PAXgene Tissue DNA Kit
For purification of DNA from tissues fixed and stabilized with the PAXgene Tissue System
The PAXgene Tissue DNA Kit is intended for the purification of DNA from PAXgene Tissue-fixed (PF) and PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue samples. The kit is intended to be used as part of the PAXgene Tissue System, which also includes the PAXgene Tissue FIX Container and PAXgene Tissue STABILIZER Concentrate for the collection, fixation, stabilization and storage of tissues samples.
Specifications for fixation and storage conditions in PAXgene Tissue Fix and PAXgene Tissue Stabilizer were determined using animal tissues.
Total DNA isolated with the PAXgene Tissue DNA Kit is highly pure. DNA has A260/A280 ratios of 1.7–1.9, and absorbance scans show a symmetrical peak at 260 nm confirming the high purity of genomic DNA. Contamination is minimal and purified DNA is ready to be used in downstream applications with no detectable inhibition of PCR (see High-quality DNA from PFPE tissue with preserved morphology and DNA without chemical modification can be used for demanding downstream applications).
The PAXgene Tissue DNA Kit enables purification of DNA from tissues fixed and stabilized using the PAXgene Fixation and Stabilization reagents, which preserve tissue morphology and biomolecule integrity by avoiding destructive crosslinking and degradation found in formalin-fixed tissues. The purified DNA has no inhibitory chemical modifications and thus, can be used in sensitive downstream applications.
PAXgene Tissue-fixed (PF) or PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue samples are lysed in Buffer TD1 with proteinase K. Conditions are adjusted for optimal binding of DNA to the silica membrane and the lysate is loaded on the PAXgene DNA spin column. DNA binds to the membrane while contaminants pass through. Enzyme inhibitors are effectively removed with subsequent washes and pure DNA is eluted in a low-salt elution buffer (see The PAXgene Tissue DNA procedure).
The PAXgene Tissue DNA Kit provides 2 protocols for purification of DNA from different starting materials: from sections of PFPE tissues and from PF tissue samples (without paraffin embedding).
DNA purification using the PAXgene Tissue DNA Kit can be uatomated on the QIAcube, enabling:
Two protocols are available for automated purification from different starting materials:
The purified DNA is ready to use in a wide range of downstream applications, including:
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