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Penta·His Biotin Conjugate

HHHHH epitope을 가진 6xHis-tagged proteins을 높은 민감도로 detection 할 수 있습니다
  • 6xHis-tagged proteins에 대한 고감도 검출
  • Flexible detection using any steptavidin or avidin conjugate

In detection procedures, the Penta·His Conjugate binds to a protein's His tag and the complex is localized using avidin derivatives, such as streptavidin, which can be conjugated to fluorescent dyes (e.g., streptavidin-R-PE) or to other proteins (e.g., HRP, AP) used in detection procedures.

Cat No./ID: 34440
Penta·His Biotin Conjugate
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125 μl Penta·His Biotin Conjugate, 200 μg/ml
Performance of Penta·His Biotin Conjugate
Detection: Five consecutive histidine residues
Antibody isotype: Mouse IgG1
Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
Form: Stabilized stock solution
Working solution: Dependent on application

The Penta·His Biotin Conjugate can be used in conjunction with any streptavidin or avidin conjugate, delivering high flexibility in assay development.

The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.

Penta·His Biotin Conjugates allow colorimetric, chromogenic, chemiluminescent, or fluorescent detection of His-tagged proteins in western and dot blot, ELISA and other immunoassay procedures, and fluorescence-activated cell sorting (FACS).

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Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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