RNeasy PowerBiofilm KitFor the isolation of high quality total RNA from biofilm samples
Overcome inefficient cell lysis with the RNeasy PowerBiofilm Kit. Mechanical and chemical lysis are combined with Inhibitor Removal Technology to isolate pure RNA free from inhibitors typical of biofilms, including humic acid, metals, salts and pesticides. The resulting RNA is then ready for further analysis with downstream applications.
The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
The RNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Figure 1. High-quality RNA yield from a range of biofilms.
High-quality and yield RNA was recovered from samples regardless of biofilm origin. Several types of biofilms were collected and RNA isolated from those samples. Resulting RNA quality was assessed through gel electrophoresis; RNA yield was determined using NanoDrop.
*All yields represent combined results from multiple experiments and collaborators.
Performance
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Sample to Insight
Location:
Kenya
Language:
English
© QIAGEN 2013–2019. All rights reserved.
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RNeasy PowerBiofilm Kit
For the isolation of high quality total RNA from biofilm samples
- High-quality RNA isolation from all biofilms, challenging dental plaques and microbial mats
- High RNA yields with pre-treatment, mechanical and chemical lysis
- Efficient elimination of PCR inhibitors with Inhibitor Removal Technology
Overcome inefficient cell lysis with the RNeasy PowerBiofilm Kit. Mechanical and chemical lysis are combined with Inhibitor Removal Technology to isolate pure RNA free from inhibitors typical of biofilms, including humic acid, metals, salts and pesticides. The resulting RNA is then ready for further analysis with downstream applications.
The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
Cat No./ID: 25000-50
RNeasy PowerBiofilm Kit (50)
For the isolation of high quality total RNA from biofilm samples
|
The RNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance
Features |
Specifications |
Bead size | 0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix |
Binding capacity | Up to 40 µg per prep |
Format | Silica Spin Filter Tubes |
Processing | Bead beating |
Sample size | 0.05 - 0.20 g |
Sample types | All biofilms from cultured single cell layers to microbial mats |
Storage temperature | Store lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C) |
Throughput | 1-24 samples |
Time per run or per prep | 42 minutes (+15 minute DNase step) |