RNeasy Plus Universal Kits

For purification of total RNA from all types of tissue using gDNA Eliminator Solution
  • One-for-all tissue solution
  • Time-saving integration of RNeasy and QIAzol technologies
  • Fast non-enzymatic removal of genomic DNA
  • Pure RNA for use in downstream applications

RNeasy Plus Universal Kits integrate fast, convenient RNA purification with effective elimination of genomic DNA. Optimized protocols enable purification of high-quality RNA from any type of tissue, even difficult-to-lyse tissues. The kits include QIAzol Lysis Reagent for lysing fatty tissue and other types of tissue and RNeasy spin columns for purifying high-quality RNA. Kits are available in two formats: the RNeasy Plus Universal Mini Kit enables purification of RNA from up to 50 mg tissue and the RNeasy Plus Universal Midi Kit enables purification of RNA from up to 250 mg tissue. The RNeasy Plus Universal Mini Kit can be automated on the QIAcube Connect.

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产品 货号 目录价:
RNeasy Plus Universal Mini Kit (50)
For 50 RNA minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
73404
询价
RNeasy Plus Universal Midi Kit (10)
For 10 RNA midipreps: RNeasy Midi Spin Columns, gDNA Eliminator Solution, Collection Tubes, RNase-Free Water and Buffers
73442
询价

RNeasy Plus Universal Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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高效去除基因组DNA。|高品质RNA。|RNeasy Plus Universal Mini和Midi实验流程。|
使用RNeasy Plus Universal Mini Kit从图示量的RNAlater稳定的大鼠肾脏中分离RNA,或者使用Supplier AII的沉淀试剂进行沉淀。使用QuantiFast Probe RT-PCR Kit和Rotor-Gene Q实时荧光定量PCR分析仪对RNA洗脱液进行real-time RT-PCR分析,包括或不包括逆转录步骤。|使用RNeasy Plus Universal Mini Kit,从加入或者不加入gDNA Eliminator Solution的大鼠组织中纯化RNA。[A] 使用从25 mg冷冻的肝脏组织中分离的RNA进行real-time RT-PCR分析。所有样本的CT值相当。如果未使用gDNA Eliminator Solution,则在–RT对照中可检测到DNA污染,而使用gDNA Eliminator Solution分离的样本中则几乎无DNA污染。[B] 从25 mg稳定的心脏组织中纯化RNA。使用QIAxcel系统获取的数据证明,无论是否使用gDNA Eliminator Solution分离RNA,RNA均具有极佳的完整性。||
性能

The RNeasy Plus Universal procedure allows high, reproducible RNA yields from any type of tissue, including fatty tissues and difficult-to-lyse tissue (see table). Yields can vary due to factors such as species and developmental stage, especially with adipose tissues.

Typical yields of total RNA with RNeasy Plus Universal Kits
Mouse/rat tissue (10 mg)Yield of total RNA ((µg)
Adipose tissue 0.5–2.5
Brain 5–20
Heart 5–25
Intestine 10–60
Kidney 5–40
Liver 15–80
Lung 5–15
Muscle 5–35
Skin 2–5
Spleen 15–100
Analysis of purified RNA by real-time RT-PCR, and on the QIAxcel system, demonstrates high-quality RNA with good  integrity (see figure "High-quality RNA"). gDNA Eliminator Solution and RNeasy technologies efficiently remove most of the genomic DNA contamination without DNase treatment (see figure "Effective removal of genomic DNA").

原理

The RNeasy Plus Universal procedure enables purification of RNA from any type of tissue, including fatty or difficult-to-lyse tissues. RNeasy Plus Universal Kits integrate phenol/guanidine-based sample lysis and silica-membrane purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of all kinds of tissues and to inhibit RNases. The highly efficient lysis and the subsequent removal of contaminants by organic phase extraction enable the use of larger amounts of tissue with RNeasy spin columns. gDNA Eliminator solution effectively removes genomic DNA contamination and significantly improves separation of DNA into the interphase.

操作流程

The RNeasy Plus Universal procedure is easy to follow and, compared to phenol/chloroform treatment with a subsequent cleanup procedure, much faster and more efficient with better RNA purity and yield (see flowchart "RNeasy Plus Universal Mini and Midi procedures"). Total RNA is purified from up to 50 mg tissue using the RNeasy Plus Universal Mini Kit or up to 250 mg tissue using the RNeasy Plus Midi Kit.

Tissue samples are homogenized in QIAzol Lysis Reagent using the TissueRuptor, TissueLyser LT, or TissueLyser II. After addition of gDNA Eliminator Solution and chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase with RNA is collected, mixed with ethanol, and RNA is purified using RNeasy spin columns. Total RNA binds to the spin column membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is eluted in RNase-free water.

The procedure provides an enrichment for mRNA since most RNAs <200 nucleotides (such as rRNAs and tRNAs) are excluded. The RNeasy Plus Universal procedure can be modified to allow the purification of total RNA containing small RNAs, such as miRNA, from cultured cells. 

应用

RNA purified using the RNeasy Plus Universal Kits is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as quantitative real-time RT-PCR. The purified RNA can also be used in other applications.

特点
参数
应用 PCR, real-time PCR, microarray
洗脱体积 Mini: 30µl; Midi:150µl
自动化处理 Mini format can be automated on the QIAcube
规格 Mini and Midi Format
整合基因组DNA去除步骤 Yes
主要样本类型 Tissue samples including difficult-to-lyse and demanding tissues
处理 Manual (centrifugation), automated (QIAcube)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 RNA
样本量 Mini: 5-50 mg; Midi: 50-250 mg
技术 QIAzol/Spin column technology

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キットハンドブック
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For purification of total RNA from all types of tissue
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RNeasy Plus Universal Mini Kit
RNeasy Plus Universal Midi Kit すべてのタイプの組織からのトータルRNA 精製
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图片
Effective removal of genomic DNA.
高效去除基因组DNA。
使用RNeasy Plus Universal Mini Kit从图示量的RNAlater稳定的大鼠肾脏中分离RNA,或者使用Supplier AII的沉淀试剂进行沉淀。使用QuantiFast Probe RT-PCR Kit和Rotor-Gene Q实时荧光定量PCR分析仪对RNA洗脱液进行real-time RT-PCR分析,包括或不包括逆转录步骤。
High-quality RNA
高品质RNA。
使用RNeasy Plus Universal Mini Kit,从加入或者不加入gDNA Eliminator Solution的大鼠组织中纯化RNA。[A] 使用从25 mg冷冻的肝脏组织中分离的RNA进行real-time RT-PCR分析。所有样本的CT值相当。如果未使用gDNA Eliminator Solution,则在–RT对照中可检测到DNA污染,而使用gDNA Eliminator Solution分离的样本中则几乎无DNA污染。[B] 从25 mg稳定的心脏组织中纯化RNA。使用QIAxcel系统获取的数据证明,无论是否使用gDNA Eliminator Solution分离RNA,RNA均具有极佳的完整性。
RNeasy Plus Universal Mini and Midi Procedures.
RNeasy Plus Universal Mini和Midi实验流程。