RNeasy Mini Kit

For purification of up to 100 µg total RNA from cells, tissues, and yeast

Fast procedure delivering high-quality total RNA in minutes
Ready-to-use RNA for high performance in any downstream application
Consistent RNA yields from small amounts of starting material
No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 μg RNA. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube Connect. For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

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製品の詳細
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関連資料

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製品名		Cat. no.	List price:
RNeasy Mini Kit (50) 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers		74104	¥36,000
RNeasy Mini Kit (250) 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers		74106	¥157,000

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RNeasy Mini Kit は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。

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RNeasy Mini操作手順|各種サンプルから得られた高品質RNA|わずか100個の細胞から得られたRNAのRT-PCR|RNeasy Mini Spin Column|

RNeasy Mini Kitを用いて精製したトータルRNAは、高品質であり、多くのダウンストリームアプリケーションに適している。プロトコールは、部分的に精製したRNA、in vitro転写物、酵素反応液から得られたRNAのクリーンアップにも含まれている。酵母サンプルに必要なlyticase、zymolase、ガラスビーズは付属されていない。サンプルから精製されるRNAの量は、サンプルソースの発達段階、種、増殖条件により異なる可能性がある。RNeasyは200塩基以上のRNAのみを精製するので、精製物には5S rRNA、tRNAやその他の低分子RNAを含まない。|RNeasy Maxi Kitで精製したトータルRNAのホルムアルデヒドアガロースゲルおよびノーザンブロット。各種サンプルから分離したトータルRNA 10 µgを各レーンにロードした。全ての組織はマウスから採取した。酵母：Saccharomyces cerevisiae、大腸菌株：HB101。³²P標識プローブは、（G）GAPDH、（E）翻訳伸長因子EF-1α、（O）外膜タンパク質Aの配列を認識した（データ提供：EはP. Philippsen, University of Basel, Switzerland、OはU. Henning, Max Planck Institute of Biology, Tübingen, Germany）。B.subtilisは調査しなかった。M：0.24～9.5kb RNAラダー。胎児、Huh7、およびHeLa細胞レーンの7.5 kbバンド（矢印）は核内RNA前駆体。|表記の数のHeLa細胞からRNeasy Mini Kitを用いて精製したトータルRNAのRT-PCR。溶出液10 µl（1/5）は、RNaseフリーDNaseで分解後、オリゴ-dTプライマーを用いて逆転写反応を行なった。このcDNAミックスの2.5 µl（1/20）を50 µlのPCR反応に使用した。GAPDHのA 452 bpフラグメントが増幅された。C-：ネガティブコントロール、C+：ポジティブコントロール、M：100 bpラダー。|RNeasy Mini Kitに添付されているRNeasy Mini Spin Column。|

パフォーマンス

Total RNA is easily purified with the RNeasy Mini Kit from up to 30 mg animal or human tissues, from 100 to 1 x 10⁷ animal or human cells, or yeast (see table; and figures "RT-PCR of RNA from as few as 100 cells" and "High-quality RNA from a variety of samples").

Total RNA yields obtained with the RNeasy Mini Kit
Source	Starting material	Average yield (µg)
Animal cells LMH HeLa COS-7 Lymphocytes (unstimulated)	1 x 10⁶1 x 10⁶1 x 10⁶1 x 10⁶	12 15 35 0.5
Mouse tissue Liver Lung Spleen	10 mg 10 mg 10 mg	40 10 35
Yeast cells S. cerevisiae	1 x 10⁷	25

原理

The RNeasy Mini Kit allows efficient purification of total RNA from small amounts of starting material. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA.

操作手順

With the RNeasy Mini Kit, total RNA is easily purified from 10 to 1 x 10⁷ animal or human cells, 0.5–30 mg animal or human tissues, and <5 x 10⁷ yeast cells. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (see figure "RNeasy Mini spin column"). RNA binds (up to 100 µg capacity), and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in 30–100 µl water (see figure "RNeasy Mini procedure"). A variety of special application protocols is also available.

アプリケーション

RNA purified with RNeasy technology has A_260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5) and is ideal for use in all applications. Downstream applications include:

Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A+ RNA selection

Feature	Specifications
Applications	Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume	30–100 µl
Format	Spin column
Main sample type	Tissue, cells
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Total RNA
Sample amount	0.5–30 mg
Technology	Silica technology
Time per run or per prep	20 minutes
Yield	Varies

パンフレット

Sort options アルファベット順
	All insights start with the sample: Your comprehensive guide for isolating top-quality RNA	詳細を表示
	(EN) - Analyzing Gene Expression and Regulation	詳細を表示
	(JA) - 遺伝子発現および発現制御の解析	詳細を表示
	RNA Functional Analysis – enhanced by LNA	詳細を表示
	Product Profile - RNeasy® Mini, Midi and Maxi Kits	詳細を表示

FAQ

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	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	表示
	How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32	表示
	Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97	表示
	How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28	表示
	Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101	表示
	How should RNeasy Kits be stored and how long are they stable? FAQ ID -103	表示
	How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113	表示
	What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139	表示
	Are RNeasy spin columns sold separately? FAQ ID -159	表示
	How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023	表示
	How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024	表示
	Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026	表示
	Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage? FAQ ID -1037	表示
	What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043	表示
	Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit? FAQ ID -1054	表示
	How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087	表示
	Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits? FAQ ID -1164	表示
	Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit? FAQ ID -1207	表示
	Do you have a protocol for purification of cytoplasmic RNA from animal cells? FAQ ID -1257	表示
	What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1616	表示
	Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619	表示
	What sample types can be used with the AllPrep DNA/RNA 96 Kit? FAQ ID -1996	表示
	Can I clean up my DNase treated RNA samples using RNeasy columns? FAQ ID -286	表示
	What is the maximum binding capacity of RNeasy spin columns? FAQ ID -290	表示
	How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit? FAQ ID -2192	表示
	Effects of low A260/A230 ratios in RNA preparations on downstream applications FAQ ID -2248	表示
	What is the key technical challenge in isolating high quality RNA from cell or tissue samples? FAQ ID -2656	表示
	What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments? FAQ ID -2657	表示
	What should I do if I suspect that my RNA preparation contains RNase contamination? FAQ ID -2661	表示
	What is the composition of Buffer RLT? FAQ ID -2793	表示
	What is the composition of Buffer RW1? FAQ ID -2796	表示
	What is the composition of Buffer RPE? FAQ ID -2797	表示
	What is the composition of Buffer RDD? FAQ ID -2800	表示
	How much RNA does a typical mammalian cell contain? FAQ ID -2946	表示
	What is the composition of PBS? FAQ ID -361	表示
	I accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do? FAQ ID -364	表示
	Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells? FAQ ID -372	表示
	Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately? FAQ ID - 3388	表示
	What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit? FAQ ID - 3519	表示
	Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues? FAQ ID -467	表示
	How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit? FAQ ID -468	表示
	What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485	表示
	Can QIAquick Kits be used to clean up RNA samples? FAQ ID -490	表示
	What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed? FAQ ID -514	表示
	What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528	表示
	What is Tissue-Tek O.C.T., and what is it used for? FAQ ID -530	表示
	Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532	表示
	Do you have a protocol for isolating RNA from yeast using RNeasy? FAQ ID -535	表示
	Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution? FAQ ID -619	表示
	How can I improve my RNA yield from liver sample when using RNeasy Kits? FAQ ID -623	表示
	How can I minimize degradation of RNA from my pancreas sample? FAQ ID -624	表示
	Do you have a kit for RNA isolation from any kind of sample type? FAQ ID -627	表示
	What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample? FAQ ID -631	表示
	What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728	表示
	I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20	表示
	I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745	表示
	Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA? FAQ ID -785	表示
	Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit? FAQ ID -797	表示
	Do I need to use RNase inhibitors with the RNeasy Kits? FAQ ID -813	表示
	Why is it not recommended to stabilize cells with RNAprotect Tissue Reagent? FAQ ID -941	表示
	What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942	表示
	Do you have a protocol for the isolation of RNA from leukocytes in milk? FAQ ID -952	表示
	How do I clean up RNA preparations containing miRNA? FAQ ID -3002	表示
	Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit? FAQ ID - 3623	表示

キットハンドブック

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	(JA) - RNeasy Miniプロトコールとトラブルシューティング RNeasy Mini Kit －動物細胞、動物組織、バクテリア、酵母からのトータルRNA精製およびRNAクリーンアップ用 RNeasy Protect Mini Kit －採取した動物組織からのRNAの迅速な安定化と続くトータルRNA精製 RNeasy Plant Mini Kit －植物および糸状菌類からのトータルRNA精製用	詳細を表示
	RNeasy Mini Handbook - (EN) RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi	詳細を表示

Supplementary Protocols

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	(JA) - マイクロダイセクション用特別プロトコール - RNA分離マイクロダイセクションにより採取した組織サンプルからRNeasy Mini Kit を用いたRNA 分離	詳細を表示
	Acetone precipitation of protein from Buffer RLT or Buffer RLT Plus lysates - (EN)	詳細を表示
	Purification of cytoplasmic RNA from animal cells using the RNeasy® Mini Kit - (EN)	詳細を表示
	Purification of total RNA from bacteria using the RNeasy® Mini Kit - (EN) Up to 1 x 10⁹ bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.	詳細を表示
	Isolation of Peripheral Blood Mononuclear Cells (PBMC) and Purification of Total RNA from PBMC Using the RNeasy® Micro or Mini Kit - (EN)	詳細を表示

User-Developed Protocols

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	Isolation of RNA from leukocytes in milk using QIAGEN® RNeasy® Kits - (EN) One milliliter of milk contains approximately 50,000-200,000 leukocytes (with an average of approximately 100,000 leukocytes). In a BVDV-infected animal up to 1-30% of the leukocytes may be infected with the virus. Each infected leukocyte typically contains 10-10,000 BVDV entities.	詳細を表示

Application Notes

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	Sequential automation of RNA and DNA preps on the same QIAcube instrument	詳細を表示
	(EN) - Purification of total RNA from peripheral blood mononuclear cells	詳細を表示

クイックスタートプロトコール

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	RNeasy Mini Kit, Part 1 (EN)	詳細を表示
	RNeasy Mini Kit, Part 2 (EN)	詳細を表示

MSDS

Sort options アルファベット順
	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	表示

サイエンティフィック・ポスター

Sort options アルファベット順
	Explore the RNA Universe! Poster for download	詳細を表示

安全データシート

Sort options アルファベット順
	MSDS RNeasy Mini Kit (50)
	MSDS RNeasy Mini Kit (250)
	CofA

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RNeasy Mini操作手順

High-quality RNA from a variety of samples.

各種サンプルから得られた高品質RNA

RNeasy Maxi Kitで精製したトータルRNAのホルムアルデヒドアガロースゲルおよびノーザンブロット。各種サンプルから分離したトータルRNA 10 µgを各レーンにロードした。全ての組織はマウスから採取した。酵母：Saccharomyces cerevisiae、大腸菌株：HB101。³²P標識プローブは、（G）GAPDH、（E）翻訳伸長因子EF-1α、（O）外膜タンパク質Aの配列を認識した（データ提供：EはP. Philippsen, University of Basel, Switzerland、OはU. Henning, Max Planck Institute of Biology, Tübingen, Germany）。B.subtilisは調査しなかった。M：0.24～9.5kb RNAラダー。胎児、Huh7、およびHeLa細胞レーンの7.5 kbバンド（矢印）は核内RNA前駆体。

わずか100個の細胞から得られたRNAのRT-PCR

表記の数のHeLa細胞からRNeasy Mini Kitを用いて精製したトータルRNAのRT-PCR。溶出液10 µl（1/5）は、RNaseフリーDNaseで分解後、オリゴ-dTプライマーを用いて逆転写反応を行なった。このcDNAミックスの2.5 µl（1/20）を50 µlのPCR反応に使用した。GAPDHのA 452 bpフラグメントが増幅された。C-：ネガティブコントロール、C+：ポジティブコントロール、M：100 bpラダー。

RNeasy Mini Spin Column

RNeasy Mini Kitに添付されているRNeasy Mini Spin Column。