purification columns from other suppliers be processed on the QIAcube?

No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.

 

See:  http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.

 

FAQ ID -1406
Can I program my own protocols for the QIAcube?

No. Slightly modified QIAGEN protocols or protocols fully customised to your specific requirements can be requested from QIAGEN. Our dedicated team of specialists has already customised over 5000 protocols.

  1. Go to www.qiagen.com/MyQIAcube  and enter the required information. If our application specialists require additional information, they will contact you directly.
  2. You will be notified by email when your customized protocol is ready for download.
  3. Click on the link in the email to access the download page.

FAQ ID -1405
Can the QIAcube be connected to a laboratory information management system?
Report files can be automatically transferred from your QIAcube Connect using the QIAsphere Export Tool. For this, just connect your QIAcube Connect to QIAsphere and install the QIAsphere Export Tool on a computer connected to the network. More information can be found on the QIAsphere webpage.
FAQ ID -1411
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
The RNeasy Micro protocol with a DNase Digestion step has the same workflow as the manual RNeasy Protect Saliva Mini Kit, which is designed for saliva samples.
What dedicated QIAcube Kits are available?
3325 - What are the catalog numbers for the packing materials for the QIAcube?

The packing materials for the QIAcube are available for purchase with the following catalog numbers: 9018473: Transport box, QIAcube;  9018083: Foam insert, arm securing, QIAcube; 9019890: Foam insert, rotor securing, QIAcube.

 

What are the dimensions and weight of the QIAcube?

The QIAcube Connect has the following weight and dimensions: 

  • Weight: 73 kg (160.9 lb.)
  • Width: 65 cm (25.6 in.)
  • Depth: 62 cm (24.4 in.)
  • Height (door closed): 58 cm (22.8 in.)
  • Height (door open): 86 cm (34 in.)
FAQ ID -1407
What sample tubes can I use on my QIAsymphony SP system?

Please navigate to the Product Page of the QIAsymphony Kit you are using; for example, the QIAsymphony RNA Kit, Cat No./ID: 931636.  Refer to the resources tab and download the labware document. The labware document will list the sample tubes that have been tested for your application. Please contact Technical Service if you wish to use sample tubes not listed in the labware document.

FAQ ID - 3713
How can I decontaminate the QIAcube system?

Please refer to Section 6.2 "Disinfection" and Section 6.6 "Periodic maintenance" of the QIAcube Connect User Manual

FAQ ID -1419
Can the QIAcube rotor and/or buckets be removed for cleaning?

Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.

 

FAQ ID -1410
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?

You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.

Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.

FAQ ID -1414
Do I need to buy special kits for the QIAcube?

No. The beauty of the QIAcube Connect is that you can automate your supported manual spin-column workflow flawlessly. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.

However, in addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and disposable tips are required:

  • Filter-Tips, 200 µL (1024), cat no. 990332,
  • Filter-Tips, 1000 µL (1024), cat no. 990352,
  • Filter-Tips, 1000 µL wide-bore (1024), cat no. 990452.

To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet or the instructions on the QIAcube Connect touchscreen. Even easier to use are the dedicated QIAcube kits.

FAQ ID -1402
Can the QIAcube rotor adapters be autoclaved?
No. Autoclaving the rotor adapters will cause the plastic to deform and the rotor adapters will no longer fit properly into the centrifuge buckets.
FAQ ID -1409
3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
A custom protocol can be requested through the online QIAcube protocol request page: <https://www.qiagen.com/qiacube/customized/protocoladd.aspx >.
FAQ ID - 3323

 

Do I need to discard partially used QIAcube tip racks?

No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.

The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.

FAQ ID -1418
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
Yes. Samples have to be incubated at least 2 h at room temperature (18-22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to three days has no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consist of a centrifugation and a pellet resuspenion step (see the PAXgene Blood miRNA Handbook ).
FAQ ID - 3479