DNA 분리 정제하는 동안 RNase digestion을 합니다
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
RNase A
카탈로그 번호 / ID. 19101
2.5 ml (100 mg/ml; 7000 units/ml, solution)
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*Offer valid on orders placed from December 1, 2025, to March 31, 2026, or while stocks last. Offer void where prohibited and cannot be combined with any other promotion. Offer is only valid in the following countries: Australia, Austria, Belgium, Canada, Denmark, Finland, France, Germany, Italy, Luxembourg, Netherlands, New Zealand, Norway, Poland, South Africa, Sweden, Switzerland, United Kingdom, United States of America. Freight and delivery costs are not included. It is the customer's responsibility to ensure that acceptance of this offer will not violate any internal policies of the customer's organization and applicable laws and regulations.
RNase A은/는 분자생물학 분야에 사용하기 위한 것입니다. 이 제품은 질병의 진단, 예방, 또는 치료용이 아닙니다.
Get 5% off the enzyme of your choice.
Use the code ENZ2526 at checkout. Offer valid from December 1, 2025, to March 31, 2026. T&Cs* apply.
특징
- Selectively cleaves single-stranded RNA
- Active under a wide range of reaction conditions
- DNase-free and quality-controlled
- Does not need to be boiled before use
제품 세부 정보
QIAGEN Ribonuclease A (RNase A) is endonuclease-free and quality-controlled for use in plasmid purification procedures for digestion of RNA. This ready-to-use solution has the same specifications as the RNase supplied in all QIAGEN plasmid DNA purification kits. An aliquot of the solution must be diluted into Buffer P1 to the appropriate concentration.
성능
QIAGEN Ribonuclease A is DNase-free and quality-controlled for use in plasmid purification procedures for the digestion of RNA.
RNase A is widely used to remove contaminating RNA during the isolation of plasmid and genomic DNA. RNase A is also used to remove contaminating RNA from DNA samples after extraction or PCR, especially if downstream applications (e.g., cloning or sequencing) require RNA-free DNA.
RNase A is very active under a wide range of reaction conditions and is difficult to inactivate.
QIAGEN RNase A does not need to be boiled before use.
| Feature | RNase A |
|---|---|
| Quantity | 2.5 mL |
| Concentration | 100 mg/; 7000 units/ml, solution |
| Unit definition | One Kunitz unit is the amount of enzyme causing the hydrolysis of RNA at a rate such that the velocity constant (k) equals unity at 25°C and pH 5.0 |
절차
Before use in plasmid and DNA purification, centrifuge QIAGEN RNase A briefly, and then add into Buffer P1 to obtain a final concentration of 100 μg/mL. Store Buffer P1 at 2–8°C after addition of RNase A. For detailed instructions on RNase A usage during plasmid and DNA purification, please refer to the corresponding kit handbook.
응용 분야
- Plasmid and genomic DNA preparation
- Removal of RNA contamination from recombinant protein preparations
- Cleanup of RNA from enzymatic reactions
리소스
안전보건자료 (1)
브로셔 및 가이드 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
3314-What is the concentration of RNase A sold separately?