QIAquick Gel Extraction Kit

ゲル抽出あるいは酵素反応液からの最高10 µg までのDNA（70 bp～10 kb）クリーンアップ用

即使用可能なDNAの回収率が最高95％
迅速で簡便な操作
簡単な3ステップで最大10 kbまでのDNAをクリーンアップ
ゲル解析をより簡便化するGel Loading Dye付き

QIAquick Gel Extraction Kitは、スピンカラム、バッファー、コレクションチューブにより構成され、シリカメンブレンによりDNAフラグメントを400 mgまでのゲル切片あるいは酵素反応液から精製します。簡便で迅速な結合·洗浄·溶出ステップにより、70 bp～10 kbのDNAを30～50 µlの溶出液を用いて精製できます。pH指示薬入りバッファーにより、DNAがスピンカラムに結合する最適なpHを簡単にチェックできます。本キットはQIAcubeで完全自動化が可能です。

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Product Details
Specifications
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Product	Cat. no.	List price:
QIAquick Gel Extraction Kit (50) For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28704	115,00 €	Add to cart
QIAquick Gel Extraction Kit (250) For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28706	527,00 €	Add to cart
QIAquick Gel Extraction Kit (1000) For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)	28706X4	1.674,00 €	Add to cart
QIAquick PCR & Gel Cleanup Kit (100) For purification PCR fragments and gel extractions of 100 reactions (at least 80–100 gel reactions or 100 PCRs)	28506	208,00 €	Add to cart
QIAquick Spin Columns (100) For DNA fragment purification from PCR, gel fragments and enzymatic reactions	28115	113,00 €	Add to cart

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The QIAquick Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
|DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A] 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B] 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit is shown.
|

Performance

QIAquick Gel Extraction Kitは、ヌクレオチド、酵素、塩類、アガロース、臭化エチジウムなどの夾雑物をサンプルから除去し、DNAを最大80％まで確実に回収します（図"ゲルからの高回収率"）。マイクロ遠心機または吸引マニホールドを使用すれば、1～24サンプルから70 bp～10 kbのDNAが精製されます。精製したDNAは、シークエンシングなどで使用することができます（図"ゲル抽出後の信頼性あるシークエンシング"）。70 bp以下または10 kb以上のDNAフラグメントの精製には、QIAEX II Gel Extraction Systemをご利用ください。

Principle

QIAquick Kitでは、高塩濃度バッファーによりDNAを結合し、低塩濃度バッファーあるいは水によりDNAを溶出するシリカゲルメンブレンを利用しています。この精製法でDNAサンプルからプライマー、ヌクレオチド、酵素、ミネラルオイル、塩、アガロース、臭化エチジウム、およびその他の夾雑物が除去できます（図 “ゲルからの高回収率”）。シリカメンブレンテクノロジーにより樹脂漏れ、および懸濁液関連の問題や不便さは解消されます。それぞれの用途に応じて至適化された結合バッファーにより、種々の大きさのDNAフラグメントを選択的に吸着します。

Gel Loading Dye

GelPilot Loading Dye は3 種類のマーカー色素（xylene cyanol、bromophenol blueおよびorange G）が入っており、短いDNAフラグメントのゲルからの流出を回避でき、アガロースゲルの泳動時間の至適化が簡単に行なえます（図 "GelPilot Loading Dye"）。

Procedure

QIAquickシステムでは結合－洗浄－溶出という簡単な操作だけでDNAのクリーンアップが可能です（フローチャート"QIAquickおよびMinEluteの操作手順"参照）。ゲル切片をpH指示薬を含んだバッファーに溶解させると、DNA結合の至適pHを容易に確認できます。 DNAを含むバッファーをQIAquick Spin Column にアプライします（図 "pH 指示薬"）。DNAは添付のバッファー中の高塩濃度の条件下でシリカゲルメンブレンに吸着します。不純物は洗い流され、純粋なDNAを添付の低塩濃度溶出バッファー、あるいは水で溶出します。得られたDNAは、幅広いアプリケーションに即使用可能です。

操作法

QIAquick Spin Column は2つの簡便な操作法オプションのためにデザインされました。スピンカラムは簡便な卓上型マイクロ遠心機あるいはQIAvac 24 Plus のようなルアーコネクター付き吸引装置で使用できます。QIAquick Gel Extraction Kitは他のQIAGENスピンカラムを利用したキット同様にQIAcubeでの完全自動化が可能で、標準化された再現性の高い結果が得られます（図 "Spin Column操作オプションA、 B、 C、D、およびE"）。

Applications

QIAquickシステムで精製したDNAフラグメントは、シークエンシング、ライゲーション、トランスフォーメーション、制限酵素解析、標識反応、マイクロインジェクション、PCR、in vitro転写反応などのアプリケーションに即使用可能です。

Feature	Specifications
Binding capacity	10 µg
Elution volume	30–50 µl
Format	Tube
Fragment size	70 bp – 10 kb
Processing	Manual
Recovery: oligonucleotides dsDNA	Recovery: dsDNA fragments
Removal <10mers 17–40mers dye terminator proteins	Removal <10mers
Sample type: applications	DNA: PCR reactions
Technology	Silica technology

FAQs

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	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	View
	How can I extract DNA from a polyacrylamide (PAGE) gel? FAQ ID -120	View
	Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel? FAQ ID -133	View
	What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick? FAQ ID -148	View
	How can I improve recoveries when using the QIAquick Kits? FAQ ID -180	View
	What is the composition of Buffer EB? FAQ ID -199	View
	Why does my DNA sample float out of the slot when loading it onto an agarose gel? FAQ ID -205	View
	Can I buy QIAquick and MinElute columns separately? FAQ ID -2460	View
	How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305	View
	Are QIAprep and QIAquick Spin columns interchangeable? FAQ ID -311	View
	Can I store agarose gel slices containing DNA for gel extraction at a later point? FAQ ID -313	View
	Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits? FAQ ID -519	View
	Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable? FAQ ID -577	View
	Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing? FAQ ID -637	View
	I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost? FAQ ID -756	View
	Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns? FAQ ID -759	View
	Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable? FAQ ID -786	View
	Do you have protocols for multiple extractions of DNA fragments from agarose gels? FAQ ID -944	View

Scientific Posters

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	6 secrets to optimize your gel extraction results Poster	Show details

Quick-Start Protocols

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	QIAquick Gel Extraction Kit and QIAquick PCR & Gel Cleanup Kit - (EN)	Show details

Kit Handbooks

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	QIAquick Spin Handbook - (EN)	Show details

User-Developed Protocols

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	Extraction of DNA fragments from polyacrylamide gels using the QIAquick® Gel Extraction Kit - (EN)	Show details

Safety Data Sheets

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	View

Safety Data Sheets

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	MSDS QIAquick Gel Extraction Kit (50)
	MSDS QIAquick Gel Extraction Kit (250)
	MSDS QIAquick Gel Extraction Kit (1000)
	MSDS QIAquick PCR & Gel Cleanup Kit (100)
	MSDS QIAquick Spin Columns (100)
	CofA

Images

QIAquick 96 on QIAvac 96/BioRobot systems

QIAquick and MinElute procedure.

The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.

High recoveries from gels.

DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A] 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B] 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.

GelPilot Loading Dye.

GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

pH Indicator Dye.

pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.

Spin column handling options — D.

QIAvac 24 plus.

Spin column handling options — E.

QIAcube.

Spin column handling options — C.

Manifold with luer connectors.

Spin column handling options — B.

QIAvac 24.

Spin column handling options — A.

Microcentrifuge.

Reliable sequencing after gel extraction.

Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit is shown.