RNase A

QIAGEN Ribonuclease A (RNase A) is endonuclease-free and quality-controlled for use in plasmid purification procedures for digestion of RNA. This ready-to-use solution has the same specifications as the RNase supplied in all QIAGEN plasmid DNA purification kits. An aliquot of the solution must be diluted into Buffer P1 to the appropriate concentration.

QIAGEN Ribonuclease A is Endonuclease-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.  

RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.  

RNase A is very active under a wide range of reaction conditions and is difficult to inactivate. 

QIAGEN RNase A does not need to be boiled before use. 

Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.  

• Plasmid and genomic DNA preparation
• Removal of RNA contamination from recombinant protein preparations
• Cleanup of RNA from enzymatic reactions