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Product
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Cat. no.
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List price:
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QIAgility System HEPA/UV (incl. PC)
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts.
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9001532
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QIAgility, HEPA/UV, PC, Priority
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.
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9001903
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QIAgility, HEPA/UV, PC, PrioPLUS
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training, Full Agreement for three years with a two business day response time and three preventive maintenance visits.
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9001904
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QIAgility HEPA/UV (w/o PC) System
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.
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9001908
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QIAgility HEPA/UV (w/o PC), PrioPLUS
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training, Full Agreement for three years with a two business day response time and three preventive maintenance visits. Does not include a laptop.
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9001910
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QIAgility HEPA/UV (w/o PC), Priority
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits. Does not include a laptop.
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9001909
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QIAgility HEPA/UV Platform System PRV-1
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer and QIAgility software; includes installation, application training and one preventive maintenance visit. One-year warranty on labor, travel and parts are also included.
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9002887
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QIAgility HEPA/UV System PRV-1
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one preventive maintenance visit. One-year warranty on labor, travel and parts also included. Does not include a laptop.
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9002888
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QIAgility HEPA/UV (w/o PC) System
Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training and one-year warranty on labor, travel and parts. Does not include a laptop.
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9001908
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QIAgility, Premium Agreement
On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of next business day. Includes one Preventive Maintenance or Inspection Service during the Premium Agreement period.
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9241764
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QIAgility, Full Agreement
On-site QIAgility instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.
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9241763
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QIAgility, Preventive Subscription
One on-site Preventive Maintenance or Inspection Service visit for the QIAgility, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.
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9241776A
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QIAgility, Core Agreement
For the QIAgility, one on-site repair service and one on-site Preventive Maintenance or Inspection Service, including travel, labor and spare parts for a period of one year. Response time of five business days. Includes a 10% discount on additional repair service during the Core Agreement period.
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9245243
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The is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Feature
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Specifications
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Accessories (supplied)
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2 x Adapter, 96 x 0.2 ml PCR Tubes
1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base
1 x Adapter, 72 x 0.1 ml Strip Tubes
1 x Master Mix Block, 5 x 5 ml
1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml
1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml
1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes
4 x Tip Rack Holder for 96-Tip Rack
The adapters are intended to hold tubes or plates.
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Communications
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USB and RS232
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Conductive Filtered Tips
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50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
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Consumables (supplied)
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10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes
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Dimensions
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Height: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables
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Electrical
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100–240 V AC, 250 VA, 50–60 Hz
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HEPA filter
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Preinstalled dual-cartridge HEPA filter system provides positive pressure clean air within the closed hood.
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Humidity
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Operating humidity: 40–70%
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Liquid-level sensing
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Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips.
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Notebook
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QIAgility comes with a notebook computer for added flexibility.
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Pipetting head
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Single channel
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Place of operation
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For indoor use only
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Pollution level
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2
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Precision
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CV <1%, 5–200 µl (dry-well transfer)
CV <5%, 2–5 µl (wet-well transfer)
CV <10%, 1 µl
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Software
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Supplied with unlimited user license. Free upgrades by Web download.
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Table capacity
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6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders
1 x Master Mix Block
1 x Reagent Block
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Temperature
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Operating temperature, 15–30 °C (59–86°F)
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Tip disposal
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All used tips ejected externally — no waste accumulation on the worktable
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Tip volume
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1–200 µl
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UV lamp
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Preinstalled UV lamp for worktable decontamination
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Warranty
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1-3 years warranty, depending on service package
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Weight
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41 kg (90.2 lb)
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Important note regarding new QIAgility declaration of conformity and instrument symbols
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Show details
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Show details
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For use with QIAgility and QIAgility Setup Manager Software
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Show details
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Alternative options for the CD drive on the QIAgility Notebook, Part Number 9026761
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Show details
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For use with QIAgility Operating Software version 4.17.1
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Show details
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For use with QIAgility Operating Software version 4.18
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Show details
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Instrument operating software including the QIAgility Setup Manager Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-in.
The software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiTect, QuantiFast, QuantiNova and Rotor-Gene kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.
For software installation instructions, please refer to the QIAgility Unpacking and Installation Instructions or to the QIAgility User Manual, chapter 4.4.
Important: It is not possible to install both QIAgility Setup Manager Software and QIAgility Operating Software 4.17.1 on the same computer. Please uninstall QIAgility Operating Software 4.17.1 before installing QIAgility Setup Manager Software. For more information, contact QIAGEN Technical Services.
Note: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Show details
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Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection.
For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.
Important: It is not possible to install both QIAgility Operating Software 4.17.1 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager Software before installing QIAgility Operating Software 4.17.1. For more information, contact QIAGEN Technical Services.
Note: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Show details
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Instrument operating software designed for flexible use in various applications. Use this software for all applications other than gene expression and pathogen detection. For gene expression and pathogen detection applications, we recommend using the QIAgility Setup Manager Software.
Important: It is not possible to install both QIAgility Operating Software 4.18 and QIAgility Setup Manager Software on the same computer. Please uninstall QIAgility Setup Manager before installing QIAgility Operating Software 4.18. For more information, contact QIAGEN Technical Services.
Note: QIAgility Operating Software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Show details
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These protocol files can only be used with QIAgility Operating Software version 4.17 and are not compatible with the new QIAgility Setup Manager Software.
QIAgility Setup Manager Software offers a complete solution for qPCR setup optimized for use with QIAGEN kits. If you need a list of kits currently supported by the QIAgility Setup Manager Software, QIAGEN Technical Services will be happy to help you. Visit support.qiagen.com for country-specific contact information.
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Show details
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Download Safety Data Sheets for QIAGEN product components.
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View
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Images
Exceptional pipetting precision and accuracy spanning over a broad volume range
The Artel MVS system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager Software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.
The indicated CV values (%) demonstrate that the QIAgility instrument performs within its claimed specifications and even better.
Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions
A reaction mix was prepared manually using 5 parts of 2x Rotor-Gene SYBR Green PCR Master Mix and 3 parts of RNase free water (ready-made reaction mix). An experiment was then defined in the QIAgility Setup Manager for the Rotor-Gene SYBR Green PCR Kit with a total reaction volume of 25 µl and a sample input volume of 5 µl, resulting in the transfer of 20 µl of the ready-made reaction mix. The transfers were made into pre-weighed 200 µl single tubes (n=36 per instrument). The tubes were weighed again after the transfer and the weight difference was considered to calculate the transferred volume. For each instrument, the pipetting precision was determined as CV (%). The data shows the mean of the CV values (bars; in %) ± S.D. (error bars) of each individual instrument. The pipetting precision or CV, respectively, was nearly 3 % for all instruments tested.
The indicated CV values (%) demonstrate that the QIAgility instrument has high performance rate in terms of precision and repeatability when pipetting ready-made reaction mix as shown on all 4 instruments.
Comparison of manual and automated assay setup using series dilutions
A QuantiNova SYBR Green assay setup was performed using the QIAgility operated with its Setup Manager Software and this was compared to a manual setup done in parallel. A manually prepared 1:10 dilution series from a stock of human gDNA with a concentration of 60 ng/µl was used in a ß-actin assay. Each dilution was tested in quadruplicates (n=4). The manual and QIAgility-prepared assay points were amplified in the same Rotor-Gene Q run. The data is represented as a box plot of the CT value calculated using the Q-Rex Software for each template concentration and setup employed.
A highly similar PCR performance is observed between manual and automated assay setup over a series of sample concentrations.
High system linearity of QIAgility
Human gDNA with a stock concentration of 1.5 µg/µl was diluted with the QIAgility six times in the ratio of 1:2 per dilution step using RNase free water as diluent. The dilution series was prepared in replicates of 3. The nucleic acid concentration of the dilutions was determined using the QIAxpert UV/VIS spectrophotometer (DNA QIAamp Application). The mean measured quantifications were plotted against the mean theoretical nucleic acid concentrations assuming a perfect 1:2 dilution. The resulting plot demonstrates the exceptional linearity of QIAgility dilution with a high regression coefficient of >0.999.
Quantifications of serial dilutions of gDNA prepared using the QIAgility are in high concordance with actual concentrations.
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