miRNA expression analysis

MicroRNA (miRNA) expression profiling simultaneously compares the expression levels of multiple or single miRNAs between two or more samples. This analysis can help scientists identify and quantify miRNA as a biomarker in diseases such as cancer. It provides valuable insight into the role of miRNA expression in normal biological states and diseases.

Benefits of nanoplate dPCR for miRNA expression analysis

  • Discrimination of single nucleotide differences in closely sequence-related miRNAs thanks to the high specificity of dPCR
  • Absolute quantification of subtle miRNA expression changes, especially in low template amounts

Analysis of miRNAs in defined cell pools and single cells using digital PCR

In this application note, read about a combination of both cellenONE and QIAcuity technology for high-throughput absolute quantification of miRNAs in well-defined cell pools and on a single-cell level. Learn how FastLane lysis buffers reduced hands-on time and the EG-based chemistry of QIAcuity allowed for miRNA analysis without major optimization. Explore the entire workflow from cell sorting with cellenONE technology to miRNA quantification with the QIAcuity to achieve sensitive, reproducible and linear quantification from cell lysates as RT and PCR input. 

Read application note​

Further resources

Martorana D et al. Absolute quantification of miRNAs with high accuracy and precision using digital PC. QIAGEN, 2023.