Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?

The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated using the QIAcube Connect. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.

QIAzol Lysis Reagent is optimized for lysis of fatty tissues. The standard QIAzol protocol provides an easy-to-follow procedure, combining QIAzol lysis with standard homogenization methods and isopropanol precipitation.

miRNeasy Kits enable purification of total RNA from all types of animal tissues and cells, including difficult-to-lyse tissues and small tissue and cell samples. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required when using the miRNeasy Micro or Mini Kit and an additional RNeasy 96 plate when using the miRNeasy 96 Kit). Purification of 1–12 samples can be automated on the QIAcube Connect. High-throughput processing in convenient 96-well format can be performed using centrifugation or vacuum procedures. The NEW miRNeasy Tissue/Cells Advanced Mini Kit – get excellent results faster without phenol/chloroform using our QIAzol free Advanced chemistry. Try it today.

The miRNeasy Serum/Plasma Kit is designed for purification of cell-free total RNA — primarily miRNA and other small RNA — from small volumes of serum and plasma. RNA from serum and plasma typically consists of molecules <100 nucleotides. Purification can be automated on the QIAcube Connect. The NEW miRNeasy Serum/Plasma Advanced Kit – get excellent results faster without phenol/chloroform using our QIAzol-free Advanced chemistry. Try it today.