For highly sensitive and specific detection of GST-tagged proteins
  • Mouse monoclonal antibodies
  • Highly sensitive and specific detection
  • Sensitivity in western blots of up to 0.5 ng
  • No cross-reactivity to proteins from various other species
GST-tag Antibody allows immunologic applications such as western blot, enzyme-linked immunosorbent assay (ELISA), and immunoprecipitation. The antibody is highly sensitive and allows detection of amounts of GST protein as low as 0.5 ng.
製品名 Product no. Cat. no. List price:
 
 
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
製品名 Cat. no. List price:
GST-tag Antibody (100 µg)
100 μg anti-GST-tag antibody (lyophilized, BSA-free)
34860
¥72,500

GST-tag Antibody  は分子生物学実験用です。疾病の診断、治療または予防の目的には使用することはできません。


  • Main Image Navi
Sensitivity and specificity of GST-tag Antibody.|
[A] Slot blot. The indicated amounts of purified GST protein in denaturing buffer were slot blotted onto nitrocellulose membrane, probed with GST-tag Antibody at 1:1000 dilution, and detected using chemiluminescence (1 minute exposure). As little as 0.5 ng could be detected under these conditions. [B] Western blot. 100 ng of GST was mixed with 50 μg of the lysate derived from the indicated cells, separated by SDS-PAGE, and blotted onto nitrocellulose membrane. The membrane was probed with GST-tag Antibody at 1:1000 dilution and detected using chemiluminescence (1 minute exposure).|
パフォーマンス
The highly specific and sensitive GST-tag Antibody enables detection of even low amounts of GST-tagged proteins, up to 0.5 ng in western blots (see figure "Sensitivity and specificity of GST-tag Antibody").
原理
The GST-tag is a versatile tool for purification and detection of proteins. The monoclonal antobodies have no cross-reactivity with proteins from various other species, giving highly sensitive and specific detection (see figure "Sensitivity and specificity of GST-tag Antibody").
操作手順
Proteins expressed with a GST-tag are readily isolated from a lysate using Glutathione HiCap Matrix. The purity of the GST-tagged protein can be confirmed by western blotting. After transfer of the isolated protein to the membrane, this is incubated with a blocking solution to minimize non-specific background staining. The membrane is subsequently incubated with GST-tag Antibody diluted appropriately, followed by secondary antibody specific for the GST-tag Antibody. The secondary antibody is conjugated to allow chromogenic or chemiluminescent detection (see figure "Sensitivity and specificity of GST-tag Antibody").
アプリケーション

GST-tag Antibody allows immunologic applications, such as:

  • Western blot
  • Slot blot
  • Enzyme-linked immunosorbent assay (ELISA)
  • Immunoprecipitation
Feature
Specifications
Applications Western blot, dot/slot blot, ELISA, immunoprecipitation
Detection secondary antibody required
Epitope detected Glutathione S-transferase from Schistosoma japonicum
Sensitivity in Western blots (chemiluminescent detection) 0,5 ng
Substrate for blot detection dependant on secondary antibody
Substrates for assay procedure dependant on secondary antibody
Tag GST-tag

You are not authorized to download the resource

安全データシート
1