QIAamp DNA Blood Maxi Kit
For purification of up to 600 µg genomic, mitochondrial, or viral DNA from blood
The QIAamp DNA Blood Maxi Kit provides silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Maxi Kit is designed for processing 3–10 ml fresh or frozen human whole blood. The QIAamp Maxi spin columns can be easily processed in a centrifuge or on vacuum manifolds (Additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with the vacuum manifolds).
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").
QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table).
No phenol–chloroform extraction is required. The DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.
The QIAamp DNA Blood Maxi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Maxi Kit processes sample sizes of 3–10 ml, with a preparation time of 55 minutes. The typical yield from 10 ml healthy whole blood is 300–600 µg, with an elution volume of 500–2000 µl.
The QIAamp DNA Blood Maxi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Maxi spin columns with 50 ml collection tubes. Sample sources include:
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