MinElute Reaction Cleanup Kit
For cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions
- Very small elution volumes
- Fast procedure and easy handling
- High, reproducible recoveries
- Gel loading dye for convenient sample analysis
The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
en-CA
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Product
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Cat. no.
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List price:
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MinElute Reaction Cleanup Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2 :ml)
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28204
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MinElute Reaction Cleanup Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
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28206
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The MinElute Reaction Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
MinElute操作手順|GelPilot Loading Dye|MinEluteメンブレン構造|pH 指示薬|Spin Column操作オプション - D|Spin Column操作オプション - E|Spin Column操作オプション - C|Spin Column操作オプション - B|Spin Column操作オプション - A|
この結合―洗浄―溶出の簡単な操作によって、簡便性が確実に高まる。 |GelPilot Loading Dye に入っている3種類のマーカー色素によりDNA電気泳動の至適化が容易。
|ユニークなメンブレン構造を持つMinElute Spin Column断面図(utility model pending)。|溶解および結合バッファー中のpH指示薬により、DNA吸着の至適pH(pH <7.5)かどうかを簡単に確認できる。アガロースゲル電気泳動バッファーを繰り返し使用したり、間違って調製した場合に、結合溶液のpHは至適pHより高くなる。このような場合は、3M 酢酸ナトリウム、pH 5.0 を10 µl 添加してpHを簡単に調整することが可能。|QIAvac 24 Plus|QIAcube |ルアーコネクター付きマニホールド |QIAvac 24 |マイクロ遠心機 |
パフォーマンス
The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The kit provides spin columns for cleanup of enzymatic reactions. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick System.)
| DNA Polymerase I |
109 |
| Klenow fragment |
62 |
| Calf intestinal alkaline phosphatase |
69 |
| T4 DNA ligase |
55 |
| T4 Polynucleotide kinase |
35 |
| Terminal transferase |
32 |
| DNase I |
31 |
| Restriction enzymes |
Varies |
原理
MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.
Gel loading dye
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").
操作手順
The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Handling
MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters. The MinElute Reaction Cleanup Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").
アプリケーション
DNA fragments purified with the MinElute System are ready for direct use in all applications, including:
- Sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labeling
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Feature
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Specifications
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Binding capacity
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5 µg
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Elution volume
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10 µl
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Format
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Tube
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Fragment size
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70 bp – 4 kb
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Processing
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Manual
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Recovery: oligonucleotides dsDNA
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Recovery: oligonucleotides, dsDNA
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Removal <10mers 17–40mers dye terminator proteins
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Removal <40mers
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Sample type: applications
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DNA, oligonucleotides: Enzymatic reactions
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Technology
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Silica technology
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Download Safety Data Sheets for QIAGEN product components.
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View
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Images
MinElute procedure.
This simple bind–wash–elute procedure ensures greater convenience.
GelPilot Loading Dye.
GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
MinElute membrane assembly.
MinElute spin column in cross section, showing the unique membrane assembly (utility model pending).
pH Indicator Dye.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
Spin column handling options — D.
QIAvac 24 plus.
Spin column handling options — E.
QIAcube.
Spin column handling options — C.
Manifold with luer connectors.
Spin column handling options — B.
QIAvac 24.
Spin column handling options — A.
Microcentrifuge.
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