Puregene Accessories

For purification of archive-quality DNA from a wide range of sample types

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Proteinase K (5 ml)

Cat. No. / ID:  158146

5 ml Puregene Proteinase K
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EnzymeSolution
Enzyme
Solution
Type
Proteinase K
RNase A
Lytic
Volume
5 ml
650 µl
The implementation of an in-house manufacturing solution has lead to product name and catalogue number changes in the Puregene portfolio . This change will not reflect on the quality and performance of the products.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Archive-quality DNA for long-term storage
  • Reproducible DNA purification
  • Convenient, scalable purification procedure

Product Details

Puregene Accessories used in combination with Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.

To improve availability address demand more efficiently we implemented an in-house manufacturing solution. This leads to product name and catalogue number changes . This Change will not reflect on the quality and performance of the products. The legacy products will be discontinued on 28.02.2022 and be available only until stocks last.

Please use the table below in the Performance section to find the product you need.

Performance

 

Catalog number changes in the Puregene line.
NEW product name NEW cat.no. Old product name Old cat. no.
158103 Puregene RBC Lysis Solution (450 ml) 158902 RBC Lysis Solution(450 ml)
158106 Puregene RBC Lysis Solution (1000 ml) 158904 RBC Lysis Solution (1000 ml)
158113 Puregene Cell Lysis Solution (125 ml) 158906 Cell Lysis Solution (125 ml)
158116 Puregene Cell Lysis Solution (1000 ml) 158908 Cell Lysis Solution (1000 ml)
158123 Puregene Protein Precipitation Sol. (50 ml) 158910 Protein Precipitation Solution (50 ml)
158126 Puregene Protein Precipitation Solution (350 ml) 158912 Protein Precipitation Solution (350 ml)
158133 Puregene DNA Hydration Solution (100 ml) 158914 DNA Hydration Solution (100 ml)
158136 Puregene DNA Hydration Solution (500 ml) 158916 DNA Hydration Solution (500 ml)
158143 Proteinase K (650 µl) 158918 Puregene Proteinase K (650 µl)
158146 Proteinase K (5 ml) 158920 Puregene Proteinase K (5 ml)
158153 RNase A Solution (650 µl) 158922 RNase A Solution (650 µl)
158156 RNase A Solution (5 ml) 158924 RNase A Solution (5 ml)
158183 Glycogen Solution (500 µl) 158930 Glycogen Solution (500 µl)
158186 Glycogen Solution (5 ml) 949002 Autopure Glycogen Solution (5 ml)

Principle

Using Puregene Kits, cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.

Procedure

Archive-quality DNA 

Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb. 

Applications

DNA purified using Puregene Accessories and Puregene Kits is highly stable and suited for use in a wide range of applications, such as:

  • DNA archiving
  • PCR
  • SNP analysis
  • Southern blotting
  • NGS
  • Long-read sequencing

Resources

Safety Data Sheets (1)
Kit Handbooks (1)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, body fluids, cultured cells, and tissue
Certificates of Analysis (1)

Publications

Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.
Hoyo C; Murtha AP; Schildkraut JM; Jirtle RL; Demark-Wahnefried W; Forman MR; Iversen ES; Kurtzberg J; Overcash F; Huang Z; Murphy SK;
Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975

FAQ

What is the composition of RBC Lysis Solution?
The exact composition of RBC Lysis Solution is confidential. RBC Lysis Solution is a component of Gentra Puregene Blood Kits. RBC Lysis Solution selectively lyses human red blood cells leaving white blood cells intact. You can order RBC Lysis Solutin separately.
FAQ ID -2808
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?

The composition of DNA Hydration Solution is as follows:

  • 10 mM Tris
  • 1 mM EDTA
  • pH 7–8

DNA Hydration Solution is included in QIAGEN’s Gentra Puregene Kits for DNA purification and can also be bought separately from QIAGEN.

FAQ ID -2813
What is the composition of Cell Suspension Solution?
The detailed composition of Cell Suspension Solution is confidential. Cell Suspension Solution is a component of the Gentra Puregene Yeast/Bacteria Kit. Essentially, it is an isotonic solution that prevents spheroblasts from bursting. Cell Suspension Solution can be ordered separately.
FAQ ID -2811
What is the composition of Protein Precipitation Solution?
The exact composition of Protein Precipitation Solution is confidential. Protein Precipitation Solution is a component of Gentra Puregene Kits for DNA purification. Essentially, this is a high-salt buffer that lowers the solubility of proteins. Protein Precipitation Solution can be ordered separately.
FAQ ID -2810
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
The exact composition of Glycogen Solution is confidential. The Glycogen concentration is 20mg/mL. Glycogen Solution is a component of Gentra Puregene Kits for DNA purification. During isopropanol precipitation, Glycogen Solution acts as a nucleic acid carrier and helps to efficiently precipitate small amounts of DNA. In addition, it facilitates visualization of the DNA pellet. Glycogen Solution can be purchased separately.

 

Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.

 

 

FAQ ID -2812
What is the composition of Cell Lysis Solution?
The exact composition of Cell Lysis Solution is confidential. Cell Lysis Solution is a component of Gentra Puregene Kits for DNA purification. It functions by enabling cell lysis. Cell Lysis Solution can be purchased separately.
FAQ ID -2809
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
The Puregene RNase A and Autopure RNase A solutions both have a concentration of 4 mg/ml.

 

FAQ ID -3083
What do you recommend for the cleanup of genomic DNA (gDNA)?

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

 

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

 

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618