For fractionation of proteins according to cellular location
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Qproteome Cell Compartment Kit
For up to 10 subcellular fractionations*: Extraction buffers, Protease Inhibitor Solution, Benzonase®.

Qproteome Cell Compartment Kit  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。

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Localization of proteins in cells under different growth conditions.|Flowchart.|Specific separation of marker proteins.|Localization of proteins in tissue samples.|
Micrographs (upper panels) of HeLa cells [A] under normal growth conditions and [B] after 4 hour incubation with staurosporine, an inducer of apoptosis. Lower panels show western blots prepared after cell lysates were processed using the Qproteome Cell Compartment Kit. The blots were probed with anti-cytochrome c antibody. It is known that during apoptosis cytochrome c is translocated from the intermembrane space of mitochondria to the cytosol, a process that is reflected in the detection of cytochrome c in the cytosolic fraction of apoptotic cells.||Western blots of fractionated NIH 3T3 cells. Protein (20 µg) from the cytosolic (C), membrane (M), and nuclear (N) fractions was separated by SDS-PAGE. After Western blotting, proteins specific to each fraction were detected using  [A] annexin,  [B] TIM23, and  [C] lamin antibodies, and an HRP-conjugated secondary antibody with chemiluminescent detection.|The indicated rat tissue was processed using the Qproteome Cell Compartment Kit. The respective fractions (C: cytosol, M: membrane, N: nucleus, and S: cytoskeleton) were separated by SDS-PAGE and transferred by western blotting to nitrocellulose membrane. The blot was probed with antibodies against the indicated cell-compartment-specific marker proteins.|
Use of the Qproteome Cell Compartment Kit allows simple and effective cell compartment-specific localization of marker proteins from isolated cells (see figure "Specific Separation of Marker Proteins" and to  "Localization of Proteins in Cells Under Different Growth Conditions") or from tissue samples (see figure "Localization of Proteins in Tissue Samples").

The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues.


The 4 Extraction Buffers are added sequentially to a cell pellet and the respective fractions are isolated by centrifugation (see flowchart).

In an alternative protocol for tissues, samples are simultaneously homogenized and disrupted using the TissueRuptor before being processed as for the cell pellet.


Subcellular fractionation of proteins can be used for the enrichment of low-abundance species; to define the subcellular localization of enzymes, regulatory, and structural proteins; and for monitoring of compartmental redistribution of biomolecules under basal and stimulated conditions.

Applications SDS-PAGE, mass spectrometry
Binding capacity/yield Varies
Fractions isolated Four fractions
Sample size ~5 x 10e6 cells
Species Eukaryotes
Start material Cell lysate

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