For fully automated sample prep using QIAGEN spin-column kits
For fully automated sample prep using QIAGEN spin-column kits
The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into the laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run.Explore the virtual demo to learn more about QIAcube.
The QIAcube together with the QIAprep Spin Miniprep Kit enables fully automated purification of up to 20 µg molecular biology grade plasmid DNA. The fully automated purification procedure — including lysis — provides yields of plasmid DNA comparable to the manual procedure (see figure “ DNA yields comparable to the manual procedure”). Purified DNA is suitable for use in routine molecular biology applications, such as fluorescent sequencing, cloning, or transfection of robust cells (see figure “ Reliable sequencing results”).
Using the QIAamp DNA Blood Kit, the QIAcube enables purification of reproducible yields of highly pure genomic DNA (see figure “ Reproducible DNA yields”). DNA purified using the QIAcube performs well in sensitive PCR even when large amounts of eluate are used, demonstrating the high purity of the DNA (see figure “ High PCR performance”).
The QIAcube together with the QIAamp MinElute Virus Spin Kit enables efficient purification of viral nucleic acids. To evaluate the risk of sample-to-sample carryover during and between runs, the QIAamp MinElute Virus Spin procedure was subjected to rigorous testing using an alternating checkerboard setup of negative and highly positive plasma samples (1 x 108 IU/ml of a typical DNA virus). All of the highly positive samples were detected. All negative samples, in the checkerboard runs and the all-negative runs were unresponsive (see table “No sample carryover detected”).
Together with the QIAcube, the RNeasy Mini Kit enables efficient automated purification of total RNA from animal cells, demonstrated by consistent RNA yields (see figure “ Consistent yields of RNA”). The quality of RNA purified using the QIAcube is comparable to the manual procedure (see figure “ Comparison of automated and manual RNA purification”).
The QIAcube and Ni-NTA Spin Kit provide reliable purification of highly pure 6xHis-tagged proteins (see figure “ Highly reproducible protein purification”). Purified proteins are suitable for many applications, including functional investigations, crystallization pre-studies for determination of three-dimensional structure, assays involving protein–protein and protein–DNA interactions, and immunization to produce antibodies.
The QIAcube makes DNA cleanup easier than ever. Proven QIAquick technology together with optimized protocols enables direct purification of double- or single-stranded DNA fragments, from 100 bp to 10 kb. Primers, nucleotides, enzymes, and other impurities are efficiently removed and recovery of DNA is comparable to the manual procedure (see figure “ High recovery of PCR products”).
The QIAcube enables purification of highly pure nucleic acids or proteins using a simple lyse, bind, wash, elute procedure ( see flowchart). Over 40 QIAGEN spin-column kits can be automated on the QIAcube.
The QIAcube takes ease of use to a new level. No external PC is required, saving valuable laboratory space. An integrated touch screen simplifies protocol selection, and clear on-screen messages guide the user through worktable setup. For increased ease of use and high process safety, labware and accessories fit onto the worktable only in the correct orientation. In addition, a fully automated load check helps to ensure that samples, reagents, and labware are correctly loaded.
Dedicated QIAcube Kits further simplify automated spin preps on the QIAcube and increase convenience. Kits are currently available for purification of RNA, genomic DNA, and viral RNA. Rotor-adapters supplied with the kits are preloaded with spin columns and elution tubes, delivering greater convenience and time savings. Furthermore, ease of use is increased and user errors minimized. Waste is reduced as the content of the dedicated kits is tailored to purification on the QIAcube and tubes required for the manual procedure are not included.
The QIAcube is highly suitable for academic research laboratories as well as pharmaceutical, biotechnology, and biomedical research laboratories performing applications, such as:
|Centrifuge||12,000 x g maximum, swing-out rotor with 12 positions, maximum 45°|
|Humidity||Relative humidity of 10-75% (noncondensing)|
|Protocols/main application on this instrument||DNA purification, RNA purification, protein purification|
|Power||100-120 V AC, 50-60 Hz, 650 VA (North America and Japan), 220-240 V AC, 50-60 Hz, 650 VA (Europe), mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages|
|Place of operation||For indoor use only|
|Storage temperature||15°C to 30°C in manufacturer's package|
|Kits designed for this instrument||Most QIAGEN manual kits (e.g., QIAamp, QIAquick, QIAprep)|
|Shaker||Speed 100-2000 rpm, amplitude 2 mm, heating range of room temperature to 70°C, ramp-up time of <5 min from room temperature to 55°C (+/-)3°C)|
|Technology||Depends on kit used|
|Instrument dimensions||650 x 620 x 570 mm|
|Samples per run (throughput)||1–12 samples per run|
|Pipetting system||Syringe size 1 ml, pipetting range 5-900 µl|
|Technical data of the instrument||70.5 kg|
|Software||QIAGEN protocols are preinstalled on the QIAcube or can be downloaded at www.qiagen.com/MyQIAcube|
|Touchscreen||Transmissive TFT, 64 x 86 x 65 mm, white LED backlight, high brightness|
|Transportation temperature||-25°C to 50°C in manufacturer's package|
|Altitude||Up to 2000 m (6500 ft)|
We recommend to verify the tightness from the system during the periodic maintenance described in the User Manual, and to replace the o-ring as needed.
Part numbers and prices for QIAcube accessories can be found by clicking here.
Yes. The heated shaker on the QIAcube Connect can be operated independently if the QIAcube is not performing a protocol run. For instructions on how to operate the shaker, refer to the QIAcube Connect User Manual, chapter 5 "Operating Procedures", section 5.9 "Independent heater/shaker operation".
The shaker can be used to heat and shake samples simultaneously, shake samples without heating, or heat samples without shaking.
Yes. At the end of each protocol run on the QIAcube, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.
Report files can be automatically transferred from your QIAcube Connect using the QIAsphere Export Tool. For this, just connect your QIAcube Connect to QIAsphere. More information can be found on the QIAsphere webpage.
Only the recommended Sample Tubes in the specific Protocol Sheet should be used; to interchange the Sample Tubes, a modified protocol for the QIAcube should be requested through the online QIAcube protocol request page.
You can stop a QIAcube protocol if there is an emergency by pressing Stop Run. To confirm that you want to stop the protocol run, press Yes. To cancel the "Stop Run" command, press No.
Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed on the touchscreen. For more information, refer to the QIAcube Connect User Manual.
Yes, this is absolutely necessary. If the lids are not securely fixed in the rotor adapter slots during processing on the QIAcube, they will become detached from the elution tubes and columns during centrifugation and will collect at the bottom of the centrifuge chamber.
The QIAsymphony system has an integrated UV lamp. UV decontamination should be performed daily. It helps to reduce possible pathogen contamination of the QIAsymphony SP/AS worktables. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type. QIAGEN cannot guarantee complete eradication of specific pathogens.
The following disinfectants and detergents are recommended for cleaning the QIAcube:
Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15–30% nonionic surfactants).
DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride).
Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.
Up to 12 samples can be processed in a single run.
No. The QIAcube is designed for fully automated processing of up to 12 samples using QIAGEN spin-column kits. Do not use spin columns manufactured by other suppliers with the QIAcube. Damage caused by use of other types of spin columns will void the instrument warranty.
See: http://www.qiagen.com/products/catalogpagecontrols/header/qiacube/360/default.aspx for a demonstration on how the QIAcube is used with the RNeasy Mini Kit for automated purification of RNA.
No. Slightly modified QIAGEN protocols or protocols fully customised to your specific requirements can be requested from QIAGEN. Our dedicated team of specialists has already customised over 5000 protocols.
Dedicated QIAcube kits are the RNeasy Mini QIAcube Kit, QIAamp DNA Mini QIAcube Kit, the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit. In addition to these kits are the QIAamp PowerFecal Pro DNA QIAcube Kit, the DNeasy PowerSoil Pro QIAcube Kit using Power technology, and the DNeasy Blood & Tissue QIAcube Kit.
The packing materials for the QIAcube are available for purchase with the following catalog numbers: 9018473: Transport box, QIAcube; 9018083: Foam insert, arm securing, QIAcube; 9019890: Foam insert, rotor securing, QIAcube.
The QIAcube Connect has the following weight and dimensions:
Please navigate to the Product Page of the QIAsymphony Kit you are using; for example, the QIAsymphony RNA Kit, Cat No./ID: 931636. Refer to the resources tab and download the labware document. The labware document will list the sample tubes that have been tested for your application. Please contact Technical Service if you wish to use sample tubes not listed in the labware document.
Please refer to Section 6.2 "Disinfection" and Section 6.6 "Periodic maintenance" of the QIAcube Connect User Manual.
Yes, rotor and/or buckets can be removed from the QIAcube for cleaning. For information on how to remove these parts, refer to the QIAcube User Manual.
You can stop a QIAcube protocol if there is an emergency by pressing “Cancel”. To confirm that you want to stop the protocol run, press “OK”. To cancel the 'stop protocol' command, press “Cancel”.
Note: If a protocol run is stopped, the run cannot be restarted. The samples must be processed manually. The last protocol step performed by the QIAcube will be displayed in the touch screen. For more information, refer to the QIAcube User Manual.
No. The beauty of the QIAcube Connect is that you can automate your supported manual spin-column workflow flawlessly. The manual kits contain sufficient buffers and reagents to process the number of spin columns provided with the kit.
However, in addition to the purification kit, Rotor Adapters (10 x 24), cat. no 990394, and disposable tips are required:
To find out which filter-tips are required for a specific application, refer to the corresponding protocol sheet or the instructions on the QIAcube Connect touchscreen. Even easier to use are the dedicated QIAcube kits.
No. The QIAcube is equipped with an optical sensor, which determines the tip size and the number of tips loaded onto the worktable prior to starting a run.
The QIAcube can use partially used tip racks from previous runs, provided that the racks contain sufficient tips to process the number of samples loaded for the current run.