For the isolation of high quality total RNA from biofilm samples
High-quality RNA isolation from all biofilms, challenging dental plaques and microbial mats
High RNA yields with pre-treatment, mechanical and chemical lysis
Efficient elimination of PCR inhibitors with Inhibitor Removal Technology
Overcome inefficient cell lysis with the RNeasy PowerBiofilm Kit. Mechanical and chemical lysis are combined with Inhibitor Removal Technology to isolate pure RNA free from inhibitors typical of biofilms, including humic acid, metals, salts and pesticides. The resulting RNA is then ready for further analysis with downstream applications.
The RNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm RNA Isolation Kit (50).
For the isolation of high quality total RNA from biofilm samples
The RNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Figure 1. High-quality RNA yield from a range of biofilms.
High-quality and yield RNA was recovered from samples regardless of biofilm origin. Several types of biofilms were collected and RNA isolated from those samples. Resulting RNA quality was assessed through gel electrophoresis; RNA yield was determined using NanoDrop.
*All yields represent combined results from multiple experiments and collaborators.
Figure 2. Improved success of downstream applications.
Successful reverse transcription of RNA performed using the QuantiTech® Reverse Transcription Kit. Universal 16S rRNA gene (StreF/StreR) primers were used. This generated a 1,243 bp product. PCR was run using the KAPATM Fast HotStart Kit.
Figure 3. More positive results.
The E. coli 6-point standard curve is represented by grey curves. Reverse transcribed RNA isolated from lagoon rock biofilms are represented by blue curves.
Performance
Features
Specifications
Bead size
0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix
Binding capacity
Up to 40 µg per prep
Format
Silica Spin Filter Tubes
Processing
Bead beating
Sample size
0.05 - 0.20 g
Sample types
All biofilms from cultured single cell layers to microbial mats
Storage temperature
Store lyophilized DNase I at 4°C
All other kit reagents and components should be stored at room temperature (15-30°C)
